RUI: Regulation of the plant endocycle by F-BOX STRESS INDUCED 1

RUI：F-BOX STRESS INDUCED 1 对植物内循环的调节

• 批准号: 2035582
• 负责人: Bryan Thines
• 金额: $ 54.55万
• 依托单位: University of Puget Sound
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-01-15 至 2024-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2035582&HistoricalAwards=false
• 关键词: RUI; Regulation; plant; endocycle; BOX

A defining, and perpetually intriguing, feature of plants is their ability to thrive in highly dynamic and oftentimes stressful environments. However, many molecular mechanisms used by plants to increase resiliency under challenging conditions are unknown. This work investigates how the cell cycle and co-regulated nuclear processes are directly altered in response to external cues so that growth is optimized. Knowledge generated by this work can be applied to future crop development in a world impacted by climate change. This project will provide training opportunities and support for undergraduate students carrying out independent research projects. Funds will also support development of course-based undergraduate research experiences (CUREs), which will offer students in classes the opportunity to meaningfully engage with authentic research experiences. Students participating in CUREs tend to: 1) be more highly engaged and have positive views of science and lab work, 2) have a deeper understanding of the scientific process, and 3) have higher retention rates in the sciences. Importantly, CUREs make classes more inclusive and increase success by multiple measures for students of historically underrepresented groups. These authentic research experiences will be integrated into the lab portion Genetics classes at the University of Puget Sound and Survey of Biology classes taught at the Washington Corrections Center for Women as part of the Freedom Education Project Puget Sound (FEPPS), a college degree-granting prison education program. College-level prison education reduces recidivism by 43% and provides pathways to employment and/or further education after release.The endocycle, an alternative cell cycle where the nuclear genome replicates but the cell does not divide, increases plant fitness under stress and can be triggered by the environment. Little is known about mechanisms used by plants for stress-triggered endocycle entry, although the anaphase promoting complex/cyclosome (APC/C) promotes this switch. This work investigates endocycle entry by action of F-BOX STRESS INDUCED 1 (FBS1), a stress-inducible substrate adaptor in the ubiquitin 26S proteasome system (UPS) that selects protein targets for degradation. Preliminary data show that FBS1 interacts with multiple putative targets: 1) APC8, a core subunit of the APC/C, and 2) two WD40 repeat-like family proteins, which have been named FBS1 INTERACTING PROTEINs (FBIPs). This work investigates the hypothesis that FBS1 coordinates a stress-response by targeting APC8 (possibly a post-translationally modified form) and FBIPs for degradation. Molecular genetic approaches will lead to understanding of how FBS1 affects the cell cycle and plants under stress, and it will establish the biological roles of FBIPs. This work will establish in vivo protein interaction dynamics between FBS1 and its partners, and it will establish whether APC8 and FBIPs are true ubiquitylation targets in stress responses. Collectively, this project will lead to greater understanding of how plants use the UPS to induce plasticity in the cell cycle and co-occurring processes to match cellular processes with environmental conditions.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

植物的一个决定性的、永远吸引人的特征是它们能够在高度动态和经常充满压力的环境中茁壮成长。然而，植物在具有挑战性的条件下增加弹性的许多分子机制尚不清楚。这项工作研究了细胞周期和共同调节的核过程如何直接改变以响应外部信号，从而优化生长。这项工作产生的知识可以应用于受气候变化影响的世界的未来作物发展。本项目将为本科生开展自主研究项目提供培训机会和支持。资金还将支持基于课程的本科生研究体验（CUREs）的发展，这将为课堂上的学生提供有意义地参与真实研究体验的机会。参加CUREs的学生倾向于：1)对科学和实验室工作有更高的参与度和积极的看法，2)对科学过程有更深入的理解，3)在科学领域有更高的保留率。重要的是，CUREs使班级更具包容性，并通过多种措施为历史上代表性不足的群体的学生增加成功。作为普吉特海湾自由教育项目（FEPPS）的一部分，这些真实的研究经历将被整合到普吉特海湾大学遗传学课程的实验室部分和华盛顿妇女矫正中心讲授的生物学调查课程中，这是一个授予大学学位的监狱教育项目。大学水平的监狱教育减少了43%的再犯，并提供了就业和/或释放后继续教育的途径。内循环是细胞核基因组复制但细胞不分裂的另一种细胞周期，它增加了植物在压力下的适应性，并可由环境触发。尽管后期促进复合物/环小体（APC/C）促进了这种开关，但关于植物胁迫触发内循环进入的机制知之甚少。这项工作研究了F-BOX应力诱导1 （FBS1）的作用下进入内循环，这是一种应力诱导的底物适配器，在泛素26S蛋白酶体系统（UPS）中选择蛋白质目标进行降解。初步数据显示，FBS1与多个可能的靶点相互作用：1)APC/C的核心亚基APC8, 2)两个WD40重复样家族蛋白，它们被命名为FBS1相互作用蛋白（FBIPs）。本研究探讨了FBS1通过靶向APC8（可能是翻译后修饰的形式）和FBIPs降解来协调应激反应的假设。分子遗传学方法将有助于了解FBS1如何影响逆境下的细胞周期和植物，并将确定FBIPs的生物学作用。这项工作将建立体内FBS1及其伴侣蛋白相互作用动力学，并将确定APC8和FBIPs是否在应激反应中是真正的泛素化靶点。总的来说，这个项目将使人们更好地了解植物如何利用UPS来诱导细胞周期的可塑性，以及如何将细胞过程与环境条件相匹配。该奖项反映了美国国家科学基金会的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。