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MRI Consortium: Acquisition of a Leica TCS SP8 STED 3X Super Resolution microscope

MRI 联盟：购买 Leica TCS SP8 STED 3X 超分辨率显微镜

基本信息

批准号：
1725984
负责人：
Chaitanya Ullal
金额：
$ 103.06万
依托单位：
Rensselaer Polytechnic Institute
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2017
资助国家：
美国
起止时间：
2017-09-01 至 2020-08-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1725984&HistoricalAwards=false
关键词：
MRI Consortium Acquisition Leica TCS

项目摘要

An award is made to Rensselaer Polytechnic Institute (RPI) to purchase a super-resolution microscope (Leica STED) that will serve as the super-resolution center in the New York Capital Region. This project will impact many layers of our society. First, information about STED microscopy and its applications will be incorporated in several courses (Methods in Biophysics BCBP4800-RPI; Bio513 Modern Use of Light Microscopy-SUNY; Bioimaging AMC 608; Learn, Design, Teach Nanotechnology RPI MTLE 4960; Microscopy and Analysis "Tools"-Wadsworth Center). It will be part of the CBIS Annual Hands-On Workshops. It will be part of the Biological Sciences Seminar Series at all four institutions. Second, it will be incorporated in community outreach programs to encourage students into STEM fields: Black Family Technology Awareness Day; the High School Summer Research Program and the LSAMP Summer Research Program; the annual Science, Technology, Arts, and Architecture at Rensselaer Program that reaches out to talented underrepresented minorities and young women. Third, information about this technology and the research that it enables, including in nontraditional fields, will be distributed throughout the region's biotechnology community via Bioconnex, a network dedicated to the development of partnerships between research, education and business in the NY Capital region. Fourth, one of the RPI "Public Interest Research Forums" will be dedicated to STED and will establish the connection between this cutting-edge technology, the Nobel prize and implications to the general public.The intellectual merit of this project is the research activities that are enabled through the application of simulated emission depletion (STED) microscopy. STED microscopy creates super-resolution images by the selective deactivation of fluorophores, minimizing the area of illumination, and thus, bypassing the diffraction limit of conventional light microscopy. The microscope will enable very interdisciplinary research: 25 investigators across four institutions are strongly supporting this application, representing fourteen different Departments/Centers. Areas of research enabled include the study of cellular adherens and cellular permeability, STEM cell research, smooth muscle cell development, intracellular molecular interactions, endothelial cell development, and functional characterization of presynaptic terminals at small central synapses. The critical need for this technology and the diverse research applications for the instrument demonstrate that the super resolution microscope will become an extraordinary regional resource. Knowledge accumulated as a result of using STED imaging will have a tremendous positive impact in the fields of biological sciences, materials characterization and tissue engineering in the greater New York Capital Region.

Rensselaer Polytechnic Institute（RPI）获得了一个购买超分辨率显微镜（Leica STED）的奖项，该显微镜将作为纽约首都地区的超分辨率中心。这个项目将影响我们社会的许多层面。首先，有关STED显微镜及其应用的信息将被纳入几门课程（生物物理学方法BCBP 4800-RPI; Bio 513现代光学显微镜的使用-SUNY;生物成像AMC 608;学习，设计，教授纳米技术RPI MTLE 4960;显微镜和分析“工具”-沃兹沃斯中心）。这将是CBIS年度实践研讨会的一部分。这将是所有四个机构的生物科学研讨会系列的一部分。其次，它将被纳入社区外展计划，以鼓励学生进入STEM领域：黑人家庭技术意识日;高中暑期研究计划和LSAMP暑期研究计划;伦斯勒年度科学，技术，艺术和建筑计划，该计划面向有才华的代表性不足的少数民族和年轻女性。第三，将通过Bioconnex在整个区域的生物技术界传播关于这项技术及其所促成的研究，包括在非传统领域的研究的信息，Bioconnex是一个致力于在纽约首都地区发展研究、教育和商业伙伴关系的网络。第四，RPI的“公共利益研究论坛”将致力于STED，并将建立这种尖端技术，诺贝尔奖和对公众的影响之间的联系。该项目的智力价值是通过模拟发射耗尽（STED）显微镜的应用实现的研究活动。STED显微镜通过选择性地使荧光团失活来创建超分辨率图像，最大限度地减少照明面积，从而绕过传统光学显微镜的衍射极限。显微镜将使非常跨学科的研究：25个研究人员在四个机构大力支持这一应用，代表14个不同的部门/中心。研究领域包括细胞粘附和细胞渗透性的研究，干细胞研究，平滑肌细胞发育，细胞内分子相互作用，内皮细胞发育和小中央突触突触前末梢的功能表征。对这项技术的迫切需求和仪器的各种研究应用表明，超分辨率显微镜将成为一种非凡的区域资源。由于使用STED成像而积累的知识将在大纽约首都地区的生物科学、材料表征和组织工程领域产生巨大的积极影响。

项目成果

期刊论文数量（10）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Super-Resolution Imaging of Spatial Heterogeneities in Model Thermosensitive Hydrogels with Implications for Their Origins

热敏水凝胶模型中空间异质性的超分辨率成像及其起源的意义

DOI：
10.1021/acs.macromol.1c00754
发表时间：
2021
期刊：
Macromolecules
影响因子：
5.5
作者：
Kenath, Gopal S.;Karanastasis, Apostolos A.;Ullal, Chaitanya K.
通讯作者：
Ullal, Chaitanya K.

Quantification of functional crosslinker reaction kinetics via super-resolution microscopy of swollen microgels

通过溶胀微凝胶的超分辨率显微镜定量功能交联剂反应动力学

DOI：
10.1039/c9sm01618j
发表时间：
2019
期刊：
Soft Matter
影响因子：
3.4
作者：
Karanastasis, Apostolos A.;Kenath, Gopal S.;Sundararaman, Ravishankar;Ullal, Chaitanya K.
通讯作者：
Ullal, Chaitanya K.

Rapid fabrication of collagen bundles mimicking tumor-associated collagen architectures