Biogenesis of beta-barrel proteins of the outer membranes of endosymbiotic organelles

内共生细胞器外膜β-桶蛋白的生物合成

• 批准号: 244855879
• 负责人: Professor Dr. Doron Rapaport
• 金额: --
• 依托单位: Interfakultäres Institut für Biochemie (IFIB)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2013
• 资助国家: 德国
• 起止时间: 2012-12-31 至 2022-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/244855879?language=en
• 关键词: Biogenesis; beta; barrel; proteins; outer

The outer membranes (OM) of the endosymbiotically derived organelles mitochondria and plastids constitute the communication platform between these organelles and the other cell compartments. Consequentially, the proteomes of these membranes were adopted during evolution to fulfill the imposed requirements ranging from exchange of solutes and macromolecules to lipid transfer and signal transduction. The vast majority of organeller proteins are encoded in the nucleus and synthesized on cytosolic ribosomes. A unique group of such organellar proteins are those with beta-barrel architecture that are found in the outer membranes of both organelles. The delivery of such OM proteins from the cytosol to their target membrane has to be tightly regulated especially in plant cells where the two organelles have to be supplied in parallel while keeping targeting specificity. The targeting factors that define this specificity are not yet known. We will identify such factors in yeast and plant cells, and will dissect their function in targeting of proteins in general and for the specific delivery of beta-barrel proteins to the correct organelle.

内共生细胞器、线粒体和质体的外膜构成了这些细胞器与其他细胞区室之间的通讯平台。因此，这些膜的蛋白质组在进化过程中被采用，以满足从溶质和大分子交换到脂质转移和信号转导的要求。绝大多数细胞器蛋白质在细胞核中编码并在胞质核糖体上合成。一组独特的细胞器蛋白质是在两个细胞器的外膜中发现的具有β桶结构的蛋白质。这种OM蛋白从胞质溶胶到其靶膜的递送必须受到严格调控，特别是在植物细胞中，其中两个细胞器必须平行供应，同时保持靶向特异性。定义这种特异性的靶向因素尚不清楚。我们将在酵母和植物细胞中识别这些因子，并将剖析它们在靶向蛋白质方面的功能，以及将β桶蛋白特异性递送到正确的细胞器。