Collaborative Research: Network Cluster: Geomicrobiology and Biogeochemistry in the Critical Zone

合作研究：网络集群：关键区域的地球微生物学和生物地球化学

• 批准号: 2012403
• 负责人: William McDowell
• 金额: $ 23.65万
• 依托单位: University of New Hampshire
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-01 至 2022-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2012403&HistoricalAwards=false
• 关键词: Collaborative; Research; Network; Cluster; Geomicrobiology

The critical zone is the active upper crust of the Earth, from the lowest groundwater to the top of the highest tree. Critical Zone science is at an important juncture: it is incorporating life in more quantitative ways. This project will evaluate the contribution of the microorganisms that drive so many of the dynamics of the Critical Zone, addressing crucial gaps in our knowledge of surface and deep soil microbes and their functions. The major goal of this research is to develop a predictive understanding of the role of microbes as drivers of Critical Zone biogeochemistry and soil formation. The researchers’ preliminary investigations across the former Critical Zone Observatory Network identified variable patterns in microbial biodiversity with increasing soil depth across sites, with some sites showing little to no change with depth, and others a complete turnover of the microbial community members. The current project will illuminate the mechanisms underlying these trends. This project will train numerous undergraduate students, most of whom will be underrepresented minorities at several Hispanic Serving Institutions (HSIs) and high schools in underserved communities across California. Results will be disseminated to the greater public through a series of webinars that will provide real life examples of how to address Critical Zone science questions by leveraging diverse scientific backgrounds, as well as through incorporation into the “Welcome to the Critical Zone” public museum exhibit at The University of Arizona’s Flandrau Science Center.This project will address the hypothesis that soil type, hydrology, and lithology are key determinants of the depth to which surface influences such as vegetation and climate drive microbial community composition, diversity, and activity. Specifically, where soils are deep or are derived from nutrient- rich bedrock, the researchers hypothesize that rooting depths will be greater, resulting in a greater similarity between surface and deep soil microbial communities compared to those from shallower soils or those developed from less nutrient-rich substrates. In this proposed project, the researchers will use a novel combination of DNA and RNA sequencing, real-time soil gas and environmental monitoring, quantified enzyme activities, detailed soil organic matter and isotopic analyses, as well as root abundances to address the hypothesis. To amplify the impact of this approach, the project will develop a unique and detailed geomicrobiology characterization of key indicator species of bacteria, archaea and fungi, grouped to distinguish those that are surface-based and those representative of bedrock interactions. This novel framework will be tested across years and will generate a resource for the larger NSF Geoscience community for years to come. This project will also form an important interdisciplinary aspect of the new Critical Zone Collaborative Network, through collaborations with various other Clusters and the Hub. This award is co-funded by the Critical Zone Collaborative Network of the Division of Earth Sciences and the Ecosystem Sciences cluster of the Division of Environmental Biology.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

关键区域是地球活跃的上地壳，从最低的地下水到最高的树顶。关键区域科学正处于一个重要关头：它正在以更定量的方式融入生命。该项目将评估驱动关键区如此多动态的微生物的贡献，解决我们对表层和深层土壤微生物及其功能的认识中的关键差距。这项研究的主要目标是对微生物作为关键区生物地球化学和土壤形成驱动因素的作用进行预测性理解。研究人员对前关键区域观测网络进行的初步调查发现，随着不同地点土壤深度的增加，微生物生物多样性的变化模式，一些地点几乎没有随深度变化，而另一些地点则显示出微生物群落成员的完全更替。当前的项目将阐明这些趋势背后的机制。该项目将培训大量本科生，其中大多数是加利福尼亚州服务欠缺社区的几所西班牙裔服务机构 (HSI) 和高中中代表性不足的少数族裔。研究结果将通过一系列网络研讨会向广大公众传播，这些研讨会将提供如何利用不同科学背景以及纳入亚利桑那大学弗兰德劳科学中心的“欢迎来到关键区”公共博物馆展览来解决关键区科学问题的现实例子。该项目将解决这样的假设：土壤类型、水文和岩性是地表影响深度的关键决定因素，例如植被和土壤。 气候驱动微生物群落的组成、多样性和活动。具体来说，在土壤较深或源自营养丰富的基岩的地方，研究人员假设，与较浅的土壤或营养较不丰富的基质发育的土壤相比，根系深度会更大，导致表层和深层土壤微生物群落之间的相似性更大。在这个拟议的项目中，研究人员将使用 DNA 和 RNA 测序、实时土壤气体和环境监测、量化酶活性、详细土壤有机质和同位素分析以及根丰度的新颖组合来解决这一假设。为了扩大这种方法的影响，该项目将对细菌、古细菌和真菌的关键指示物种进行独特而详细的地球微生物学表征，并将其分组以区分基于表面的物种和代表基岩相互作用的物种。这个新颖的框架将经过数年的测试，并将在未来几年为更大的 NSF 地球科学界提供资源。通过与其他各个集群和中心的合作，该项目还将形成新的关键区域协作网络的重要跨学科方面。该奖项由地球科学部关键区域合作网络和环境生物学部生态系统科学集群共同资助。该奖项反映了 NSF 的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Hydrological Mapping in the Luquillo Experimental Forest: New Local Datum Improves Watershed Ecological Knowledge

卢基约实验森林的水文测绘：新的当地数据提高了流域生态知识

• DOI: 10.3390/hydrology8010054
• 发表时间: 2021
• 期刊: Hydrology
• 影响因子: 3.2
• 作者: Leon, Miguel C.;Heartsill-Scalley, Tamara;Santiago, Iván;McDowell, William H.
• 通讯作者: McDowell, William H.

Landslides, hurricanes, and sediment sourcing impact basin-scale erosion estimates in Luquillo, Puerto Rico

• DOI: 10.1016/j.epsl.2021.116821
• 发表时间: 2021-03-08
• 期刊: EARTH AND PLANETARY SCIENCE LETTERS
• 影响因子: 5.3
• 作者: Grande, Alexandra;Schmidt, Amanda H.;Caffee, Marc W.
• 通讯作者: Caffee, Marc W.