Central facility for mass spectrometry and proteomics

中央项目Z4N将用最先进的质谱技术支持CRU 216的所有项目。我们将应用定量质谱法来鉴定特定的蛋白质相互作用伙伴（例如，Ral蛋白），用于鉴定小分子药物的细胞靶标（例如，二苯二酚类药物），并用于分析翻译调节背景下新合成的蛋白质。我们将建立一个先进的工作流程，用于磷酸蛋白质组学和选择性富集激酶应用头部偶联广泛选择性激酶抑制剂。我们将应用磷酸蛋白组分析，例如，用于识别激酶NIK和IKK1的直接和间接靶标，以及阐明Raf激酶的mek独立信号转导。

The central project Z4N will support all projects of the CRU 216 with state-of-the-art mass spectrometry. We will apply quantitative mass spectrometry for the identification of specific protein interaction partners (for example, of Ral proteins), for the identification of cellular targets of small molecule drugs (for example, of dioncoquinones), and for the analysis of newly synthesized proteins in the context of translational regulation. We will set up an advanced workflow for phosphoproteomics and for the selective enrichment of kinases applying a bead-coupled broadly selective kinase inhibitor. We will apply phosphoproteome analyses, for example, for the identification of direct and indirect targets of the kinases NIK and IKK1, and for elucidating MEK-independent signal transduction of Raf kinases.