CAREER: Developmental origins of the shell gland and genetic networks for biomineralization in the mollusk Crepidula atrasolea
职业:软体动物 Crepidula atrasolea 的壳腺发育起源和生物矿化遗传网络
基本信息
- 批准号:1943606
- 负责人:
- 金额:$ 100万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Continuing Grant
- 财政年份:2021
- 资助国家:美国
- 起止时间:2021-03-15 至 2026-02-28
- 项目状态:未结题
- 来源:
- 关键词:
项目摘要
Biomineralization is the process by which living organisms produce minerals. Although it occurs in every kingdom of life, one of the most familiar and beautiful examples is the snail shell. The diversification of the snail shell is thought to be related to the abundance and success of this group of animals (the second largest phylum in terms of species number, after arthropods). With ocean temperatures increasing worldwide, there is also an urgent need to understand how shells form and grow to protect aquaculture species like oysters, clams, and mussels. Shells begin forming during development, when shell gland cells express a suite of transcription factor proteins that in turn regulate shell matrix proteins; matrix proteins form a scaffold that controls calcium carbonate crystalization. This project examines shell matrix formation using a well-studied marine snail, Crepidula. Taking advantage of tools to modify the genome of this species, this project examines which specific transcription factors control matrix protein production, and how they influence shell structure and growth. These studies will be the first of their kind among molluscs. Another innovative aspect of this project is that matrix proteins will be studied in the living animal. The research will be brought into the classroom as part of an inquiry-based laboratory course where undergraduates, graduate students, and high school students can study mollusc shell development and test their own hypotheses. Although much is known about the biomaterial properties, micro-structure, and evolution of molluscan shells, major questions remain about how they are made during the process of development. The overarching goal of this project is to make the first comprehensive study of the expression and function of extracellular matrix proteins embedded in the shell of the marine snail Crepidula atrasolea. Aim 1 will uncover the spatiotemporal expression patterns of shell gland-expressed regulatory genes and mantle tissue-expressed matrix genes, using scRNAseq, in situ hybridization, and proteomics. Aim 2 will use lineage tracing to show how the shell gland cells mature into the adult mantle tissue, and use CRISPR/Cas9 genome editing to make matrix protein-GFP fusions to study shell formation in vivo. Aim 3 will identify the upstream regulators of matrix proteins, and knock them down to test their function. This work will lead to the first bona fide gene regulatory network (GRN) for biomineralization in a mollusc. Aim 4 integrates this research into a new cell and developmental biology CURE lab class for undergraduates, graduate students, and high school students.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
生物矿化是生物体产生矿物质的过程。 虽然它发生在每一个生命王国,最熟悉和美丽的例子之一是蜗牛壳。 蜗牛壳的多样化被认为与这类动物的丰富和成功有关(就物种数量而言,这是仅次于节肢动物的第二大门)。 随着全球海洋温度的上升,迫切需要了解贝壳是如何形成和生长的,以保护牡蛎、蛤蜊和贻贝等水产养殖物种。 贝壳开始形成于发育过程中,此时贝壳腺细胞表达一套转录因子蛋白,这些转录因子蛋白反过来调节贝壳基质蛋白;基质蛋白形成控制碳酸钙结晶的支架。 本项目使用一种研究充分的海洋蜗牛Crepidula来研究壳基质的形成。 利用工具修改该物种的基因组,该项目研究了哪些特定的转录因子控制基质蛋白的产生,以及它们如何影响壳结构和生长。这些研究将是第一次在软体动物中进行。 该项目的另一个创新方面是将在活体动物中研究基质蛋白。 这项研究将作为基于探究的实验室课程的一部分带入课堂,本科生、研究生和高中生可以研究软体动物外壳的发育并测试他们自己的假设。 尽管人们对软体动物外壳的生物材料特性、微观结构和进化有了很多了解,但关于它们在发育过程中是如何形成的,仍然存在一些主要问题。 该项目的总体目标是首次全面研究嵌入海洋蜗牛Crepidula atrasolea外壳中的细胞外基质蛋白的表达和功能。 目的1利用scRNAseq、原位杂交和蛋白质组学技术,揭示贝壳腺表达的调控基因和外套膜组织表达的基质基因的时空表达模式。 目标2将使用谱系追踪来显示壳腺细胞如何成熟为成体外套膜组织,并使用CRISPR/Cas9基因组编辑来制备基质蛋白-GFP融合物,以研究体内壳的形成。 目标3将鉴定基质蛋白的上游调控因子,并敲除它们以测试它们的功能。 这项工作将导致第一个真正的基因调控网络(GRN)的生物矿化的软体动物。 目标4将这项研究整合到一个新的细胞和发育生物学CURE实验室课程中,面向本科生、研究生和高中生。该奖项反映了NSF的法定使命,并通过使用基金会的智力价值和更广泛的影响审查标准进行评估,被认为值得支持。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Deirdre Lyons其他文献
Engagement in Sport Career Transition Planning Enhances Performance: A Commentary
参与体育职业转型规划可提高绩效:评论
- DOI:
10.1080/15325024.2018.1516888 - 发表时间:
2019 - 期刊:
- 影响因子:4.7
- 作者:
Deirdre Lyons - 通讯作者:
Deirdre Lyons
How we Got Here: Perceived Influences on the Development and Success of International Athletes
我们如何走到这一步:对国际运动员发展和成功的影响
- DOI:
- 发表时间:
2006 - 期刊:
- 影响因子:0
- 作者:
P. Duffy;Deirdre Lyons;A. Moran;G. Warrington;C. Macmanus - 通讯作者:
C. Macmanus
Active surveillance of cervical intraepithelial neoplasia grade 2: 2025 British Society of Colposcopy and Cervical Pathology and European Society of Gynaecologic Oncology consensus statement
宫颈上皮内瘤变 2 级的主动监测:2025 年英国阴道镜检查和宫颈病理学会与欧洲妇科肿瘤学会共识声明
- DOI:
10.1016/s1470-2045(24)00524-2 - 发表时间:
2025-03-01 - 期刊:
- 影响因子:35.900
- 作者:
Maria Kyrgiou;Sarah J Bowden;Laura Burney Ellis;Anne Hammer;Deirdre Lyons;Theresa Freeman-Wang;Konstantinos S Kechagias;Ilkka Kalliala;Mario Preti;Vesna Kesic;Ignacio Zapardiel;Margaret Cruickshank;Murat Gultekin;Pierre Martin-Hirsch - 通讯作者:
Pierre Martin-Hirsch
Invasive cervical cancer following treatment of pre-invasive lesions: A potential theory based on a small case series
- DOI:
10.1016/j.ejogrb.2021.06.049 - 发表时间:
2021-09-01 - 期刊:
- 影响因子:
- 作者:
Evangelos Paraskevaidis;Antonios Athanasiou;Ilkka Kalliala;Anna Batistatou;Maria Paraskevaidi;Evripidis Bilirakis;Maria Nasioutziki;Minas Paschopoulos;Deirdre Lyons;Marc Arbyn;Margaret Cruickshank;Pierre Martin-Hirsch;Maria Kyrgiou - 通讯作者:
Maria Kyrgiou
Tackle Your Feelings: Experience of Help-Seeking for Mental Well-Being Concerns in Professional Rugby Union Players
解决你的感受:职业橄榄球联盟球员心理健康问题的求助经历
- DOI:
10.1123/jcsp.2022-0014 - 发表时间:
2022 - 期刊:
- 影响因子:1.9
- 作者:
Deirdre Lyons;Philip B. Clarke;R. Dempsey - 通讯作者:
R. Dempsey
Deirdre Lyons的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Deirdre Lyons', 18)}}的其他基金
The Developmental Biology of the Sea Urchin and Other Marine Invertebrates Meeting, Woods Hole, MA, April 15 - 19, 2020.
海胆和其他海洋无脊椎动物发育生物学会议,马萨诸塞州伍兹霍尔,2020 年 4 月 15 日至 19 日。
- 批准号:
1937613 - 财政年份:2020
- 资助金额:
$ 100万 - 项目类别:
Standard Grant
Spiralian White Paper Workshop; November 14-17, 2019; St. Augustine, Florida
螺旋白皮书研讨会;
- 批准号:
1937992 - 财政年份:2019
- 资助金额:
$ 100万 - 项目类别:
Standard Grant
相似海外基金
The developmental and evolutionary origins of vertebrate fins and limbs.
脊椎动物鳍和四肢的发育和进化起源。
- 批准号:
DP240102156 - 财政年份:2024
- 资助金额:
$ 100万 - 项目类别:
Discovery Projects
Collaborative Research: Developmental origins of emotional and epigenetic resilience among children
合作研究:儿童情绪和表观遗传复原力的发展起源
- 批准号:
2316775 - 财政年份:2023
- 资助金额:
$ 100万 - 项目类别:
Standard Grant
Developmental Origins of Aggressive and Impulsive Behavior
攻击性和冲动行为的发展起源
- 批准号:
10639422 - 财政年份:2023
- 资助金额:
$ 100万 - 项目类别:
Disentangling the prenatal role of fathers in the developmental origins of offspring's cardiometabolic disease
阐明父亲在后代心脏代谢疾病的发育起源中的产前作用
- 批准号:
481043 - 财政年份:2023
- 资助金额:
$ 100万 - 项目类别:
The Origins of Language and Musicality in Infant Crying: Integrating Deep Learning and Developmental Cognitive Neuroscience
婴儿哭泣中语言和音乐性的起源:深度学习和发展认知神经科学的结合
- 批准号:
23H01053 - 财政年份:2023
- 资助金额:
$ 100万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Collaborative Research: Developmental origins of emotional and epigenetic resilience among children
合作研究:儿童情绪和表观遗传复原力的发展起源
- 批准号:
2316774 - 财政年份:2023
- 资助金额:
$ 100万 - 项目类别:
Standard Grant
Developmental Origins of Cardiovascular Disease in Offspring from Non-Human Primate Pregnancies at Advanced Maternal Age
高龄非人类灵长类动物妊娠后代心血管疾病的发育起源
- 批准号:
10629732 - 财政年份:2023
- 资助金额:
$ 100万 - 项目类别:
Developmental cellular origins of pediatric medulloblastoma
儿童髓母细胞瘤的发育细胞起源
- 批准号:
488211 - 财政年份:2023
- 资助金额:
$ 100万 - 项目类别:
Operating Grants
Determining the Role of Fathers in the Developmental Origins of Cardiovascular Health
确定父亲在心血管健康发育起源中的作用
- 批准号:
471025 - 财政年份:2022
- 资助金额:
$ 100万 - 项目类别:
Operating Grants
Developmental origins and niches of the haematopoietic system
造血系统的发育起源和生态位
- 批准号:
MC_UU_00029/5 - 财政年份:2022
- 资助金额:
$ 100万 - 项目类别:
Intramural