CAREER: Exploring the windows of selection for insecticide resistance evolution

职业：探索杀虫剂抗性进化的选择窗口

• 批准号: 2047572
• 负责人: Silvie Huijben
• 金额: $ 96.07万
• 依托单位: Arizona State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-08-01 至 2026-10-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2047572&HistoricalAwards=false
• 关键词: CAREER; Exploring; windows; selection; insecticide

The evolution of resistance against herbicides, fungicides, pesticides, antimicrobials, antivirals, and anticancer drugs is a major global challenge. To develop effective resistance management strategies, it is important to understand how the different combinations and concentrations affect the evolution of resistance. This knowledge could be used to understand how resistance evolution changes under varying exposures or as concentrations wane over time. The aim of this project is to fill the gap in this knowledge by focusing on insecticide resistance evolution in mosquitoes as a model system. A comprehensive evolutionary framework of insecticide resistance evolution will be developed by measuring the selection acting upon resistant and susceptible mosquitoes under different insecticide combinations and concentrations. Furthermore, with problems as complex as resistance evolution that is predicted to continue globally in the foreseeable future, a diverse workforce is urgently needed. This project will develop research and inquiry-based experiences for undergraduate and K-12 students around insecticide resistance evolution. The focus will be on students from lower socio-economic or underrepresented backgrounds to bolster student engagement in science and expose a diverse group of students to the process of science.Resistance evolution is driven by increased survival of resistant organisms in a xenobiotic environment (an environment with pesticides, antibiotics, or other "-cides") relative to susceptible organisms. The window of selection is the range of concentrations at which differential mortality is observed and is used as a framework to identify concentrations at which selection occurs, or identify the length of time this window is open at inevitably waning concentrations. Often missing from this framework is the role of interspecific competition at concentrations for which both strains survive: at low concentrations. The research goal is to test the hypothesis that the window of selection contains one or more sub-windows of competition, followed by a window of negative selection. The specific aims are to: 1) establish the selection coefficient of insecticide resistance alleles at different doses within the window of selection, 2) determine the magnitude of fitness costs associated with insecticide resistance alleles and 3) determine selection following various resistance management strategies using experimental and mathematical approaches.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

对除草剂、杀菌剂、杀虫剂、抗菌剂、抗病毒药物和抗癌药物的耐药性演变是一项重大的全球性挑战。为了制定有效的耐药性管理策略，了解不同的组合和浓度如何影响耐药性的演变是很重要的。这一知识可用于了解在不同暴露或浓度随时间下降时耐药性进化如何变化。该项目的目的是通过将蚊子的杀虫剂抗性进化作为一个模型系统来填补这方面的知识空白。通过测量不同杀虫剂组合和浓度下对抗性和易感蚊子的选择，建立杀虫剂抗性进化的综合进化框架。此外，由于预计在可预见的未来，全球范围内的耐药性演变等复杂问题将继续存在，因此迫切需要一支多样化的工作队伍。该项目将为本科生和K-12学生提供关于杀虫剂抗性进化的研究和探究性经验。重点将放在社会经济地位较低或代表性不足的背景的学生身上，以加强学生对科学的参与，并使不同群体的学生接触到科学的过程。相对于易感生物，耐药生物在外源环境（有杀虫剂、抗生素或其他“杀菌剂”的环境）中生存的增加推动了耐药性进化。选择窗口是观察到不同死亡率的浓度范围，并用作确定发生选择的浓度的框架，或确定在不可避免的浓度下降时该窗口打开的时间长度。在这一框架中经常缺失的是两种菌株在低浓度下生存的浓度下的种间竞争的作用。研究的目的是检验选择窗口包含一个或多个竞争子窗口，然后是一个消极选择窗口的假设。具体目的是：1)建立选择窗口内不同剂量杀虫剂抗性等位基因的选择系数；2)确定与杀虫剂抗性等位基因相关的适应度成本大小；3)采用实验和数学方法确定不同抗性管理策略下的选择。该奖项反映了美国国家科学基金会的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Topical Application Bioassay to Quantify Insecticide Toxicity for Mosquitoes and Fruit Flies

• DOI: 10.3791/63391
• 发表时间: 2022-01-01
• 期刊: JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
• 影响因子: 1.2
• 作者: Jensen, Brook M.;Althoff, Rachel A.;Huijben, Silvie
• 通讯作者: Huijben, Silvie