Flexible and Transparent Multielectrode Arrays to Study Alzheimer's Pathology in Advanced In-Vitro Disease Models

灵活透明的多电极阵列用于在先进的体外疾病模型中研究阿尔茨海默病病理学

• 批准号: 2873651
• 金额: --
• 依托单位: University of Cambridge
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2873651
• 关键词: Flexible; Transparent; Multielectrode; Arrays; Study

Our lack of insights into neurodegenerative disorders, coupled with a projected increase in these disorders potentially increasing the load on the healthcare system (over 150 million people worldwide with dementia by 2050) has resulted in a rising sense of urgency towards conducting efforts to understand them. The differences between animal and humanphysiology have shifted the research efforts towards novel disease models, such as brain organoids based on induced pluripotent stem cells (iPSC). Due to their reduced dimensions, multielectrode arrays (MEA) are designed to enable detailed characterizations with a good spatial and temporal resolution. PEDOT: PSS MEAs show promising results dueto their biocompatibility, high conductivity and easy to design nature. This project aims at imparting characteristics like flexibility and transparency to traditional MEAs to permit their combination with high-resolution microscopy and enable the study of brain organoids using accurate electrophysiological as well as optical measurements. To minimise potentialnegative effects onto the growing organoid induced by its complex interaction with the polymer-based MEA, the proposed arrays will provide high porosity which ensures that the organoids achieve the necessary nutrition exchange through air-liquid interfaces helping them grow without any issues. Further measurements can then be carried out tomeasure the cellular interactions taking place during neurodegenerative pathology.

我们对神经退行性疾病缺乏了解，再加上预计这些疾病的增加可能会增加医疗保健系统的负担（到2050年，全球将有超过1.5亿人患有痴呆症），这使得人们越来越迫切地需要努力了解这些疾病。动物和人类生理学之间的差异已经将研究工作转向新的疾病模型，例如基于诱导多能干细胞（iPSC）的脑类器官。由于其尺寸减小，多电极阵列（MEA）被设计为具有良好的空间和时间分辨率的详细表征。PEDOT: PSS MEAs具有生物相容性好、电导率高、易于设计等优点，具有广阔的应用前景。该项目旨在为传统mea赋予灵活性和透明度等特性，使其能够与高分辨率显微镜相结合，并能够使用精确的电生理和光学测量来研究脑类器官。为了最大限度地减少由于与基于聚合物的MEA的复杂相互作用而对生长中的类器官产生的潜在负面影响，所提出的阵列将提供高孔隙度，确保类器官通过气液界面实现必要的营养交换，从而帮助它们毫无问题地生长。然后可以进行进一步的测量，以测量神经退行性病理过程中发生的细胞相互作用。