CAREER: Discovering hidden drivers of rhizosphere symbiosis and parasitism
职业:发现根际共生和寄生的隐藏驱动因素
基本信息
- 批准号:2047684
- 负责人:
- 金额:$ 50万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Continuing Grant
- 财政年份:2021
- 资助国家:美国
- 起止时间:2021-06-01 至 2026-05-31
- 项目状态:未结题
- 来源:
- 关键词:
项目摘要
Public Abstract – Proposal IOS-2047684Title: CAREER: Hidden drivers of parasitism and symbiosis in the rhizosphereThis project focuses on plant-parasitic nematodes, tiny worms that feed on plants, causing an estimated 25% of crop yield loss globally and costing $100 billion annually. Naturally occurring bacteria have been discovered living within these worms that may drive their survival and direct their devastating impacts on plants. These bacteria, present in some of the most damaging nematodes, are related to bacteria currently used to control mosquito-borne diseases, suggesting they may hold promise for agricultural control strategies that reduce the use of toxic chemicals to control nematodes. This project uses global sampling, DNA and RNA sequencing, computer software development, and novel experiments to uncover the function of these widespread bacteria and bridge traditionally separated fields of plant pathology, nematology, and microbiology to overcome significant roadblocks to understanding rhizosphere ecology. This project also examines how stressors such as climate change and land use affect the interactions between these bacteria, nematodes, and plants. This project will have societal and educational impacts through integrating research activities performed by an interdisciplinary science program in bioinformatics at the hosting Hispanic Serving Institution with national research-outreach programs centered on citizen science and rhizosphere biomodelling, and a local peer-mentoring program in genomics for high school students targeting traditionally underrepresented groups.Plant-parasitic nematodes (PPNs) are ubiquitous in soils, causing significant losses to agricultural crops and impacts on plants across terrestrial ecosystems. Recent work shows these nematodes host bacterial endosymbionts, including Wolbachia and Cardinium, which are well-known in insects to play a critical role in driving their host’s biology as either reproductive parasites or mutualists. Yet, the role of these endosymbionts in PPNs is unknown. This CAREER project seeks to uncover the dynamics driving the tripartite interactions between these endosymbionts, PPNs, and plants. Research activities will (1) determine the ecological and evolutionary forces acting on PPN endosymbionts globally across natural populations through shotgun metagenomics, population genomics, database mining, and bioinformatics; (2) directly test endosymbiont impacts on host nematode fitness, phenotype, and plant parasitism through symbiont-clearing experiments on root-cultures, plants, and transparent soils, using host-symbiont gene expression analysis; and (3) investigate the dual effects of PPN endosymbionts and stressors such as climate and land use change using computational models and transplanted plant communities under temperature stress. These experiments will test the hypothesis that PPN endosymbionts drive the tripartite dynamics through controlling host reproduction or providing iron/heme or lipids and explore molecular targets for biocontrol. The project integrates research, education, and outreach objectives by developing and incorporating Citizen Science-based sampling, an undergraduate Bioinformatics Challenge program, and a summer high school Genomics Scholar program to engage and train underrepresented groups in this research. Outcomes are expected to form a new foundational understanding of these rhizosphere interactions, generate bioinformatics software, and improve training in multi-omics.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
公开摘要-提案IOS-2047684标题:职业:根际寄生和共生的隐藏驱动因素该项目专注于植物寄生线虫,这是一种以植物为食的微小蠕虫,造成全球约25%的作物产量损失,每年造成1000亿美元的损失。已经发现生活在这些蠕虫体内的自然产生的细菌,这些细菌可能会推动它们的生存,并直接对植物产生毁灭性的影响。这些细菌存在于一些最具破坏性的线虫中,与目前用于控制蚊媒疾病的细菌有关,这表明它们可能为减少使用有毒化学品控制线虫的农业控制策略带来希望。该项目使用全球采样、DNA和RNA测序、计算机软件开发和新颖的实验来揭示这些广泛存在的细菌的功能,并架起植物病理学、线虫学和微生物学传统上相互分离的领域的桥梁,以克服理解根际生态学的重大障碍。该项目还研究了气候变化和土地利用等应激源如何影响这些细菌、线虫和植物之间的相互作用。该项目将通过将主办拉美裔服务机构的生物信息学跨学科科学计划开展的研究活动与以公民科学和根际生物建模为中心的国家研究推广计划以及针对传统上代表不足的群体的高中生基因组学同行指导计划相结合,产生社会和教育影响。植物寄生线虫(PPN)在土壤中普遍存在,给农作物造成重大损失,并对陆地生态系统中的植物造成影响。最近的研究表明,这些线虫拥有细菌内共生体,包括沃尔巴克氏菌和卡迪纳菌,这些细菌在昆虫中作为生殖寄生虫或互助者在推动宿主生物学方面发挥着关键作用。然而,这些内共生菌在PPN中的作用尚不清楚。这个职业项目试图揭示推动这些内共生体、PPN和植物之间三方相互作用的动力。研究活动将:(1)通过鸟枪式元基因组学、种群基因组学、数据库挖掘和生物信息学,确定全球自然种群中PPN内共生菌所受的生态和进化力量;(2)通过根培养物、植物和透明土壤上的共生体清除实验,利用宿主-共生体基因表达分析,直接测试内共生菌对宿主线虫适合度、表型和植物寄生的影响;以及(3)利用计算模型和温度胁迫下移植的植物群落,研究PPN内共生菌和应激源(如气候和土地利用变化)的双重影响。这些实验将检验PPN内共生菌通过控制寄主繁殖或提供铁/血红素或脂类来驱动三方动力学的假设,并探索生物控制的分子靶标。该项目通过开发和整合基于公民科学的采样、本科生生物信息学挑战计划和暑期高中基因组学奖学金计划来整合研究、教育和推广目标,以吸引和培训未被充分代表的群体参与这项研究。预计结果将形成对这些根际相互作用的新的基础性理解,生成生物信息学软件,并改进多项功能的培训。该奖项反映了NSF的法定使命,并通过使用基金会的智力优势和更广泛的影响审查标准进行评估,被认为值得支持。
项目成果
期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Amanda Brown其他文献
Digital creativity support for original journalism
为原创新闻提供数字创意支持
- DOI:
10.1145/3386526 - 发表时间:
2020 - 期刊:
- 影响因子:22.7
- 作者:
N. Maiden;K. Zachos;Amanda Brown;Dimitris Apostolou;Balder Holm;Lars Nyre;A. Tonheim;Arend van den Beld - 通讯作者:
Arend van den Beld
Becoming a high-performing team.
成为一支高绩效团队。
- DOI:
- 发表时间:
2020 - 期刊:
- 影响因子:0
- 作者:
Megan Stevens;Amanda Brown;J. Vaughn;H. Clancy;M. Kalman - 通讯作者:
M. Kalman
Patients with Dyslipidemia and Heart Failure Affected by Non-Alcoholic Fatty Liver Disease Have higher Mortality and Worse Clinical Outcomes, Nation-Wide Study
- DOI:
10.1016/j.jacl.2023.05.030 - 发表时间:
2023-07-01 - 期刊:
- 影响因子:
- 作者:
Leonid Khokhlov;Usha Thapa;Sarthak Aryal;Leanne Pereira;Michael Fatuyi;Fatima Hussain;Mehnaaz Ali;Amr Aboelnasr;Roman Khokhlov;Mohamed Labban;Michael Eerhart;Amanda Brown;Kamal Shemisa - 通讯作者:
Kamal Shemisa
055 – Embedding Evidence-Based Changes: Water Occlusive Barriers in Venous Line Care?
- DOI:
10.1016/j.pedn.2009.04.003 - 发表时间:
2009-04-01 - 期刊:
- 影响因子:
- 作者:
Amanda Brown - 通讯作者:
Amanda Brown
The Way She Looked the Day She Died: Vernacular Photography, Memory, & Death
她去世那天的样子:乡土摄影、记忆、
- DOI:
- 发表时间:
2013 - 期刊:
- 影响因子:0
- 作者:
Amanda Brown - 通讯作者:
Amanda Brown
Amanda Brown的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Amanda Brown', 18)}}的其他基金
Examining an On-line, International Exchange Professional Development Program for High School Teachers
审查高中教师在线国际交流专业发展计划
- 批准号:
2201087 - 财政年份:2022
- 资助金额:
$ 50万 - 项目类别:
Continuing Grant
相似海外基金
CDS&E: Immersive Virtual Reality for Discovering Hidden Chemical Information and Improving Multivariate Modeling and Predication
CDS
- 批准号:
2305020 - 财政年份:2023
- 资助金额:
$ 50万 - 项目类别:
Standard Grant
Discovering Hidden Planets and Testing Our Planet Formation Theories
发现隐藏的行星并测试我们的行星形成理论
- 批准号:
569644-2022 - 财政年份:2022
- 资助金额:
$ 50万 - 项目类别:
Alexander Graham Bell Canada Graduate Scholarships - Doctoral
EAGER: Discovering the hidden functional trait diversity of Bees (Apoidea) and predicting functional diversity impacts on rendered ecosystem services
EAGER:发现蜜蜂(Apoidea)隐藏的功能特征多样性并预测功能多样性对所提供的生态系统服务的影响
- 批准号:
2132730 - 财政年份:2021
- 资助金额:
$ 50万 - 项目类别:
Standard Grant
Development of plant conservation genetics by discovering hidden linages in New Caledonia using population genomics analysis
利用群体基因组学分析发现新喀里多尼亚隐藏的谱系,发展植物保护遗传学
- 批准号:
21KK0104 - 财政年份:2021
- 资助金额:
$ 50万 - 项目类别:
Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))
Discovering hidden control elements for crop improvement
发现作物改良的隐藏控制因素
- 批准号:
DE200101748 - 财政年份:2020
- 资助金额:
$ 50万 - 项目类别:
Discovery Early Career Researcher Award
DISCOVERING HIDDEN ABILITIES (DHA): BUILDING ASSESSMENT TOOLS FOR CHILDREN WITH ATYPICAL DEVELOPMENT.
发现隐藏能力 (DHA):为非典型发育儿童构建评估工具。
- 批准号:
1956714 - 财政年份:2017
- 资助金额:
$ 50万 - 项目类别:
Studentship
III: Small: Discovering Hidden Semantics from Spatio-temporal Sensed Data
III:小:从时空感知数据中发现隐藏语义
- 批准号:
1527984 - 财政年份:2015
- 资助金额:
$ 50万 - 项目类别:
Standard Grant
NetSE: Medium: Discovering Hyperbolic Metric Spaces Hidden beneath the Internet and Other Complex Networks
NetSE:中:发现隐藏在互联网和其他复杂网络之下的双曲度量空间
- 批准号:
1441828 - 财政年份:2014
- 资助金额:
$ 50万 - 项目类别:
Standard Grant
Modelling and discovering complex interaction relations hidden in group behaviours in businesses, online and social communities
建模和发现隐藏在企业、在线和社交社区中群体行为中的复杂交互关系
- 批准号:
DP130102691 - 财政年份:2013
- 资助金额:
$ 50万 - 项目类别:
Discovery Projects
Discovering signals of selection in cancer mutations with Hidden Markov Models
使用隐马尔可夫模型发现癌症突变的选择信号
- 批准号:
219638969 - 财政年份:2012
- 资助金额:
$ 50万 - 项目类别:
Research Fellowships