EAGER: Toward determining the key interactions between the SARS-CoV2 virus components and host cells

EAGER:致力于确定 SARS-CoV2 病毒成分与宿主细胞之间的关键相互作用

基本信息

  • 批准号:
    2031790
  • 负责人:
  • 金额:
    $ 30万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Standard Grant
  • 财政年份:
    2020
  • 资助国家:
    美国
  • 起止时间:
    2020-06-15 至 2023-05-31
  • 项目状态:
    已结题

项目摘要

This project will bring rigorous biophysical knowledge to select protein components of the SARS-CoV2 virus that has catastrophic effects on human health, activity, and economic well-being. Critical events in the viral infection cycle include interaction of the virus with susceptible host cells and a variety of cellular responses post-infection. Experimental studies will be performed to characterize proteins that participate in these stages. By combining the extensive thermodynamic and structural expertise of two research groups, key structural features and molecular interactions will be described and quantified. The data will provide a framework to test the findings in cell-based infectivity assays. Two graduate students will be trained, and the results of this project will be used as the basis for a seminar course on coronaviruses directed at biophysics undergraduate and graduate students to develop future workforce with experience in this area. A critical interaction between SARS-CoV-2 and host is that between the receptor binding domain (RBD) of the viral spike transmembrane protein and the S1 domain of the human ACE2 transmembrane protein. Binding is required for infection, determining the host range that a particular virus can infect. This project will quantify this critical interaction thermodynamically, determine the virus-host specificity profile, test various mechanisms for the origin and zoonotic events that led to the SARS-CoV-2 pandemic, and generate soluble mimics with favorable stabilities and specificities that can be used to limit or prevent infection. In addition, three accessory proteins, Orf3a, Orf7a, and Orf8, that interfere with or hijack host cellular processes will be characterized, both structurally and functionally using various spectroscopic and hydrodynamic methods. To further probe the evolutionary origins of coronaviruses and their host specificities, ancestral sequence reconstruction methods and consensus design will be applied to these targets.This EAGER award is made by the Molecular Biophysics Program in the Division of Molecular and Cellular Biosciences, using funds from the Coronavirus Aid, Relief, and Economic Security (CARES) Act.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
该项目将带来严格的生物物理知识,以选择对人类健康,活动和经济福祉具有灾难性影响的SARS-CoV 2病毒的蛋白质组分。 病毒感染周期中的关键事件包括病毒与易感宿主细胞的相互作用以及感染后的各种细胞反应。将进行实验研究,以表征参与这些阶段的蛋白质。通过结合两个研究小组广泛的热力学和结构专业知识,将描述和量化关键的结构特征和分子相互作用。这些数据将提供一个框架,以测试基于细胞的感染性试验中的发现。 两名研究生将接受培训,该项目的结果将用作针对生物物理本科生和研究生的冠状病毒研讨会课程的基础,以培养未来在该领域具有经验的劳动力。SARS-CoV-2与宿主之间的关键相互作用是病毒刺突跨膜蛋白的受体结合结构域(RBD)与人ACE 2跨膜蛋白的S1结构域之间的相互作用。结合是感染所必需的,决定了特定病毒可以感染的宿主范围。该项目将定量这种关键的相互作用,确定病毒-宿主特异性概况,测试导致SARS-CoV-2大流行的起源和人畜共患病事件的各种机制,并产生具有良好稳定性和特异性的可溶性模拟物,可用于限制或预防感染。此外,三个辅助蛋白,Orf 3a,Orf 7a和Orf 8,干扰或劫持宿主细胞过程的特点,结构和功能,使用各种光谱和流体动力学方法。为了进一步探索冠状病毒的进化起源及其宿主特异性,祖先序列重建方法和共识设计将应用于这些靶点。EAGER奖由分子和细胞生物科学部的分子生物物理学计划颁发,使用冠状病毒援助,救济,该奖项反映了NSF的法定使命,并通过使用基金会的知识价值和更广泛的影响审查标准进行评估,被认为值得支持。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Juliette Lecomte其他文献

A Histidine-Lysine Axial Ligand Switch in a Hemoglobin
  • DOI:
    10.1016/j.bpj.2017.11.2246
  • 发表时间:
    2018-02-02
  • 期刊:
  • 影响因子:
  • 作者:
    Dillon Nye;Matthew Preimesberger;Ananya Majumdar;Juliette Lecomte
  • 通讯作者:
    Juliette Lecomte

Juliette Lecomte的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Juliette Lecomte', 18)}}的其他基金

Building quaternary structure in heme proteins
构建血红素蛋白的四级结构
  • 批准号:
    2003950
  • 财政年份:
    2020
  • 资助金额:
    $ 30万
  • 项目类别:
    Standard Grant
Structural and dynamic characterization of 2/2 hemoglobins
2/2 血红蛋白的结构和动态表征
  • 批准号:
    1330488
  • 财政年份:
    2013
  • 资助金额:
    $ 30万
  • 项目类别:
    Continuing Grant
Structural and Dynamic Characterization of 2/2 Hemoglobins
2/2 血红蛋白的结构和动态表征
  • 批准号:
    0843439
  • 财政年份:
    2009
  • 资助金额:
    $ 30万
  • 项目类别:
    Standard Grant
Structural and Functional Characterization of Cyanoglobin
蓝红蛋白的结构和功能表征
  • 批准号:
    0804005
  • 财政年份:
    2007
  • 资助金额:
    $ 30万
  • 项目类别:
    Continuing Grant
Structural and Functional Characterization of Cyanoglobin
蓝红蛋白的结构和功能表征
  • 批准号:
    0349409
  • 财政年份:
    2004
  • 资助金额:
    $ 30万
  • 项目类别:
    Continuing Grant
Structural and Functional Characterization of Cyanoglobin
蓝红蛋白的结构和功能表征
  • 批准号:
    0091182
  • 财政年份:
    2001
  • 资助金额:
    $ 30万
  • 项目类别:
    Continuing Grant

相似国自然基金

Toward a general theory of intermittent aeolian and fluvial nonsuspended sediment transport
  • 批准号:
  • 批准年份:
    2022
  • 资助金额:
    55 万元
  • 项目类别:

相似海外基金

CAREER: Toward Smart Surface Acoustic Wave Devices with Gate-Tunability
职业:开发具有栅极可调谐性的智能表面声波器件
  • 批准号:
    2337069
  • 财政年份:
    2024
  • 资助金额:
    $ 30万
  • 项目类别:
    Continuing Grant
CAREER: Hybrid Surface Coating Toward Corrosion-Controlled Magnesium-Based Implants
职业:针对腐蚀控制镁基植入物的混合表面涂层
  • 批准号:
    2339911
  • 财政年份:
    2024
  • 资助金额:
    $ 30万
  • 项目类别:
    Continuing Grant
CAREER: A Bottom Up pAproach Toward Understanding the Sunlight Driven Mechanisms and Pathways for the Release of Metals from Petroleum.
职业:一种自下而上的方法来了解阳光驱动的机制和从石油中释放金属的途径。
  • 批准号:
    2340743
  • 财政年份:
    2024
  • 资助金额:
    $ 30万
  • 项目类别:
    Continuing Grant
The Information-Attention Tradeoff: Toward an Understanding of the Fundamentals of Online Attention
信息与注意力的权衡:了解在线注意力的基本原理
  • 批准号:
    2343858
  • 财政年份:
    2024
  • 资助金额:
    $ 30万
  • 项目类别:
    Continuing Grant
CAREER: Toward Power Delivery Network-aware Hardware Security
职业:迈向电力传输网络感知硬件安全
  • 批准号:
    2338069
  • 财政年份:
    2024
  • 资助金额:
    $ 30万
  • 项目类别:
    Continuing Grant
Toward Trustworthy Generative AI by Integrating Large Language Model with Knowledge Graph
通过将大型语言模型与知识图相结合,迈向可信赖的生成式人工智能
  • 批准号:
    24K20834
  • 财政年份:
    2024
  • 资助金额:
    $ 30万
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
Tuning Precision Fabricated Liquid Crystal Adsorbents - Toward Tailored Adsorption of Per- and Polyfluorinated Alkyl Substances
调整精密制造的液晶吸附剂 - 针对全氟和多氟烷基物质的定制吸附
  • 批准号:
    24K17729
  • 财政年份:
    2024
  • 资助金额:
    $ 30万
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
Toward next-generation flexible and interpretable deep learning: A novel evolutionary wide dendritic learning
迈向下一代灵活且可解释的深度学习:一种新颖的进化广泛的树突学习
  • 批准号:
    23K24899
  • 财政年份:
    2024
  • 资助金额:
    $ 30万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Mentored Excellence Toward Research and Industry Careers 2
指导卓越研究和行业职业 2
  • 批准号:
    2322416
  • 财政年份:
    2024
  • 资助金额:
    $ 30万
  • 项目类别:
    Standard Grant
Toward a more complete understanding of coastal upwelling dynamics
更全面地了解沿海上升流动力学
  • 批准号:
    2343008
  • 财政年份:
    2024
  • 资助金额:
    $ 30万
  • 项目类别:
    Standard Grant
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了