RUI: Liposome Bilayer-Embedded Hydrophobic Palladium Nanoparticles for Selective Catalytic Reactions in Water

RUI：脂质体双层嵌入的疏水性钯纳米粒子用于水中的选择性催化反应

• 批准号: 1954659
• 负责人: Young-Seok Shon
• 金额: $ 21.32万
• 依托单位: California State University-Long Beach Foundation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-07-01 至 2024-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1954659&HistoricalAwards=false
• 关键词: RUI; Liposome; Bilayer; Embedded; Hydrophobic

Molecules that mimic the function of enzymes have been of interest for many years. Enzyme mimics provide advantages over natural enzymes as they are easily modified structures, improve stability, and lower cost. Mimicking the chemical reactivity of natural enzymes requires models that maintain the complex structures at and near the enzyme active site as these sites are responsible for that reactivity. In this project, Dr. Shon of California State University Long Beach is investigating a new approach to enzyme mimics where palladium nanoparticles are embedded in the lipid layer of a liposome, which resembles a cell membrane. The nanoparticle surface reactivity is modified by the attachment of various molecules to it. The resulting constructs may provide the level of control needed to simulate the interactions at an enzyme active site. Dr. Shon is actively engaged in educational programs and outreach activities that build upon his research to promote engagement of students ranging from high school students to master-level graduate students in science, technology, engineering and mathematics (STEM) disciplines. These activities include the Keck Energy and Materials Program (KEMP) for undergraduates and summer materials research internships in Dr. Shon’s laboratory for high school students. With funding from the Chemical Catalysis Program of the Division of Chemistry, Dr. Shon of California State University Long Beach (CSULB) is developing a fundamental understanding of how nanoparticle liposome embedding, lipid phase transition, membrane fluidity, and surface ligand density and composition of nanoparticles influence the non-covalent interactions between substrates and nanoparticles and determine the catalytic properties of the liposome bilayer-embedded palladium nanoparticles. For the investigation of the influence of liposome encapsulation, various lipids are used to study the effects of different phase transition temperatures. The molar ratio of lipids to palladium (Pd) nanoparticles are varied to observe the influence of membrane fluidity. Catalytic Pd nanoparticles with controlled core size and varying capping ligand density and structure are used for isolating the effect of surface ligands from other factors such as nanoparticle size, shape, and morphology and liposome structure. Understanding the effects of the surface composition and distribution of binary thiolate ligands adsorbed on Pd nanoparticle catalyst surfaces may distinguish and control the electronic and geometric contributions by the capping ligands. Ultimately, the findings are applied to the development of optimized lipid-nanoparticle hybrid therapeutic agents based on pro-drug activation and bioorthogonal enzymatic reaction. Dr. Shon is actively engaged in STEM education by exposing undergraduate and master's-level graduate students to the creative scientific research investigations. He actively recruits women and minority students, traditionally underrepresented groups in the STEM fields, to enhance the balanced advancement of research and education. He is also expanding a high school student research internship program in support of the broader impacts of the project.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

模仿酶功能的分子已经引起了很多年的关注。酶模拟物具有与天然酶相比的优势，因为它们很容易修改结构，提高稳定性和较低的成本。模仿天然酶的化学反应性需要模型，以维持酶活性位点和附近的复杂结构，因为这些位点是导致这种反应性的原因。在这个项目中，加利福尼亚州立大学长滩的Shon博士正在研究一种新方法，以模仿钯纳米颗粒嵌入脂质体的脂质层中，该脂质体类似于细胞膜。纳米颗粒表面反应通过各种分子的附着来改变。所得的构造可能会提供模拟酶活性位点相互作用所需的控制水平。 Shon博士积极从事教育计划和外展活动，这些活动以他的研究为基础，以促进从高中生到科学，技术，工程和数学（STEM）学科的硕士学位研究生的参与。这些活动包括Keck Energy and Materials计划（KEMP），针对Shon博士的高中生实验室的本科生和夏季材料研究实习。借助化学催化计划的资金，加利福尼亚州立大学长滩（CSULB）的肖博士正在对纳米颗粒脂质体嵌入，脂质相位过渡，膜流动性，膜流动性，表面配体和纳米颗粒之间的表面配体和纳米构成的属性构成分子和nanoparties之间的表面关系和组成的基本了解，并确定nanopartials and ananoptripe and cation and ananoptripes the ananoptripe the ananoptripe and aroptripation and aroptripation and arttriptripe and aroptripation and a nanoptripes双层包裹的钯纳米颗粒。为了投资脂质体封装的影响，使用各种脂质来研究不同相过渡温度的影响。脂质与钯（PD）纳米颗粒的摩尔比有所不同，以观察膜流动的影响。具有控制核心大小的催化PD纳米颗粒和不同的封盖配体密度和结构用于将表面配体的效果与其他因素（例如纳米颗粒大小，形状，形态和形态和脂质体结构）隔离。了解吸附在PD纳米颗粒催化剂表面上的表面组成和分布的作用，可以通过封盖配体来区分和控制电子和几何贡献。最终，这些发现应用于基于促毒物激活和生物正交酶促反应的优化脂质纳米粒子杂化剂的开发。 Shon博士通过将本科和硕士级别的研究生暴露于创造性的科学研究投资中，从而积极从事STEM教育。他积极招募妇女和少数族裔学生，传统上代表人数不足，以增强研究和教育的平衡进步。他还正在扩大一项高中学生研究实习计划，以支持该项目的广泛影响。该奖项反映了NSF的法定任务，并使用基金会的知识分子优点和更广泛的影响评估标准，认为通过评估被认为是宝贵的支持。