MRI-Consortium: Acquisition of an advanced mass spectrometer

MRI 联盟：购买先进的质谱仪

• 批准号: 1920268
• 负责人: Robert Moritz
• 金额: $ 77万
• 依托单位: Institute for Systems Biology
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-01 至 2022-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1920268&HistoricalAwards=false
• 关键词: MRI; Consortium; Acquisition; advanced; mass

An award is made to the Institute for Systems Biology (ISB) to support the purchase of an advanced mass spectrometry (MS) instrument. Mass spectrometry is used by researchers to catalogue and quantify many variants of the protein complement of an organism or organelle system and to measure other metabolites. Researchers at the ISB will extend their previous work with advanced detectors, software and lab protocols to improve protein and metabolite profiling in biological samples. Through training of researchers directly or attending expert courses given regularly at ISB, this project will enhance the teaching, training and learning by providing innovative tools to maintain the cutting-edge curriculum of these courses from PhD students, post-docs to experienced PIs. The ISB award-winning high school education program will also support inquiry-based teaching practices at the grassroots. Students and teachers of underrepresented groups will collaborate with scientists to bring current science concepts and practices into high school classrooms. The new MS instrument will provide enhanced capabilities of comprehensive high-throughput discovery and targeted analyses, characterization of post-translational modifications, and protein interaction analysis. Using knowledge gained from previous research efforts, as well as computational tools for prediction and spectral interpretation, the ISB researchers will provide new insights into multiple biochemical events such as transcription/translation interplay, organelle biogenesis, metabolic network analysis, novel capture and quantitation reagent development and their applications. The new system will complement the computational expertise in proteomics hardware and software development capabilities among ISB researchers to advance many other projects. The use of data independent analysis (DIA) with this instrument and interrogation through new software tools for data analysis will provide users unprecedented capabilities in molecular ion identification and quantitation. Results from the studies will be published in peer-reviewed journals and presented at regional and national scientific meetings.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

授予系统生物学研究所（ISB）以支持购买先进的质谱仪（MS）。 研究人员使用质谱法对生物体或细胞器系统的蛋白质补体的许多变体进行分类和定量，并测量其他代谢物。 ISB的研究人员将利用先进的检测器、软件和实验室协议来扩展他们以前的工作，以改善生物样品中的蛋白质和代谢物分析。 通过直接培训研究人员或参加ISB定期提供的专家课程，该项目将通过提供创新工具来加强教学，培训和学习，以保持这些课程的前沿课程，从博士生，博士后到经验丰富的PI。ISB获奖的高中教育计划还将支持基层的探究式教学实践。代表性不足群体的学生和教师将与科学家合作，将当前的科学概念和实践带入高中课堂。 新的MS仪器将提供全面的高通量发现和靶向分析，翻译后修饰表征和蛋白质相互作用分析的增强功能。利用从以前的研究工作中获得的知识，以及用于预测和光谱解释的计算工具，ISB研究人员将提供对多种生物化学事件的新见解，如转录/翻译相互作用，细胞器生物发生，代谢网络分析，新型捕获和定量试剂开发及其应用。新系统将补充ISB研究人员在蛋白质组学硬件和软件开发能力方面的计算专业知识，以推进许多其他项目。 使用该仪器进行数据独立分析（DIA），并通过新的数据分析软件工具进行询问，将为用户提供前所未有的分子离子鉴定和定量能力。研究结果将发表在同行评审的期刊上，并在地区和国家科学会议上发表。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Engineered SH2 Domains for Targeted Phosphoproteomics.

• DOI: 10.1021/acschembio.2c00051
• 发表时间: 2022-06-17
• 期刊: ACS CHEMICAL BIOLOGY
• 影响因子: 4
• 作者: Martyn, Gregory D.;Veggiani, Gianluca;Kusebauch, Ulrike;Morrone, Seamus R.;Yates, Bradley P.;Singer, Alex U.;Tong, Jiefei;Manczyk, Noah;Gish, Gerald;Sun, Zhi;Kurinov, Igor;Sicheri, Frank;Moran, Michael F.;Moritz, Robert L.;Sidhu, Sachdev S.
• 通讯作者: Sidhu, Sachdev S.

Improved Analysis of Cross-Linking Mass Spectrometry Data with Kojak 2.0, Advanced by Integration into the Trans-Proteomic Pipeline

通过集成到跨蛋白质组管道中，利用 Kojak 2.0 改进了交联质谱数据的分析

• DOI: 10.1021/acs.jproteome.2c00670
• 发表时间: 2023
• 期刊: Journal of Proteome Research
• 影响因子: 4.4
• 作者: Hoopmann, Michael R.;Shteynberg, David D.;Zelter, Alex;Riffle, Michael;Lyon, Andrew S.;Agard, David A.;Luan, Qing;Nolen, Brad J.;MacCoss, Michael J.;Davis, Trisha N.
• 通讯作者: Davis, Trisha N.

The Crux Toolkit for Analysis of Bottom-Up Tandem Mass Spectrometry Proteomics Data.

• DOI: 10.1021/acs.jproteome.2c00615
• 发表时间: 2023-02-03
• 期刊: JOURNAL OF PROTEOME RESEARCH
• 影响因子: 4.4
• 作者: Kertesz-Farkas, Attila;Acquaye, Frank Lawrence Nii Adoquaye;Bhimani, Kishankumar;Eng, Jimmy K.;Fondrie, William E.;Grant, Charles;Hoopmann, Michael R.;Lin, Andy;Lu, Yang Y.;Moritz, Robert L.;MacCoss, Michael J.;Noble, William Stafford
• 通讯作者: Noble, William Stafford

Plasmodium falciparum Calcium-Dependent Protein Kinase 4 is Critical for Male Gametogenesis and Transmission to the Mosquito Vector.

• DOI: 10.1128/mbio.02575-21
• 发表时间: 2021-12-21
• 期刊: mBio
• 影响因子: 6.4
• 作者: Kumar S;Haile MT;Hoopmann MR;Tran LT;Michaels SA;Morrone SR;Ojo KK;Reynolds LM;Kusebauch U;Vaughan AM;Moritz RL;Kappe SHI;Swearingen KE
• 通讯作者: Swearingen KE