RAPID: Airborne CoV-2 Viability and Oxidation

RAPID：空气传播的 CoV-2 活力和氧化

• 批准号: 2037541
• 负责人: Barbara Turpin
• 金额: $ 20万
• 依托单位: University of North Carolina at Chapel Hill
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-01 至 2022-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2037541&HistoricalAwards=false
• 关键词: RAPID; Airborne; CoV; Viability; Oxidation

This RAPID project seeks to improve the understanding of the airborne transmission of (SARS) CoV-2, the virus behind the COVID-19 pandemic, via sub-micron airborne particles. Chamber experiments will be conducted to help determine how long (SARS) CoV-2 remains viable in sub-micron aerosol and how atmospheric oxidation, particle size, and other environmental conditions affect viability. The team includes well-established infectious-disease scientists, coronavirus microbiologists and aerosol scientists with a unique combination of techniques and instruments.The two objectives of this research are to: (1) Conduct controlled chamber experiments using a non-pathogenic model coronavirus embedded in submicron aerosol in the presence/absence of ozone (O3) and hydroxyl (OH) radicals (separately) to examine how viability is influenced by atmospheric oxidation; and (2) Measure the concentrations of (SARS) CoV-2 and non-pathogenic T3 bacteriophage in aerosol with a novel BioSpot sampler and a gelatin filter sampler in at least 2 locations (e.g., wastewater treatment plant, hospital, daycare, nursing home, grocery store, classroom), and if viable viruses are detected to examine the ability of airborne O3 or OH radical treatment to inactivate the airborne (SARS) CoV-2 virus. This research is expected to lead to a better understanding of the viability of coronaviruses in sub-micron aerosol. It is anticipated that this effort will provide information relevant to the improved design of mitigation measures and shared indoor/outdoor spaces that are more resilient to respiratory virus transmission and more conducive to protecting public health.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

这一快速项目旨在提高对新冠肺炎大流行背后的非典型肺炎冠状病毒-2通过亚微米空气中颗粒物通过空气传播的了解。将进行室内实验，以帮助确定(SARS)CoV-2在亚微米气溶胶中存活多长时间，以及大气氧化、颗粒大小和其他环境条件如何影响生存能力。该团队包括著名的传染病科学家、冠状病毒微生物学家和气溶胶科学家，他们拥有独特的技术和仪器组合。这项研究的两个目标是：(1)使用嵌入亚微米气溶胶的非致病模型冠状病毒，在存在/不存在臭氧(O3)和羟基(OH)自由基的情况下(分别)进行控制室实验，以研究大气氧化如何影响生存能力；和(2)在至少两个地点(如污水处理厂、医院、托儿所、疗养院、杂货店、教室)用新型BioSpot取样器和明胶过滤取样器检测气溶胶中SARS冠状病毒和非致病T3噬菌体的浓度，如果检测到活病毒，则检测空气中臭氧或羟基自由基处理对空气传播的(SARS)CoV-2病毒的灭活能力。这项研究有望更好地了解冠状病毒在亚微米气溶胶中的生存能力。预计这项工作将提供与改善缓解措施和共享室内/室外空间的设计相关的信息，这些措施对呼吸道病毒传播更具弹性，更有利于保护公众健康。该奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。