I-Corps: Artificial Tissue Scaffolds and Cell Cultures Interlaced with Vasculature-like Micro-Channels

I-Corps：人造组织支架和细胞培养物与类似脉管系统的微通道交错

• 批准号: 1953890
• 负责人: Roman Voronov
• 金额: $ 5万
• 依托单位: New Jersey Institute of Technology
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-02-01 至 2022-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1953890&HistoricalAwards=false
• 关键词: Corps; Artificial; Tissue; Scaffolds; Cell

The broader impact/commercial potential of this I-Corps project is a novel engineered tissue culturing platform that will accelerate the translation of regenerative medicine technologies to the clinical market. According to the U.S. Dept. of Health & Human Services, only 10% of the 115k people in the U.S needed a lifesaving organ transplant in 2018 received it. A major obstacle to producing viable artificial tissues is product variability. The proposed technology overcomes that limitation by enabling non-disruptive control over the cell behavior at any location within the living artificial tissues as they are being cultured. Additionally, it allows for nondestructive analysis of the cell behavior, which will impact the markets where the cultures are used for things like production of biological molecules, toxicity and pharmaceutical testing, etc., by lowering experiment costs relative to the conventional (typically sacrificial) analysis. Lastly, the fact that the culturing platform is automated solves another major logistical hurdle to the adoption of such technologies, by allowing companies to provide computer codes to hospital staff instead of having to teach them custom culturing protocols for each new product. This I-Corps project will allow for better understanding of how the proposed cell culturing technology can offer solutions to the clinical market; while also providing technological insight to members of the tissue engineering industry, and opportunities for significant improvements to their existing culturing systems and practices. The proposed technology is a tissue engineering scaffold interlaced with vascular-like microchannels, which help to overcome major bottlenecks limiting the conventional biomanufacturing approaches. Additionally, it offers multiple benefits to other market segments that involve cell culturing. For example, the technology has been demonstrated to enable new discoveries by allowing researchers to perform localized collections of mini cell biopsies and effluent for ex-situ analysis; additive and subtractive cell operations in living cell cultures, tissue patterning and overgrowth removal; establishing dynamic drug gradients and custom delivery protocols; overcoming size-limitations by nourishing cells deep within large cultures and removing metabolic waste from those regions.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

这个I-Corps项目的更广泛的影响/商业潜力是一个新的工程组织培养平台，将加速再生医学技术向临床市场的转化。据美国国防部。根据卫生人类服务部的数据，2018年，美国11.5万人中只有10%的人需要进行挽救生命的器官移植。生产可行的人工组织的一个主要障碍是产品的可变性。 所提出的技术克服了这一限制，使非破坏性控制的细胞行为在任何位置的活人工组织，因为他们正在培养。 此外，它允许对细胞行为进行非破坏性分析，这将影响培养物用于生物分子生产、毒性和药物测试等的市场，通过相对于常规（通常为牺牲的）分析降低实验成本。 最后，培养平台是自动化的，这一事实解决了采用此类技术的另一个主要物流障碍，允许公司向医院工作人员提供计算机代码，而不必为每个新产品教他们定制培养方案。 这个I-Corps项目将使人们更好地了解拟议的细胞培养技术如何为临床市场提供解决方案;同时也为组织工程行业的成员提供技术见解，并为他们现有的培养系统和实践提供重大改进的机会。 所提出的技术是一种与血管样微通道交织的组织工程支架，这有助于克服限制传统生物制造方法的主要瓶颈。 此外，它还为涉及细胞培养的其他细分市场提供了多种好处。 例如，该技术已被证明能够促成新的发现，使研究人员能够进行微型细胞活检和流出物的局部收集，用于非原位分析;活细胞培养中的加性和减性细胞操作、组织图案化和过度生长去除;建立动态药物梯度和定制交付协议;克服大小-该奖项反映了NSF的法定使命，并被认为值得通过使用基金会的学术价值和更广泛的影响审查标准。

项目成果

Ranking migration cue contributions to guiding individual fibroblasts faced with a directional decision in simple microfluidic bifurcations

对迁移线索的贡献进行排名，以指导单个成纤维细胞在简单的微流体分叉中面临方向性决策

• DOI: 10.1093/intbio/zyz018
• 发表时间: 2019
• 期刊: Integrative Biology
• 影响因子: 2.5
• 作者: Pham, Quang Long;Tong, Anh;Rodrigues, Lydia N;Zhao, Yang;Surblyte, Migle;Ramos, Diomar;Brito, John;Rahematpura, Adwik;Voronov, Roman S
• 通讯作者: Voronov, Roman S

Automated Addressable Microfluidic Device for Minimally Disruptive Manipulation of Cells and Fluids within Living Cultures

• DOI: 10.1021/acsbiomaterials.9b01969
• 发表时间: 2020-03-01
• 期刊: ACS BIOMATERIALS SCIENCE & ENGINEERING
• 影响因子: 5.8
• 作者: Anh Tong;Quang Long Pham;Voronov, Roman
• 通讯作者: Voronov, Roman