Collaborative Research: MRI Consortium: Development of Fiber-Coupled Stimulated Emission Depletion Microscopy (STED)

合作研究：MRI 联盟：光纤耦合受激发射损耗显微镜 (STED) 的开发

• 批准号: 1919361
• 负责人: Emily Gibson
• 金额: $ 40.12万
• 依托单位: University of Colorado at Denver-Downtown Campus
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-10-01 至 2024-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1919361&HistoricalAwards=false
• 关键词: Collaborative; Research; MRI; Consortium; Development

An award is made to the University of Colorado Boulder, University of Colorado Anschutz Medical Campus and the University of Denver to develop a unique miniature optical fiber-coupled microscope that will enable flexible imaging at unprecedented spatial resolution. Developed with a diverse team that includes engineers, physicists, and neuroscientists, this cutting-edge microscope will not only provide new fundamental and practical insights across research areas, but will also yield far-reaching impacts through intellectual property development and future commercialization and deployment in industry. The research team will use the new instrument to expand ongoing efforts to mentor and inspire young students from groups underrepresented in Science Technology Engineering and Mathematics (STEM) fields.Real-time in-brain imaging has made tremendous advances in the past few years, allowing unprecedented inquiry into how dynamic structures such as dendritic spines enable brain functions such as learning, memory, cognition, and behavior. However, future advances in observation-based understanding of the function and response of these cellular structures are limited by the blur inherent to light's fundamental properties that obscures these features. Based on the established and Nobel Prize-winning principle of Stimulated Emission Depletion (STED) microscopy, the instrument developed in this collaborative project will be capable of a 5X increase in spatial resolution. This miniature microscope will enable, for the first time, imaging of small structures such as synaptic spine dynamics and changes in myelination underlying behaviors and social interactions in behaving animals. It will also enable lithography in 3D materials at unprecedented spatial scale aimed towards the next generation of electronic devices.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

授予科罗拉多大学博尔德分校、科罗拉多大学安舒茨医学院和丹佛大学开发一种独特的微型光纤耦合显微镜，使灵活的成像具有前所未有的空间分辨率。由包括工程师、物理学家和神经科学家在内的多元化团队开发的这款尖端显微镜不仅将为各个研究领域提供新的基础和实用见解，还将通过知识产权开发和未来的商业化以及在工业中的部署产生深远的影响。研究团队将使用新仪器来扩大正在进行的努力，以指导和激励来自科学技术工程和数学（STEM）领域代表性不足的群体的年轻学生。实时脑内成像在过去几年中取得了巨大的进步，允许前所未有的调查动态结构，如树突棘如何使大脑功能，如学习，记忆，认知和行为。然而，未来在基于观察的理解这些细胞结构的功能和响应方面的进展受到光的基本特性所固有的模糊的限制，这种模糊了这些特征。基于受激发射耗尽（STED）显微镜的既定和诺贝尔奖获奖原理，该合作项目中开发的仪器将能够将空间分辨率提高5倍。这种微型显微镜将首次实现对小结构的成像，如突触脊柱动力学和行为动物中髓鞘形成的变化以及社会互动。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Comparison of Coherent and Incoherent Donut Beams for Deep Tissue STED Microscopy

• DOI: 10.1364/cleo_si.2023.sm1i.2
• 发表时间: 2023-05
• 期刊: 2023 Conference on Lasers and Electro-Optics (CLEO)
• 作者: Thariq Shanavas;R. McLeod;M. Siemens;J. Gopinath

OpenSTED: Inexpensive and open-source implementation of Dynamic Intensity Minimum (DyMIN) for Stimulated Emission Depletion (STED) microscopy

OpenSTED：用于受激发射损耗 (STED) 显微镜的动态强度最小值 (DyMIN) 的廉价开源实施

• DOI: 10.1364/ntm.2021.nw4c.3
• 发表时间: 2021
• 期刊: Biophotonics Congress 2021
• 作者: Meyer, Stephanie A.;Siemens, Mark E.;Gopinath, Juliet T.;Restrepo, Diego;Gibson, Emily A.
• 通讯作者: Gibson, Emily A.

Two-photon, fiber-coupled, super-resolution microscope for biological imaging

• DOI: 10.1063/5.0075012
• 发表时间: 2022-03-01
• 期刊: APL PHOTONICS
• 影响因子: 5.6
• 作者: Heffernan，Brendan M.;Riley，Peter S.;Gopinath，Juliet T.
• 通讯作者: Gopinath，Juliet T.

Two-photon Fiber STED Microscope Using Polarization Maintaining Fiber

使用保偏光纤的双光子光纤 STED 显微镜

• DOI: 10.1364/cleo_si.2021.sw2d.2
• 发表时间: 2021
• 期刊: Conference on Lasers and Electro-Optics
• 作者: Heffernan, Brendan M.;Riley, Peter;Supekar, Omkar D.;Meyer, Stephanie A.;George, Nicholas M.;Restrepo, Diego;Siemens, Mark E.;Gibson, Emily A.;Gopinath, Juliet T.
• 通讯作者: Gopinath, Juliet T.