BBSRC-NSF/BIO: Collaborative Research: Focusing a quantitative lens on Synthetic Phototrophic Communities

BBSRC-NSF/BIO：合作研究：将定量视角聚焦于合成光养群落

• 批准号: 1921439
• 负责人: Seppe Kuehn
• 金额: $ 33.19万
• 依托单位: University of Illinois at Urbana-Champaign
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-08-01 至 2021-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1921439&HistoricalAwards=false
• 关键词: BBSRC; NSF; BIO; Collaborative; Research

Microbial communities populate nearly all environments on our planet. These environments include the human gut, the surface of corals in the oceans, and plant roots in the soil. In these communities, bacteria interact with each other to sustain environmental health. Understanding these interactions will reveal factors that establish robust, productive communities. This project will focus on bacteria isolated from hot spring mats of Yellowstone National Park. A deeper understanding of the physical and metabolic conversations among bacteria that thrive in this extreme environment will be gained and used to model these interactions. New courses on microbial consortia will be developed and shared by all participating Universities. The PIs will also develop programs in partnership with local science museums and TV stations to encourage science literacy and community involvement. This project is focused on extremophile bacteria isolated from the hot spring mats of Yellowstone National Park. The approach will be to query the entire bacterial community within the mat as well as synthetic combinations of the bacteria grown under defined conditions in the laboratory. The application of sequencing and metabolomic technologies will reveal key metabolic pathways among members of the community under different light and nutrient conditions and over the diel cycle. The work will evaluate the hypothesis that microbial communities act like an integrated machine rather than as individual species grown under axenic conditions in the laboratory, and that this integration maintains functionalities critical for life in natural habitats. Experiments will also be performed to analyze how abrupt changes in light intensity, temperature and microbial composition impact mat growth, structure, electrical and metabolic networks.This collaborative US/UK project is supported by the US National Science Foundation and the UK Biotechnology and Biological Sciences Research CouncilThis award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

微生物群落几乎存在于地球上的所有环境中。这些环境包括人类的肠道、海洋中的珊瑚表面和土壤中的植物根。在这些社区中，细菌相互作用以维持环境健康。了解这些相互作用将揭示建立强大、高效的社区的因素。该项目将专注于从黄石国家公园的温泉垫子中分离出细菌。我们将更深入地了解在这种极端环境中茁壮成长的细菌之间的物理和代谢对话，并将其用于对这些相互作用进行建模。关于微生物协会的新课程将由所有参与的大学开发和分享。PIS还将与当地科学博物馆和电视台合作开发方案，以鼓励科学素养和社区参与。该项目的重点是从黄石国家公园的温泉垫子中分离出极端细菌。该方法将询问垫子内的整个细菌群落以及实验室中在特定条件下生长的细菌的合成组合。测序和代谢组学技术的应用将揭示群落成员在不同光照和营养条件下以及在日粮循环中的关键代谢途径。这项工作将评估这样一种假设，即微生物群落的行为像一个集成的机器，而不是作为在实验室无菌条件下生长的单个物种，并且这种集成保持了对自然栖息地中的生命至关重要的功能。还将进行实验，以分析光照强度、温度和微生物组成的突然变化如何影响垫子的生长、结构、电气和代谢网络。这一美英合作项目得到了美国国家科学基金会和英国生物技术和生物科学研究委员会的支持。该奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。