Design, Implementation, and Evaluation of a Gene Editing Technology Toolkit for Undergraduate Education

本科教育基因编辑技术工具包的设计、实现和评估

• 批准号: 2044385
• 负责人: Hooi Lynn Kee
• 金额: $ 30万
• 依托单位: Stetson University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-02-15 至 2025-01-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2044385&HistoricalAwards=false
• 关键词: Design; Implementation; Evaluation; Gene; Editing

This project aims to serve the national interest by educating biology students in the application, use, and ethics of a novel gene editing technology. Clustered Regularly Interspersed Short Palindromic Repeats (CRISPR) technology has emerged as a rapid and easy to use gene editing technique. CRISPR technology, which was recognized with a Nobel Prize in 2020, is making an impact in medicine, agriculture, food production, public policy, and business, as well as in popular media. Given the broad reach of CRISPR, there is an increased need and urgency for undergraduate students to learn about and consider how to use the technology appropriately. The project plans to build instructional modules on CRISPR technology for both introductory and advanced biology students. These modules will be designed to create curricular experiences that are novel, relevant, and accessible for students as well as to increase faculty understanding of CRISPR technology. By implementing these modules with both STEM and non-STEM majors, this work should help develop informed citizens who can confront the societal implications of CRISPR and other gene editing technologies. The overall goal of the proposed project is to develop, implement, and evaluate both dry-lab and wet-lab educational experiences framed around gene editing using CRISPR. The toolkit to be developed in the project will include a series of dry-lab Case-Based Learning modules that focus on CRISPR editing of genes and that will engage students through computer-based DNA sequence analysis, manipulation, and modeling. Wet-lab experiences will include a Course-Based Undergraduate Research Experience that is framed around using CRISPR technology in painted lady butterflies to study butterfly wing and body patterning and development. Bioethical training will be built into both curricular activities to promote the students’ understanding of the societal implications of gene editing. The project team will evaluate the impacts of the new curricula on student learning, scientific design and process skills, ethical reasoning, and post-course attitudes and activities. The project is intended to provide broad training and support of educators by disseminating curricular materials and approaches through virtual instructional workshops, conference presentations, publications, and the development of a community of practice for CRISPR technology educators. The NSF IUSE: EHR Program supports research and development projects to improve the effectiveness of STEM education for all students. Through the Engaged Student Learning track, the program supports the creation, exploration, and implementation of promising practices and tools.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该项目旨在通过教育生物学学生新型基因编辑技术的应用、使用和伦理来服务国家利益。 成簇规律散布的短回文重复序列 (CRISPR) 技术已成为一种快速且易于使用的基因编辑技术。 CRISPR 技术荣获 2020 年诺贝尔奖，正在对医学、农业、食品生产、公共政策、商业以及大众媒体产生影响。 鉴于 CRISPR 的广泛应用，本科生学习并考虑如何正确使用该技术的需求和紧迫性越来越大。 该项目计划为初级和高级生物学学生构建 CRISPR 技术的教学模块。 这些模块旨在为学生创造新颖、相关且易于理解的课程体验，并提高教师对 CRISPR 技术的理解。 通过在 STEM 和非 STEM 专业学生中实施这些模块，这项工作应该有助于培养知识渊博的公民，让他们能够面对 CRISPR 和其他基因编辑技术的社会影响。 该项目的总体目标是开发、实施和评估围绕使用 CRISPR 进行基因编辑的干实验室和湿实验室教育体验。 该项目中将开发的工具包将包括一系列基于案例的干燥实验室学习模块，这些模块专注于基因的 CRISPR 编辑，并通过基于计算机的 DNA 序列分析、操作和建模吸引学生。 湿实验室体验将包括基于课程的本科生研究体验，该体验的框架是在彩绘蝴蝶中使用 CRISPR 技术来研究蝴蝶翅膀和身体的图案和发育。 生物伦理培训将纳入这两项课程活动，以促进学生了解基因编辑的社会影响。 项目团队将评估新课程对学生学习、科学设计和过程技能、道德推理以及课后态度和活动的影响。 该项目旨在通过虚拟教学研讨会、会议演讲、出版物以及为 CRISPR 技术教育工作者开发实践社区来传播课程材料和方法，从而为教育工作者提供广泛的培训和支持。 NSF IUSE：EHR 计划支持研究和开发项目，以提高所有学生 STEM 教育的有效性。 通过参与学生学习轨道，该计划支持有前途的实践和工具的创建、探索和实施。该奖项反映了 NSF 的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

CRISPR in butterflies: An undergraduate lab experience to inactivate wing patterning genes during development

蝴蝶中的 CRISPR：在发育过程中灭活翅膀图案基因的本科生实验室经验

• DOI: 10.1002/bmb.21669
• 发表时间: 2022
• 期刊: Biochemistry and Molecular Biology Education
• 影响因子: 1.4
• 作者: Thulluru, Aamani;Saad, Luisa;Nagah Abdou, Yasmin;Martin, Arnaud;Kee, Hooi Lynn
• 通讯作者: Kee, Hooi Lynn

Bringing revolutionary gene editing techniques to undergraduates

为本科生带来革命性的基因编辑技术

• DOI: 10.33424/futurum442
• 发表时间: 2023
• 期刊: Futurum Careers
• 作者: Challa, Anil;Kee, Lynn
• 通讯作者: Kee, Lynn

Investigating butterfly wing color gene using CRISPR technology

利用 CRISPR 技术研究蝴蝶翅膀颜色基因

• DOI: 10.37590/able.v43.art11
• 发表时间: 2023
• 期刊: Advances in Biology Laboratory Education
• 作者: Kee, HL