22-BBSRC/NSF-BIO: Community-dependent CRISPR-cas evolution and robust community function
22-BBSRC/NSF-BIO:群落依赖性 CRISPR-cas 进化和强大的群落功能
基本信息
- 批准号:BB/Y008774/1
- 负责人:
- 金额:$ 58.88万
- 依托单位:
- 依托单位国家:英国
- 项目类别:Research Grant
- 财政年份:2024
- 资助国家:英国
- 起止时间:2024 至 无数据
- 项目状态:未结题
- 来源:
- 关键词:
项目摘要
Rationally designed synthetic microbial communities offer an exciting avenue to decipher basic rules of microbial organization and engineer novel microbial solutions to pressing applied challenges. Yet, the robustness of synthetic microbiomes to environmental perturbations remains relatively untested. A major class of microbiome perturbation stems from assault by molecular parasites such as bacteriophage viruses (phages). Individual species commonly evolve resistance to phages by modifying or entirely deleting the surface receptor used by the phage, but this can have substantial impacts on the functional capacities and species interactions of the bacterium, due to the importance of surface factors in mediating environmental interactions. In a synthetic community perspective, evolved surface factor modifications in response to phage exposure risk damaging the functional capacities of the community. Bacteria can also evolve resistance to phages via their 'adaptive immunity' mechanism, known as CRISPR-Cas, which leave the functional capacity of the cell intact, yet this pathway of acquired resistance is rarely seen in a lab setting. The paucity of lab CRISPR-Cas evolution (lack of spacer acquisition in response to phage exposure) presents a challenge to our understanding of CRISPR-Cas as a primary mechanism of acquired resistance. We hypothesize that CRISPR-Cas immunity acquisition is an emergent property of intra- and inter-specific cell-cell signaling mechanisms (Aim 1) and community-dependent fitness costs (Aim 2), which together promote robust community functioning. We further hypothesize that the regulatory (Aim 1) and eco-evolutionary (Aim 2) impacts of phage exposure on community performance are predictable, given information on species interactions and available mechanisms of phage resistance.
合理设计的合成微生物群落为破译微生物组织的基本规则和设计新的微生物解决方案来解决紧迫的应用挑战提供了一条令人兴奋的途径。然而,合成微生物组对环境扰动的稳健性仍然相对未经测试。一类主要的微生物群扰动源于分子寄生虫,如噬菌体病毒(噬菌体)的攻击。单个物种通常通过修饰或完全删除噬菌体使用的表面受体来进化对噬菌体的抗性,但由于表面因子在介导环境相互作用中的重要性,这可能对细菌的功能能力和物种相互作用产生重大影响。从合成群落的角度来看,进化的表面因子修饰是为了应对噬菌体暴露的风险,从而损害群落的功能能力。细菌也可以通过它们的“适应性免疫”机制进化出对噬菌体的耐药性,这种机制被称为CRISPR-Cas,它使细胞的功能能力保持完整,但这种获得性耐药性的途径在实验室环境中很少见到。实验室CRISPR-Cas进化的缺乏(缺乏对噬菌体暴露的间隔获取)对我们将CRISPR-Cas作为获得性耐药的主要机制的理解提出了挑战。我们假设CRISPR-Cas免疫获得是细胞内和细胞间特异性信号机制(Aim 1)和社区依赖的适应度成本(Aim 2)的一个新特性,它们共同促进了强大的社区功能。我们进一步假设噬菌体暴露对群落性能的调节(目标1)和生态进化(目标2)影响是可预测的,已知物种相互作用的信息和噬菌体抗性的可用机制。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Edze Rients Westra其他文献
Edze Rients Westra的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Edze Rients Westra', 18)}}的其他基金
Multi-layered bacterial genome defences: linking molecular mechanisms to bacteria-MGE conflicts in single cells, populations, and communities.
多层细菌基因组防御:将分子机制与单细胞、群体和群落中的细菌-MGE 冲突联系起来。
- 批准号:
BB/X003051/1 - 财政年份:2023
- 资助金额:
$ 58.88万 - 项目类别:
Research Grant
COMMUNICATE: Understanding the evolution and ecology of viral communication
沟通:了解病毒式传播的进化和生态
- 批准号:
EP/X030377/1 - 财政年份:2023
- 资助金额:
$ 58.88万 - 项目类别:
Research Grant
The impact of spatial structure of CRISPR-phage coevolution
CRISPR-噬菌体协同进化空间结构的影响
- 批准号:
NE/S001921/1 - 财政年份:2019
- 资助金额:
$ 58.88万 - 项目类别:
Research Grant
Identifying factors that drive CRISPR-Cas-dependent phage resistance in bacteria
识别细菌中 CRISPR-Cas 依赖性噬菌体抗性的驱动因素
- 批准号:
BB/N017412/1 - 财政年份:2017
- 资助金额:
$ 58.88万 - 项目类别:
Research Grant
Identifying ecological factors that drive the evolution of innate versus adaptive immunity in bacteria
识别驱动细菌先天免疫和适应性免疫进化的生态因素
- 批准号:
NE/M018350/1 - 财政年份:2015
- 资助金额:
$ 58.88万 - 项目类别:
Fellowship
相似海外基金
BBSRC-NSF/BIO: An AI-based domain classification platform for 200 million 3D-models of proteins to reveal protein evolution
BBSRC-NSF/BIO:基于人工智能的域分类平台,可用于 2 亿个蛋白质 3D 模型,以揭示蛋白质进化
- 批准号:
BB/Y000455/1 - 财政年份:2024
- 资助金额:
$ 58.88万 - 项目类别:
Research Grant
BBSRC-NSF/BIO: An AI-based domain classification platform for 200 million 3D-models of proteins to reveal protein evolution
BBSRC-NSF/BIO:基于人工智能的域分类平台,可用于 2 亿个蛋白质 3D 模型,以揭示蛋白质进化
- 批准号:
BB/Y001117/1 - 财政年份:2024
- 资助金额:
$ 58.88万 - 项目类别:
Research Grant
22-BBSRC/NSF-BIO Building synthetic regulatory units to understand the complexity of mammalian gene expression
22-BBSRC/NSF-BIO 构建合成调控单元以了解哺乳动物基因表达的复杂性
- 批准号:
BB/Y008898/1 - 财政年份:2024
- 资助金额:
$ 58.88万 - 项目类别:
Research Grant
20-BBSRC/NSF-BIO Regulatory control of innate immune response in marine invertebrates
20-BBSRC/NSF-BIO 海洋无脊椎动物先天免疫反应的调节控制
- 批准号:
BB/W017865/1 - 财政年份:2024
- 资助金额:
$ 58.88万 - 项目类别:
Research Grant
22-BBSRC/NSF-BIO - Interpretable & Noise-robust Machine Learning for Neurophysiology
22-BBSRC/NSF-BIO - 可解释
- 批准号:
BB/Y008758/1 - 财政年份:2024
- 资助金额:
$ 58.88万 - 项目类别:
Research Grant
UKRI/BBSRC-NSF/BIO: Interpretable and Noise-Robust Machine Learning for Neurophysiology
UKRI/BBSRC-NSF/BIO:用于神经生理学的可解释且抗噪声的机器学习
- 批准号:
2321840 - 财政年份:2023
- 资助金额:
$ 58.88万 - 项目类别:
Continuing Grant
UKRI/BBSRC-NSF/BIO:Hidden costs of infection: mechanisms by which parasites disrupt host-microbe symbioses and alter development
UKRI/BBSRC-NSF/BIO:感染的隐性成本:寄生虫破坏宿主-微生物共生并改变发育的机制
- 批准号:
2322173 - 财政年份:2023
- 资助金额:
$ 58.88万 - 项目类别:
Continuing Grant
21-BBSRC/NSF-BIO: Developing large serine integrases as tools for constructing and manipulating synthetic replicons.
21-BBSRC/NSF-BIO:开发大型丝氨酸整合酶作为构建和操作合成复制子的工具。
- 批准号:
BB/X012085/1 - 财政年份:2023
- 资助金额:
$ 58.88万 - 项目类别:
Research Grant
UKRI/BBSRC-NSF/BIO Determining the Roles of Fusarium Effector Proteases in Plant Pathogenesis
UKRI/BBSRC-NSF/BIO 确定镰刀菌效应蛋白酶在植物发病机制中的作用
- 批准号:
BB/X012131/1 - 财政年份:2023
- 资助金额:
$ 58.88万 - 项目类别:
Research Grant
BBSRC-NSF/BIO. Globally harmonized re-analysis of Data Independent Acquisition (DIA) proteomics datasets enables the creation of new resources
BBSRC-NSF/BIO。
- 批准号:
BB/X002020/1 - 财政年份:2023
- 资助金额:
$ 58.88万 - 项目类别:
Research Grant