CAREER: Empirically evaluating the genomic consequences of assisted migration in heterogeneous environments

职业：根据经验评估异质环境中辅助迁移的基因组后果

• 批准号: 2045643
• 负责人: Nicholas Kooyers
• 金额: $ 99.73万
• 依托单位: University of Louisiana at Lafayette
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-04-01 至 2026-03-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2045643&HistoricalAwards=false
• 关键词: CAREER; Empirically; evaluating; genomic; consequences

Responding to anthropogenic climate change is both a sociological and a scientific issue. Current studies suggest that many species are now lagging behind changing climatic conditions and are doing poorly relative to past conditions. Determining which species are impacted, how fast species are declining, and evaluating potential solutions to counter declines are key goals. One potential solution is assisted migration, where individuals from a geographically-distant population that are better adapted to the changing conditions are intentionally introduced into a focal population. Assisted migration is controversial because there are often unknown consequences when introducing new variation into a population, and because it has largely been untested via controlled experiments. This award experimentally addresses the consequences of assisted migration in a widespread plant that is threatened in a small portion of its distribution. Seeds and seedlings from populations already adapted to contemporary conditions will be introduced into a subset of focal populations. Comparisons of traits, genomes, and population success between these experimental populations and nearby control populations will provide the best empirical data collected to date on the efficiency and potential utility of assisted migration. This award also addresses the need for greater public understanding of climate change across the U.S. by providing secondary education teachers with the skills, lesson plans, and information necessary to address this issue in the classroom. This award funds the creation of a dynamic game-like climate change outreach module, a graduate student-led science outreach team to go into secondary education classrooms, and a seminar course for aspiring STEM teachers to instruct teachers how to address contentious issues. The assisted migration experiment awarded here evaluates three main questions: how much introgression occurs across the genome following the introduction and how long does it persist? Does introgression shift trait distributions or enable transgressive phenotypes to emerge? Do introductions improve fitness of the native populations in normal or extreme years? This project addresses these questions by leveraging the genomic resources of a model organism, Mimulus guttatus. Preliminary data indicate that elevation-matched California populations have higher fitness than native Oregon Cascades populations, and that Oregon Cascades populations are in danger of extirpation in extreme climatic years. This project introduces California populations into a subset of twelve populations that have been followed for the last three years. The amount and timing of introgression following introduction will be surveyed by using low coverage whole genome sequencing to track genetic variants specific to the introduced populations over three years post-introduction. A multi-year common garden experiment with seeds collected each year during the experiment will be used to evaluate how phenotypes and fitness changes following assisted migration. While this experiment provides important value to the conservation community, it also will provide novel insights into how evolutionary forces interact within a natural population to remove and maintain polymorphism on rapid ecological timescales. This award funds training for five undergraduate researchers and a graduate student recruited primarily through diversity initiatives as well as public dissemination via established outreach events targeting multiple age groups.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

应对人为气候变化的反应既是社会学和科学问题。目前的研究表明，现在许多物种落后于气候条件的变化，并且相对于过去的条件而言的情况差。确定哪些物种受到影响，物种下降的速度以及评估反向下降的潜在解决方案是关键目标。一种潜在的解决方案是辅助迁移，在该迁移中，从地理上居民中更好地适应不断变化的条件的人有意将其引入焦点人群中。辅助迁移是有争议的，因为在将新变化引入人群时通常会产生未知的后果，并且由于它在很大程度上通过受控实验未经测试。该奖项通过实验解决了在其分布的一小部分威胁的广泛工厂中辅助移民的后果。来自已经适应当代条件的种群的种子和幼苗将被引入焦点种群的一部分。这些实验人群与附近的控制人群之间的性状，基因组和人口成功的比较将提供迄今为止收集的最佳经验数据，迄今为止，辅助迁移的效率和潜在效用。该奖项还涉及通过为中等教育教师提供在课堂上解决此问题所需的技能，课程计划和信息，以对美国整个美国的气候变化有更深入的了解。该奖项为创建一个充满活力的游戏式气候变化外展模块的创建资金，由研究生主导的科学外展团队进入中学教育教室，并为有抱负的STEM老师提供一个研讨会课程，以指导老师如何解决有争议的问题。这里授予的辅助迁移实验评估了三个主要问题：引言后整个基因组中发生了多少渗入，以及它持续了多长时间？渗入的转移特征分布或使侵犯表型出现吗？介绍是否可以改善正常或极端年份本地人口的适应性？该项目通过利用模型生物Mimulus Guttatus的基因组资源来解决这些问题。初步数据表明，高程匹配的加利福尼亚人口的健身比俄勒冈州级联级联人口高，并且俄勒冈州级联人口在极端气候时期内有灭绝的危险。该项目将加利福尼亚州的人口引入了过去三年中遵循的十二个人口。引入之后的渗入的数量和时机将通过使用低覆盖范围的整个基因组测序来跟踪引入后三年引入人群特异性人群的遗传变异体进行调查。每年在实验期间收集的种子进行的多年普通花园实验将用于评估辅助迁移后表型和健身的变化。尽管该实验为保护界提供了重要的价值，但它还将提供新的见解，以了解在自然人群中如何相互作用以消除和维持快速生态时刻度的多态性。该奖项是针对五名本科研究人员和一名研究生培训，主要是通过多样性倡议以及通过针对多个年龄段的既定外展活动进行招聘的研究生培训。这项奖项反映了NSF的法定任务，并被认为是值得通过基金会的知识分子和更广泛影响的评估来通过评估来获得支持的。

项目成果

Life history and chemical defense interact to drive patterns of local adaptation in an annual monkeyflower

生活史和化学防御相互作用，驱动一年生猴花的局部适应模式

• 发表时间: 2023
• 期刊: Evolution
• 影响因子: 3.3
• 作者: Scharnagl, Anna;Genung, Mark A.;Holeski, Liza M.;Kooyers, Nicholas J.
• 通讯作者: Kooyers, Nicholas J.

Unique drought resistance strategies occur among monkeyflower populations spanning an aridity gradient

跨越干旱梯度的猴花种群中存在独特的抗旱策略

• DOI: 10.1002/ajb2.16207
• 发表时间: 2023
• 期刊: American Journal of Botany
• 影响因子: 3
• 作者: FitzPatrick, Joshua A.;Doucet, Braden I.;Holt, Stacy D.;Patterson, Courtney M.;Kooyers, Nicholas J.
• 通讯作者: Kooyers, Nicholas J.