RUI: State-dependent modulation of visuomotor reflexes across Drosophila species

RUI：果蝇物种视觉运动反射的状态依赖性调节

• 批准号: 2016188
• 负责人: Sara Wasserman
• 金额: $ 63.81万
• 依托单位: Wellesley College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-01 至 2025-07-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2016188&HistoricalAwards=false
• 关键词: RUI; State; dependent; modulation; visuomotor

From the moment we wake up in the morning until the moment we fall asleep we are constantly barraged with sensory stimuli. The buzzing of the alarm, the dinging of email alerts, yet somehow, we identify the salient sensory stimuli and ignore the rest in order to generate an appropriate behavioral response each moment. Which stimuli we identify to be salient could depend on additional factors such as our external and internal environments. The overall goal of this project is to explore how the brain integrates our internal and external environment to modify the perception of visual cues to promote survival in different habitats. Specifically, this proposal will examine the contributions of anatomical and neural circuit changes in guiding visually evoked behaviors in Drosophila species living in distinct ecological habitats. The proposed work will support the training of undergraduates at Wellesley College, an all-women’s institution. Students will be actively involved in all aspects of experimental design, data collection, analysis, and manuscript preparation. Wellesley’s student body is geographically, culturally, and economically diverse and all efforts will be made to involve members of underrepresented groups. Experiments outlined in this proposal will be carried out by undergraduate research students both in the lab as well as integrated into an upper-level elective course. Findings from this work will be shared at scientific meetings as well as with high school students and elementary and secondary school teachers. How can an organism survive in ever-changing environmental conditions? They must be able to generate adaptive behavior by applying flexibility as they: (1) discriminate salient sensory signals from background, (2) assign value – attractive or aversive – to such stimuli and (3) integrate these stimuli with the current environmental context and internal physiological state. While inroads have been made in understanding the processes of discrimination and assigning value, much remains unknown about where and how short-term (hours timescale) changes in internal state are integrated to drive contextually appropriate behavior. Work across organisms has investigated how internal state modulates perception of odor, pheromone, temperature, and water, however, little work has examined how internal state differentially modulates the perception of visual stimuli of organisms from environments with distinct visual ecologies (forest and desert) that may have imposed very different evolutionary pressures. The goal of this proposal is to examine the contributions of anatomical and neural circuit changes in guiding visually evoked behaviors in Drosophila species living in distinct ecological habitats. These experiments will elucidate the impact of ecological habitats on the generation of state-dependent behavior and neuromodulatory circuits in order to understand how behavioral responses and the neural circuits involved may have been altered by differential evolutionary pressures. The project will utilize a multifaceted approach: (1) genetic manipulation to allow control over specific neural pathways, (2) visual and olfactory ‘virtual-reality’ flight simulators to measure sensory motor integration that drives adaptive behavior, and (3) in-vivo two photon calcium imaging as an indicator of neural activity.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

从我们早上醒来的那一刻到我们入睡的那一刻，我们不断地被感官刺激所包围。闹钟的嗡嗡声，电子邮件的警报声，但不知何故，我们识别出了突出的感觉刺激，而忽略了其余的，以便每时每刻都能产生适当的行为反应。我们认为哪些刺激是显著的，可能取决于其他因素，如我们的外部和内部环境。这个项目的总体目标是探索大脑如何整合我们的内部和外部环境，以调整对视觉线索的感知，以促进在不同栖息地的生存。具体地说，这项建议将研究解剖和神经回路变化在指导生活在不同生态栖息地的果蝇物种的视觉诱发行为方面的贡献。拟议的工作将支持韦尔斯利学院的本科生培训，这是一家全女性机构。学生将积极参与实验设计、数据收集、分析和手稿准备的方方面面。卫尔斯理的学生群体在地理、文化和经济上都是多样化的，将尽一切努力让代表性不足的群体成员参与进来。这份提案中概述的实验将由本科生在实验室进行，也将整合到高级选修课中。这项工作的发现将在科学会议上以及与高中生和中小学教师分享。生物体如何在不断变化的环境条件下生存？他们必须能够通过应用灵活性来产生适应行为，因为他们：(1)区分突出的感觉信号和背景；(2)为这些刺激分配价值--吸引或厌恶--以及(3)将这些刺激与当前环境背景和内部生理状态相结合。虽然在理解歧视和分配价值的过程方面已经取得了进展，但关于在哪里以及如何整合内部状态的短期(时间尺度)变化来驱动与背景相适应的行为，仍有许多未知之处。跨生物体的工作已经研究了内部状态如何调节对气味、信息素、温度和水的感知，然而，很少有工作研究内部状态如何差异化地调节来自不同视觉生态环境(森林和沙漠)的生物体对视觉刺激的感知，这些环境可能施加了非常不同的进化压力。这项建议的目的是研究解剖和神经回路变化在指导生活在不同生态环境中的果蝇物种的视觉诱发行为方面的贡献。这些实验将阐明生态栖息地对状态依赖行为和神经调节回路产生的影响，以了解不同进化压力如何改变行为反应和涉及的神经回路。该项目将利用多方面的方法：(1)基因操作，以允许控制特定的神经通路，(2)视觉和嗅觉‘虚拟现实’飞行模拟器，以测量驱动适应行为的感觉运动整合，以及(3)体内双光子钙成像作为神经活动的指示器。该奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Internal State: Dynamic, Interconnected Communication Loops Distributed Across Body, Brain, and Time.

• DOI: 10.1093/icb/icab101
• 发表时间: 2021-10-04
• 期刊: Integrative and comparative biology
• 影响因子: 2.6
• 作者: Kanwal JK;Coddington E;Frazer R;Limbania D;Turner G;Davila KJ;Givens MA;Williams V;Datta SR;Wasserman S
• 通讯作者: Wasserman S