PFI-TT: Accelerated Detection of Successful Crop Production

PFI-TT：加速检测成功的作物生产

• 批准号: 2016545
• 负责人: R Keith Slotkin
• 金额: $ 24.82万
• 依托单位: Donald Danforth Plant Science Center
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-15 至 2023-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2016545&HistoricalAwards=false
• 关键词: PFI; TT; Accelerated; Detection; Successful

The broader impact/commercial potential of this Partnerships for Innovation - Technology Translation (PFI-TT) project is the acceleration of modern crop production by removing a key roadblock. Currently, the time and monetary investment required to generate a new crop line produces accessibility inequities to the latest improved strains. Through this project, a technology will be developed to reduce the time and cost to the commercial production pipeline. This technology will help understand the earliest steps of the crop production pipeline on the molecular level, so that projects and lines that are destined to fail can be quickly identified and removed from the production line. This early quality control will reduce the time and investment associated with the crop production pipeline, reducing the commercial investment in the process and improving accessibility to potential new crops. The proposed project aims to detect off-type or “aberrant” RNAs in new crop lines in the early event sorting phase, after the process of transgenesis. These aberrant RNAs are unwanted, as they fail the cell’s RNA quality control mechanism and inhibit future transcription from these regions. The overall scientific goals of this project are to develop a technology that detects all the RNA generated by a plant transgene or any locus of interest, and then use this predictive information to inform breeders which lines should progress in the crop production pipeline. The research objectives are to refine the technology to identify all species of RNA generated by a transgene, and then test this predive potential on real-world crop lines provided by an industry partner.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

这一创新-技术转化伙伴关系(PFI-TT)项目的更广泛影响/商业潜力是通过消除一个关键障碍来加速现代作物生产。目前，产生一种新的作物品系所需的时间和金钱投资造成了对最新改良品系的可及性不平等。通过该项目，将开发一种技术，以减少商业生产流水线的时间和成本。这项技术将有助于从分子层面了解作物生产流水线的最早步骤，以便快速识别注定失败的项目和生产线，并将其从生产线上移除。这种早期的质量控制将减少与作物生产管道相关的时间和投资，减少这一过程中的商业投资，并改善潜在新作物的可及性。拟议的项目旨在在转基因过程之后的早期事件分类阶段检测新作物品系中的非类型或“异常”RNA。这些异常的RNA是不受欢迎的，因为它们使细胞的RNA质量控制机制失效，并抑制这些区域未来的转录。该项目的总体科学目标是开发一种技术，该技术可以检测植物转基因或任何感兴趣的位点产生的所有RNA，然后使用这种预测性信息来通知育种者哪些品系应该在作物生产管道中取得进展。研究目标是改进技术，以识别由转基因产生的所有物种的RNA，然后在由行业合作伙伴提供的现实世界作物线上测试这种预测潜力。这一奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。