Collaborative Research: Cestode phylogeny and genomics

合作研究：绦虫系统发育和基因组学

• 批准号: 1921411
• 负责人: Kirsten Jensen
• 金额: $ 28.68万
• 依托单位: University of Kansas Center for Research Inc
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1921411&HistoricalAwards=false
• 关键词: Collaborative; Research; Cestode; phylogeny; genomics

Tapeworms are parasites that include numerous species of medial and veterinary importance in mammals as well as thousands of species that parasitize essentially all other groups of vertebrate animals. Yet, we know little about the evolution of the major groups of tapeworms. Not only does this hamper our ability to treat tapeworm infections of medical and veterinary importance, but it also limits the use of tapeworms as model parasite systems to help expand our understanding of food web connections and ecosystem health, and to help inform fisheries management practices. This project aims to generate the molecular and morphological data required to substantially expand our understanding of the evolution of tapeworms. In doing so, it will transform tapeworms and their vertebrate hosts into one of the best known host/parasite systems globally, allowing this system to reach its full potential. The training provided to undergraduate, graduate and postdoctoral researchers will equip them with transferable STEM skills. Furthermore, information about tapeworms will be made publicly available on-line to diverse audiences of all ages by creating a key to the major tapeworm groups, developing an e-book version of a children's book on tapeworms, and building upon an existing global cestode database. A recent global survey has identified tapeworm lineages hosted by sharks and rays as central to the diversification of the Cestoda. This class currently comprises 19 orders. Preliminary phylogenetic work suggests that the current ordinal classification of the class substantially underestimates lineage diversity, especially within the groups of elasmobranch-hosted cestodes. The proposed work aims to provide a robust phylogeny of the class based on targeted gene capture of 738 loci for 960 species of tapeworms. Cestode higher-level classification will be revised to reflect monophyletic groups, informed by new morphological and molecular data. The generation of complete genomes for 20 species spanning the approximately 200-million year gap between the cestodes of medical and veterinary importance for which such data are currently available will provide insight into patterns of taxon-specific gene family evolution across cestodes. Genomic analyses have been designed to take advantage of the multiple independent transitions between marine and non-marine habitats, elasmobranch and non-elasmobranch definitive hosts, and invertebrate and vertebrate final intermediate hosts that have occurred over evolutionary time to identify signatures of parallel evolution. Such signatures are anticipated to provide valuable insight into environmental factors that may have played a role in the evolution of parasite systems with complex life cycles.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

绦虫是一种寄生虫，包括许多在哺乳动物中具有医学和兽医重要性的物种，以及寄生在几乎所有其他脊椎动物群体中的数千种物种。然而，我们对绦虫主要类群的进化知之甚少。这不仅妨碍了我们治疗具有医学和兽医重要性的绦虫感染的能力，而且还限制了绦虫作为模型寄生虫系统的使用，以帮助扩大我们对食物网连接和生态系统健康的了解，并帮助为渔业管理实践提供信息。该项目旨在生成大幅扩展我们对绦虫进化的理解所需的分子和形态学数据。在此过程中，它将把绦虫及其脊椎动物宿主转变为全球最著名的宿主/寄生虫系统之一，使该系统能够充分发挥其潜力。为本科生、研究生和博士后研究人员提供的培训将使他们具备可转移的 STEM 技能。此外，有关绦虫的信息将通过创建主要绦虫群体的密钥、开发有关绦虫的儿童读物的电子书版本以及在现有的全球绦虫数据库的基础上，在网上向所有年龄段的不同受众公开提供。最近的一项全球调查发现，鲨鱼和鳐鱼寄生的绦虫谱系是绦虫多样化的核心。该级别目前包括 19 个订单。初步的系统发育工作表明，目前该类的顺序分类大大低估了谱系多样性，特别是在软骨鱼寄生的绦虫群体中。拟议的工作旨在基于对 960 种绦虫的 738 个基因座的靶向基因捕获，提供该类别的强大系统发育。绦虫的高级分类将根据新的形态学和分子数据进行修订，以反映单系类群。 20个物种的完整基因组的产生跨越了医学和兽医重要性的绦虫之间大约2亿年的差距，目前可以获得这些数据，这将有助于深入了解绦虫中分类单元特异性基因家族进化的模式。基因组分析旨在利用进化过程中发生的海洋和非海洋栖息地、软骨鱼类和非软骨鱼类最终宿主以及无脊椎动物和脊椎动物最终中间宿主之间的多重独立转变来识别平行进化的特征。这些签名预计将为了解可能在具有复杂生命周期的寄生虫系统的进化中发挥作用的环境因素提供有价值的见解。该奖项反映了 NSF 的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Expanding known global biodiversity of Yamaguticestus (Cestoda: Phyllobothriidea) parasitizing catsharks (Pentanchidae and Scyliorhinidae)

扩大寄生猫鲨（Pentanchidae 和 Scylorhinidae）的 Yamaguticestus（Cestoda：Phyllobothriidea）已知的全球生物多样性

• DOI: 10.1080/14772000.2021.1946617
• 发表时间: 2021
• 期刊: Systematics and Biodiversity
• 影响因子: 1.9
• 作者: Caira, J. N.;Pickering, M.;Jensen, K.
• 通讯作者: Jensen, K.

Three new species of Duplicibothrium (Cestoda: ‘Tetraphyllidea’) from cownose rays in Senegal with a phylogenetic analysis of the genus

塞内加尔牛鼻鳐的三个新种 Duplicibothrium（Cestoda：Tetraphyllidea）及其属的系统发育分析

• DOI: 10.1017/s0022149x21000766
• 发表时间: 2022
• 期刊: Journal of Helminthology
• 影响因子: 1.6
• 作者: Stephan, Douglas;Caira, Janine N.
• 通讯作者: Caira, Janine N.

Four New Species of Paraorygmatobothrium (Eucestoda: Phyllobothriidea) from Sharks of the Gulf of Mexico and the Atlantic Ocean, with Comments on Their Host Specificity

墨西哥湾和大西洋鲨鱼的四种新种 Paraorygmatobothrium（Eucestoda：Phyllobothriidea）及其寄主特异性

• DOI: 10.1645/19-129
• 发表时间: 2020
• 期刊: Journal of Parasitology
• 影响因子: 1.3
• 作者: Ruhnke, T. R.;Daniel, V.;Jensen, K.
• 通讯作者: Jensen, K.

Emerging global novelty in phyllobothriidean tapeworms (Cestoda: Phyllobothriidea) from sharks and skates (Elasmobranchii)

• DOI: 10.1093/zoolinnean/zlaa185
• 发表时间: 2021-02-17
• 期刊: ZOOLOGICAL JOURNAL OF THE LINNEAN SOCIETY
• 影响因子: 2.8
• 作者: Caira, Janine N.;Bueno, Veronica;Jensen, Kirsten
• 通讯作者: Jensen, Kirsten

Three new species of 'tetraphyllidean' cestodes from an undescribed bamboo shark (Orectolobiformes: Hemiscylliidae) in Sri Lanka

来自斯里兰卡未描述的竹鲨（直齿目：Hemiscylliidae）的三种新种“四叶齿”绦虫

• DOI: 10.14411/fp.2021.004
• 发表时间: 2021
• 期刊: Folia Parasitologica
• 影响因子: 1.6
• 作者: Caira, Janine N.;Otto, Kyle;Fernando, Daniel;Jensen, Kirsten
• 通讯作者: Jensen, Kirsten