Catalyst Project: Combating ESKAPE pathogens through discovery of novel antimicrobial and antibiofilm molecules

催化剂项目：通过发现新型抗菌剂和抗生物膜分子来对抗 ESKAPE 病原体

• 批准号: 2000260
• 负责人: Danielle Graham
• 金额: $ 17.92万
• 依托单位: Fayetteville State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-15 至 2024-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2000260&HistoricalAwards=false
• 关键词: Catalyst; Project; Combating; ESKAPE; pathogens

Catalyst Projects provide support for Historically Black Colleges and Universities to work towards establishing research capacity of faculty to strengthen science, technology, engineering and mathematics undergraduate education and research. It is expected that the award will further the faculty member's research capability, improve research and teaching at the institution, and involve undergraduate students in research experiences. This project at Fayetteville State University intends to look at soil microbes and identify the chemicals they produce to kill bacteria. The identification of these microbes and chemicals will have implications in understanding biological films. Fayetteville State University undergraduate students will have the opportunity to perform research.The goal of the proposed study is to collect soil from various environmental areas such as oceanic, mountainous, and natural preserve regions, define the physiological cultural conditions and identify the chemistry of small molecules bacteria produce in efforts to understand how these organisms kill other bacteria, particularly ESKAPE pathogens, while not harming themselves. The specific objectives of the proposed research are 1) to identify and characterize the physical and chemical properties of antimicrobial producing soil microbes that inhibit safe-ESKAPE relative strains, and 2) determine the effect of soil microbes on biofilm formation, dispersal and gene expression of safe ESKAPE relative strains. The findings from the research outlined in this proposal will enhance our understanding of microbe-microbe interactions and further advance the knowledge of antibiotic discovery using a tried and true method of mining soil microbes coupled with a metagenomic and computational approach. The implementation of the proposed research will provide an opportunity for underrepresented groups to gain hands-on research experience, access state of the art equipment as well as cutting edge techniques through the collaboration with UNC Chapel Hill and develop a collaborative network of student researchers and faculty.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

催化剂项目为历史悠久的黑人学院和大学提供支持，努力建立教师的研究能力，以加强科学、技术、工程和数学本科教育和研究。预计该奖项将进一步提高教师的研究能力，改善机构的研究和教学，并让本科生参与研究经验。费耶特维尔州立大学的这个项目旨在研究土壤微生物并识别它们产生的杀死细菌的化学物质。这些微生物和化学物质的识别将对理解生物膜产生影响。费耶特维尔州立大学的本科生将有机会进行研究。拟议研究的目标是从海洋、山区和自然保护区等不同环境区域收集土壤，确定生理培养条件并鉴定细菌产生的小分子化学成分，以了解这些生物体如何杀死其他细菌，特别是 ESKAPE 病原体，同时不伤害自身。本研究的具体目标是 1) 鉴定和表征抑制安全 ESKAPE 相关菌株的产抗菌土壤微生物的物理和化学特性，2) 确定土壤微生物对安全 ESKAPE 相关菌株生物膜形成、扩散和基因表达的影响。该提案中概述的研究结果将增强我们对微生物与微生物相互作用的理解，并利用经过验证的土壤微生物挖掘方法以及宏基因组和计算方法，进一步推进抗生素发现的知识。拟议研究的实施将为代表性不足的群体提供机会，通过与北卡罗来纳大学教堂山分校的合作，获得实践研究经验、获得最先进的设备以及尖端技术，并发展学生研究人员和教师的协作网络。该奖项反映了 NSF 的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。