SBIR Phase II: Low-Cost, High-Purity Biobased Glucaric Acid

SBIR II 期：低成本、高纯度生物基葡萄糖酸

• 批准号: 1951200
• 负责人: Darcy Prather
• 金额: $ 74.68万
• 依托单位: Kalion, Inc.
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-07-01 至 2024-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1951200&HistoricalAwards=false
• 关键词: SBIR; Phase; II; Low; Cost

The broader impact of this Small Business Technology Transfer (STTR) Phase II project is the low-cost, high-purity biobased production of glucaric acid, a compound with a broad range of applications. This production of glucaric acid will enable a broad change from petroleum-based sources for everyday materials, such as nylon in clothes or PET in two-liter bottles, to a bio-based product generated from renewable resources. Similarly, this technology will allow an evolution beyond the traditional phosphates used in water treatment systems to a safer, cost-effective alternative. The proposed project will develop a strain, fermentation process, and scalable downstream separation workflow to produce low-cost, high-purity glucaric acid from glucose as a feedstock. Microbial fermentation represents an attractive option for the production of fuels and valuable chemicals from renewable resources, such as cellulosic sugars. Microbes are well suited for the conversion of carbohydrate feedstocks; several examples of their metabolic engineering have been demonstrated to direct these feedstocks to non-natural chemicals and materials of industrial value, often as drop-in replacements for petroleum products. On the other hand, products derived from sugar oxidation pose a new, less explored challenge because of the need to direct glucose into the product pathway rather than the competing path to catabolize the sugar for biomass and energy production. Initial methods, such as deletion of glycolysis and other competing pathways, result in poor glucose uptake because of the cell's complex regulatory circuits. This project proposes to develop strains of E. coli that can efficiently take up glucose while also directing it to the glucaric acid pathway.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

这个小型企业技术转让(STTR)第二阶段项目的更广泛影响是以低成本、高纯度的生物为基础生产葡萄糖酸，这是一种应用广泛的化合物。这种葡萄糖酸的生产将使日常材料的石油来源，如衣服中的尼龙或两升瓶中的聚酯，广泛地转变为可再生资源产生的生物基产品。同样，这项技术将允许从水处理系统中使用的传统磷酸盐演变为更安全、更具成本效益的替代产品。拟议的项目将开发一种菌株、发酵工艺和可扩展的下游分离工作流程，以葡萄糖为原料生产低成本、高纯度的葡萄糖酸。微生物发酵是从纤维素糖等可再生资源中生产燃料和有价值的化学品的一个有吸引力的选择。微生物非常适合于碳水化合物原料的转化；已经证明，它们的代谢工程的几个例子可以将这些原料导向非天然化学品和具有工业价值的材料，通常作为石油产品的替代产品。另一方面，糖氧化产物构成了一个新的、较少被探索的挑战，因为需要将葡萄糖引导到产物途径中，而不是竞争的途径来分解糖以用于生物质和能源生产。最初的方法，如删除糖酵解和其他相互竞争的途径，会因为细胞复杂的调节电路而导致葡萄糖摄取不足。该项目建议开发能够有效地吸收葡萄糖的大肠杆菌菌株，同时将其引导到葡萄糖酸途径。这一奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。