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Viscoelastic Characterization of Multicellular Tissues in Physiologically Relevant Conditions through Probe Indentation

通过探针压痕对生理相关条件下的多细胞组织进行粘弹性表征

基本信息

批准号：
2019507
负责人：
Santiago Solares
金额：
$ 49.25万
依托单位：
George Washington University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2020
资助国家：
美国
起止时间：
2020-08-01 至 2024-05-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=2019507&HistoricalAwards=false
关键词：
Viscoelastic Characterization Multicellular Tissues Physiologically

项目摘要

This award will produce new knowledge about the mechanical behavior of cancer tissues. Specifically, microscopic measurements of mechanical behavior will be made as the disease progresses. In this project, the focus will be on skin cancers. However, the findings will be applicable to other diseases for which tissues undergo mechanical changes such as arteriosclerosis. The project will deliver the new methods and sensors capable of use in the native liquid environment of biological materials. Establishing a link between cancer progression and mechanical changes in tissues could ultimately have applications to human health. For example, the results of this work could enable the development of patient-specific disease databases, and standards for mechanical tissue behaviors. These might eventually allow classification by individual patient history and information. This could have a transformative effect on medicine and biology research, as mechanical information is not yet broadly considered, much less classified in engineering terms. This research involves several disciplines, including medicine, biology, materials science and mechanical engineering. The multi-disciplinary approach will help broaden participation of underrepresented groups in research and positively impact engineering education. Biological materials are viscoelastic and may respond according to multiple characteristic timescales which are generally not captured in existing mechanical characterization methods, especially micro- and nanoscale approaches. Such methods often reduce the mechanical response to elastic quantities, such as the Young’s modulus, or combinations of a few quantities that empirically combine dissipative and elastic behaviors. This project will develop viscoelastic property inversion methods, whereby the sample is probed using a microscale indenter, and the measured response is translated into a generalized viscoelastic model containing an arbitrary number of characteristic times. This approach will allow quantification of material behaviors in engineering units, which will allow comparison of different specimens measured on different instruments by different researchers. The project also includes development of the indentation apparatus, which will resemble an atomic force microscope, but will be tailored to the typical relaxation timescales observed in biological materials, which are significantly larger than those probed by commercial atomic force microscopes. The project will involve computational and analytical modeling of the fluid dynamics surrounding the probe and sample to incorporate corrections due to fluid forces, which can in some cases obscure the probe-sample interactions for soft materials. Finally, the new apparatus and methods will be employed to acquire the viscoelastic signature of melanoma cancer and healthy skin cell lines.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该奖项将产生关于癌症组织力学行为的新知识。具体而言，随着疾病的进展，将进行机械行为的显微镜测量。在这个项目中，重点将是皮肤癌。然而，这些发现将适用于其他组织发生机械变化的疾病，如动脉硬化。该项目将提供能够在生物材料的原生液体环境中使用的新方法和传感器。在癌症进展和组织中的机械变化之间建立联系最终可能会应用于人类健康。例如，这项工作的结果可以开发患者特定的疾病数据库和机械组织行为的标准。这些最终可能允许通过个体患者的病史和信息进行分类。这可能对医学和生物学研究产生变革性影响，因为机械信息尚未得到广泛考虑，更不用说在工程术语中进行分类。这项研究涉及多个学科，包括医学，生物学，材料科学和机械工程。多学科方法将有助于扩大代表性不足的群体在研究中的参与，并对工程教育产生积极影响。生物材料是粘弹性的，并且可以根据多个特征时间尺度响应，这些特征时间尺度通常在现有的机械表征方法（特别是微米和纳米级方法）中不被捕获。这样的方法通常减少对弹性量的机械响应，例如杨氏模量，或经验上结合了耗散和弹性行为的联合收割机的几个量的组合。本项目将开发粘弹性性质反演方法，即使用微型压头探测样品，并将测量的响应转换为包含任意数量的特征时间的广义粘弹性模型。这种方法将允许在工程单位中量化材料行为，这将允许比较不同研究人员在不同仪器上测量的不同样品。该项目还包括压痕装置的开发，该装置类似于原子力显微镜，但将针对生物材料中观察到的典型弛豫时间尺度进行定制，这些时间尺度明显大于商业原子力显微镜探测的时间尺度。该项目将涉及探针和样品周围流体动力学的计算和分析建模，以纳入由于流体力引起的校正，这在某些情况下可能会掩盖软材料的探针-样品相互作用。最后，新的设备和方法将用于获取黑色素瘤癌症和健康皮肤细胞系的粘弹性特征。该奖项反映了NSF的法定使命，并已被认为是值得通过使用基金会的智力价值和更广泛的影响审查标准进行评估的支持。

项目成果

期刊论文数量（8）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Frequency-dependent nanomechanical profiling for medical diagnosis.

DOI：
10.3762/bjnano.13.122
发表时间：
2022
期刊：
Beilstein journal of nanotechnology
影响因子：
3.1
作者：
Solares SD;Cartagena-Rivera AX
通讯作者：
Cartagena-Rivera AX

Shear-induced migration of a viscous drop in a viscoelastic liquid near a wall at high viscosity ratio: Reverse migration

高粘度比的粘弹性液体中粘弹性液体中粘性液滴的剪切引起的迁移：反向迁移

DOI：
10.1016/j.jnnfm.2022.104751
发表时间：
2022
期刊：
Journal of Non-Newtonian Fluid Mechanics
影响因子：
3.1
作者：
Mukherjee, Swarnajay;Tarafder, Anik;Malipeddi, Abhilash Reddy;Sarkar, Kausik
通讯作者：
Sarkar, Kausik

Pair interactions between viscous drops in a viscoelastic matrix in free shear: Transition from passing to tumbling trajectories

自由剪切中粘弹性基质中粘性液滴之间的成对相互作用：从通过轨迹到翻滚轨迹的转变