RAPID: NMR Characterization of SARS-CoV-2 Proteins

RAPID：SARS-CoV-2 蛋白的 NMR 表征

• 批准号: 2030601
• 负责人: Jeffrey Hoch
• 金额: $ 20万
• 依托单位: University of Connecticut Health Center
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-05-01 至 2022-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2030601&HistoricalAwards=false
• 关键词: RAPID; NMR; Characterization; SARS; CoV

The award to the University of Connecticut supports research that will help in understanding the role of non-structural proteins in the SARS-COV-2 virus. The researchers will use advanced chemical analytic techniques to look at protein structure in solution to complement other on-going research that looks at either frozen or crystallized protein samples. Results from these studies will contribute vital information to our understanding of SARS-COV-2 biology and may help to inform on the selection and development of therapeutic treatments. Data from these studies will be rapidly disseminated through publicly available repositories as part of the broader impact plan so that other researchers can leverage the experimental outcomes in their own studies. The resources and data will also be used to support graduate student training activities. The study findings will be published in peer-reviewed journals and shared at scientific meetings.This project will use heterologous protein expression systems and nuclear magnetic resonance (NMR) techniques to investigate the role of non-structural proteins encoded by the SARS-COV-2 genome. Empirical assessment of dynamics and disorder in solution using NMR spectroscopy will be used to complement or validate predictions based on crystal structures, and yield insight into catalytic mechanism, allosteric regulation, and antigenicity. Chemical shift profiling studies will be performed to aid in the identification of metabolites that bind to SARS-CoV-2 proteins to advance functional annotation. Resources and data generated from this study will be deposited in the Biological Magnetic Resonance Data Bank (BMRB) or shared through other means as a public resource. This RAPID award is made by the Division of Biological Infrastructure (DBI) using funds from the Coronavirus Aid, Relief, and Economic Security (CARES) Act.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

授予康涅狄格大学的奖项支持有助于了解非结构蛋白在SARS-COV-2病毒中的作用的研究。研究人员将使用先进的化学分析技术来研究溶液中的蛋白质结构，以补充其他正在进行的研究，这些研究着眼于冷冻或结晶的蛋白质样本。这些研究的结果将有助于我们了解SARS-COV-2生物学，并可能有助于为治疗方法的选择和开发提供信息。这些研究的数据将作为更广泛影响计划的一部分，通过公开可用的储存库迅速传播，以便其他研究人员可以在自己的研究中利用实验结果。这些资源和数据还将用于支持研究生培训活动。这项研究结果将发表在同行评议的期刊上，并在科学会议上分享。该项目将使用异源蛋白质表达系统和核磁共振技术来研究SARS-COV-2基因组编码的非结构蛋白质的作用。使用核磁共振波谱对溶液中的动力学和无序进行经验评估将被用于补充或验证基于晶体结构的预测，并获得对催化机制、变构调节和抗原性的洞察。将进行化学位移谱研究，以帮助识别与SARS-CoV-2蛋白结合的代谢物，以推进功能注释。这项研究产生的资源和数据将存放在生物磁共振数据库(BMRB)中，或作为公共资源通过其他方式共享。这一快速奖项由生物基础设施部(DBI)利用冠状病毒援助、救济和经济安全(CARE)法案的资金做出。该奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Backbone and Ile, Leu, Val methyl group resonance assignment of CoV-Y domain of SARS-CoV-2 non-structural protein 3.

• DOI: 10.1007/s12104-021-10059-y
• 发表时间: 2022-04
• 期刊: Biomolecular NMR assignments
• 影响因子: 0.9
• 作者: Pustovalova Y;Gorbatyuk O;Li Y;Hao B;Hoch JC
• 通讯作者: Hoch JC