I-Corps: Multielectrode Array Biosensor

I-Corps项目的广泛影响和商业潜力最终将使研究人员更好地了解大脑，提高神经学研究的生产力。这项发明的社会影响将帮助研究人员、科学家和医生在大脑中进行精确的神经化学测量，以更好地理解复杂的行为、疾病和药物状态。碳多电极阵列技术将使大脑测量能够更好地了解大脑异质性和神经系统疾病，如成瘾/药物滥用、癫痫、抑郁和焦虑，这些疾病给我们的社会带来负担。这些多电极阵列将同时对大脑多个区域的神经递质进行复杂的测量。这项新技术的商业潜力是巨大的，因为目前还没有现有的设备可以同时测量大脑多个区域的神经传递。这些益处对于政府、学术、研究机构和行业的研究人员、临床医生和研究人员都是有价值的。I-Corps项目将使用碳涂层金属线和碳纤维作为电极材料来设计、开发和制造多电极阵列。这些多电极阵列将被用作电极材料，用于同时检测大脑多个区域的神经递质，如多巴胺、血清素、去甲肾上腺素等。目前还没有其他神经系统设备将碳纤维多电极阵列用作生物传感器，因此这项技术是最先进的。本研究将利用快速扫描循环伏安法（FSCV）检测临床前模型中的神经递质。在动物模型中，电极材料将被施加一个变化的电压来测量体内神经化学变化。该项目所依据的技术成果来自于进一步发展这些多电极阵列的实验室实验。碳纤维多电极阵列在实验室中浸泡在制备好的神经递质原液中，并使用FSCV和数据分析软件成功测量了多巴胺和其他神经递质。该奖项反映了美国国家科学基金会的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

The broader impact/commercial potential of this I-Corps project ultimately will allow researchers to better understand the brain and improve the productivity of neurological studies. The societal impact of this invention will help researchers, scientists, and physicians alike make precise neurochemical measurements in the brain to better understand complex behaviors, diseases, and drug states. Carbon multielectrode arrays technology will enable measurements in the brain to better understand brain heterogeneity and neurological diseases that burden our society such as addiction/drug abuse, epilepsy, depression and anxiety. These multielectrode arrays will perform complex measurements of neurotransmitters in multiple brain regions simultaneously. The commercial potential for this novel technology is substantial in that, currently, there is no existing device that can simultaneously measure neurotransmission in multiple brain regions. These benefits should be of value to investigators, clinicians, and researchers in government, academic, and research institutions as well as industry.This I-Corps project will use carbon coated metal wires and carbon fibers as electrode materials to design, develop, and engineer multielectrode arrays. These multielectrode arrays will be used as electrode materials to detect neurotransmitters such as dopamine, serotonin, norepinephrine, and others in multiple brain regions simultaneously. This technology is cutting edge as no other neurological device has been developed with carbon fiber multielectrode arrays to be utilized as biosensors. This research will utilize fast scan cyclic voltammetry (FSCV) to detect neurotransmitters in preclinical models. A changing voltage will be applied to the electrode material to measure neurochemical changes in vivo in animal models. The technical results on which the project is based arise from laboratory experiments that have further developed these multielectrode arrays. The carbon fiber multielectrode arrays have been immersed in prepared neurotransmitter stock solutions in the laboratory and have successfully measured dopamine and other neurotransmitters using FSCV and data analysis software.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.