MRI: Acquisition of a laser scanning confocal microscope for cellular imaging for faculty and students at Baruch College

MRI：为巴鲁克学院的教职员工和学生购买用于细胞成像的激光扫描共焦显微镜

• 批准号: 2019021
• 负责人: Krista Dobi
• 金额: $ 44.69万
• 依托单位: CUNY Baruch College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-01 至 2023-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2019021&HistoricalAwards=false
• 关键词: MRI; Acquisition; laser; scanning; confocal

An award is made to Baruch College, City University of New York to provide support for the acquisition of an advanced confocal microscopy system with super-resolution capacity. The new system will support the current and foreseeable critical research needs of the faculty in the Department of Natural Sciences, which include multi-color imaging of fixed and live samples, time-lapse imaging, quantitative fluorescence, photo-activation, visualization of subcellular structures and organelles, spectral analysis of bioluminescent organisms, 3-D rendering and imaging of complex tissues. The system's versatility makes it capable of accommodating a wide variety of experimental approaches now and in the future and will maximize the number of researchers at Baruch who can use the new equipment for years to come. In addition to faculty research, the confocal will also be used to train graduate and undergraduate students in modern research practices and technologies as part of individually mentored research and classroom instruction. The new confocal imaging system will be used by at least nine research groups within the Department of Natural Sciences and their collaborators, whose broad and multidisciplinary research objectives include the determination of hormone signaling mechanisms, elucidation of the role of reactive oxygen species in the nervous system, identification of bioluminescence in marine organisms, investigation of melanin in fungal pathogenesis, determination of muscle attachment site selection, elucidation of gene regulatory pathways governing vertebrate differentiation, identification of environmental impacts on insect behavior, and classification of morphological characteristics in speciation. These data will be publicized in the form of peer-reviewed publications and presentations at scientific meetings, as well as directly shared with interested researchers and collaborators.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

纽约市立大学巴鲁克学院获得一项奖项，为购买具有超分辨率能力的先进共焦显微镜系统提供支持。 新系统将支持自然科学系教师当前和可预见的关键研究需求，其中包括固定和活体样本的多色成像、延时成像、定量荧光、光激活、亚细胞结构和细胞器的可视化、生物发光生物体的光谱分析、复杂组织的3D渲染和成像。该系统的多功能性使其能够适应现在和未来的各种实验方法，并将最大限度地增加巴鲁克研究人员的数量，让他们在未来几年可以使用新设备。除了教师研究之外，共聚焦还将用于培训研究生和本科生现代研究实践和技术，作为单独指导的研究和课堂教学的一部分。新的共聚焦成像系统将由自然科学系内的至少九个研究小组及其合作者使用，其广泛和多学科的研究目标包括确定激素信号传导机制、阐明活性氧在神经系统中的作用、识别海洋生物中的生物发光、研究真菌发病机制中的黑色素、确定肌肉附着位点选择、 阐明控制脊椎动物分化的基因调控途径，识别环境对昆虫行为的影响，以及物种形成中形态特征的分类。这些数据将以同行评审出版物和科学会议上演示的形式公布，并直接与感兴趣的研究人员和合作者共享。该奖项反映了 NSF 的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Mitochondrial ROS signaling during synaptic potentiation

突触增强过程中线粒体 ROS 信号传导

• DOI: 10.1016/j.bpj.2021.11.223
• 发表时间: 2022
• 期刊: Biophysical journal
• 影响因子: 3.4
• 作者: Stavrovskaya, Irina;Mitaishvilli, Erna;Morin, Bethany Kristi;Walls, Aria;Terry, Grace;Peixoto, Pablo M.
• 通讯作者: Peixoto, Pablo M.