Investigate the roles of endogenous salicylic acids in de novo organogenesis

研究内源性水杨酸在从头器官发生中的作用

• 批准号: 2039313
• 负责人: Li Yang
• 金额: $ 76.47万
• 依托单位: University of Georgia Research Foundation Inc
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-08-01 至 2024-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2039313&HistoricalAwards=false
• 关键词: Investigate; roles; endogenous; salicylic; acids

Plants have the unique and important ability to regenerate a new organ or even an entire seedling from a small piece of tissue. This process of tissue regeneration is a foundation for modern agriculture and horticulture. The propagation of many fruits and vegetables heavily relies on techniques involving tissue regeneration, such as grafting, tissue culture, and rooting from stem cuttings. These techniques provide a non-transgenic approach to produce chimera or improved cultivars and, eventually, to increase plants' vigor and resistance to pathogens. Thus, understanding the underlying mechanisms of tissue regeneration is important for food quality and security. This study investigates how plant regeneration occurs under the influence of microbial pathogens, which are constant threats to our agriculture. New molecular and cellular technologies will be used to examine the function of plant hormones in regulating the balance of regeneration and defense against pathogens. Knowledge obtained from this study will promote practical and scalable strategies to help regeneration in species where wound-healing and regeneration after cutting remains a critical bottleneck for propagation. The proposed project will also provide students at different levels with experiential learning of modern plant biotechnology. This study aims to elucidate the role of salicylic acid (SA) in wound-induced de novo Root Regeneration (DNRR), in which adventitious roots form on detached leaves. De novo organogenesis is a fascinating developmental process involving a drastic transition of cell fate. The molecular crosstalk between pathways governing biotic stress and regeneration represents a major knowledge gap. A general hypothesis is that antagonistic interaction between NPR family members fine-tunes the SA-mediated suppression of DNRR. The following specific aims will be addressed. Aim1: Determine the spatiotemporal patterns of SA response and accumulation during DNRR. Aim2: Test the hypothesis that NPR3/4 and NPR5/6 antagonistically regulate DNRR. Aim3: Characterize the function of an NPR4-TGA complex in DNRR. Completion of the proposed study will establish foundational knowledge about how endogenous SA suppresses the cell fate transition during DNRR. Characterizing the contribution of NPR family members and their interactions with TGA transcription factors will expand our knowledge of the distinct and overlapping function of NPR proteins in SA- mediated defense and development. Plant regeneration is an intriguing developmental process for students to observe and to gain hands-on experience. Select partners involved in undergraduate and K-12 education will participate in this study to enhance the experiential learning of plant development, especially for underrepresented groups in STEM education.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

植物具有独特而重要的能力，可以从一小块组织再生一个新器官，甚至是整个幼苗。这种组织再生过程是现代农业和园艺的基础。许多水果和蔬菜的繁殖在很大程度上依赖于涉及组织再生的技术，如嫁接、组织培养和茎插生根。这些技术提供了一种非转基因的方法来生产嵌合体或改良品种，并最终增加植物的活力和对病原体的抗性。因此，了解组织再生的基本机制对食品质量和安全至关重要。这项研究调查了植物再生如何在微生物病原体的影响下发生，这些病原体是对我们农业的持续威胁。新的分子和细胞技术将用于研究植物激素在调节再生和防御病原体的平衡中的功能。从这项研究中获得的知识将促进实用和可扩展的策略，以帮助再生的物种，伤口愈合和再生后切割仍然是一个关键的瓶颈繁殖。建议的计划亦会为不同程度的学生提供现代植物生物技术的体验学习。 本研究旨在阐明水杨酸（SA）在创伤诱导离体叶片再生不定根中的作用。从头器官发生是一个迷人的发育过程，涉及细胞命运的急剧转变。控制生物胁迫和再生的途径之间的分子串扰代表了一个主要的知识空白。一般假设是NPR家族成员之间的拮抗相互作用微调SA介导的DNRR抑制。将处理以下具体目标。目的1：确定DNRR期间SA响应和累积的时空模式。目的2：检验NPR 3/4和NPR 5/6拮抗调节DNRR的假设。目的3：表征DNRR中NPR 4-TGA复合物的功能。完成拟议的研究将建立关于内源性SA如何抑制DNRR期间细胞命运转变的基础知识。表征NPR家族成员的贡献及其与TGA转录因子的相互作用将扩展我们对NPR蛋白在SA介导的防御和发育中的独特和重叠功能的认识。植物再生是一个有趣的发展过程，让学生观察和获得实践经验。参与本科和K-12教育的精选合作伙伴将参与这项研究，以加强植物开发的体验式学习，特别是对STEM教育中代表性不足的群体。该奖项反映了NSF的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

The diversity of salicylic acid biosynthesis and defense signaling in plants: Knowledge gaps and future opportunities

植物中水杨酸生物合成和防御信号的多样性：知识差距和未来机遇

• DOI: 10.1016/j.pbi.2023.102349
• 发表时间: 2023
• 期刊: Current Opinion in Plant Biology
• 影响因子: 9.5
• 作者: Ullah, Chhana;Chen, Yen-Ho;Ortega, María A.;Tsai, Chung-Jui
• 通讯作者: Tsai, Chung-Jui