Understanding the protein composition and dynamics of the nuclear envelope in plants

了解植物核膜的蛋白质组成和动力学

• 批准号: 2049931
• 负责人: Yangnan Gu
• 金额: $ 95万
• 依托单位: University of California-Berkeley
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-01-15 至 2024-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2049931&HistoricalAwards=false
• 关键词: Understanding; protein; composition; dynamics; nuclear

The nuclear envelope (NE) isolates and organizes the genome. It selectively accumulates proteins that carry out vital cellular functions and represents the hallmark of eukaryotic cells. Despite the increased appreciation of the NE as a crucial structural and signaling platform, knowledge on the composition and function of NE proteins is limited, particularly in plants, which significantly limits our understanding of plant NE function and its critical influence on various aspects of plant physiology and cell biology. This project aims to assemble the inventory of NE membrane proteins in the model organism Arabidopsis as a critical step towards a comprehensive understanding of the plant NE constituents and functions. In addition, this project will investigate molecular mechanisms that regulate the NE protein homeostasis through specialized proteolytic system. This research will generate valuable NE-associated protein databases and innovative protein identification tools for the plant and NE biology research community and deeply engage STEM and URM undergraduates in cutting-edge training in genetics, proteomics, cell biology, and molecular biology.Recent studies discovered a range of novel plant NE components and their significance in diverse biological processes, providing new insights into conserved eukaryotic NE functions as well as NE-associated mechanisms unique to plants. This project will deploy the newly developed proximity labeling technology coupled with label-free quantitative mass spectrometry to make rapid advances in establishing a precise and comprehensive view of the protein landscape in the plant NE. Using a combined genetic, proteomic, and cell biological approach, this project will also define key regulatory components of a newly identified inner nuclear membrane-associated protein degradation pathway that is critical in maintaining the NE function, adding a new branch to the organelle-specific membrane-associated proteolytic system in higher eukaryotes. This research promises to significantly advance our understanding of fundamental biological principles underlying the nuclear membrane and nuclear function.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

核膜（NE）分离并组织基因组。它选择性地积累执行重要细胞功能并代表真核细胞标志的蛋白质。尽管人们越来越认识到 NE 作为重要的结构和信号平台，但对 NE 蛋白的组成和功能的了解仍然有限，特别是在植物中，这极大地限制了我们对植物 NE 功能及其对植物生理学和细胞生物学各个方面的关键影响的理解。该项目旨在收集模式生物拟南芥中的 NE 膜蛋白库存，作为全面了解植物 NE 成分和功能的关键一步。此外，该项目将研究通过专门的蛋白水解系统调节 NE 蛋白稳态的分子机制。这项研究将为植物和 NE 生物学研究界生成有价值的 NE 相关蛋白质数据库和创新的蛋白质鉴定工具，并使 STEM 和 URM 本科生深入参与遗传学、蛋白质组学、细胞生物学和分子生物学的前沿培训。最近的研究发现了一系列新颖的植物 NE 成分及其在不同生物过程中的重要性，为保守的真核 NE 功能提供了新的见解。 以及植物特有的 NE 相关机制。该项目将部署新开发的邻近标记技术与无标记定量质谱相结合，以在建立植物 NE 中蛋白质景观的精确和全面视图方面取得快速进展。该项目还将采用遗传、蛋白质组学和细胞生物学相结合的方法，定义新发现的内核膜相关蛋白降解途径的关键调控成分，该途径对于维持 NE 功能至关重要，为高等真核生物中细胞器特异性膜相关蛋白水解系统添加一个新分支。这项研究有望显着增进我们对核膜和核功能的基本生物学原理的理解。该奖项反映了 NSF 的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Dynamic nucleoskeleton in stress

应力下的动态核骨架

• DOI: 10.1038/s41477-023-01458-1
• 发表时间: 2023
• 期刊: Nature Plants
• 影响因子: 18
• 作者: Fang, Yiling;Gu, Yangnan
• 通讯作者: Gu, Yangnan

Nuclear transport receptor KA120 regulates molecular condensation of MAC3 to coordinate plant immune activation

• DOI: 10.1016/j.chom.2023.08.015
• 发表时间: 2023-10-11
• 期刊: CELL HOST & MICROBE
• 影响因子: 30.3
• 作者: Jia，Min;Chen，Xuanyi;Gu，Yangnan
• 通讯作者: Gu，Yangnan

A karyopherin constrains nuclear activity of the NLR protein SNC1 and is essential to prevent autoimmunity in Arabidopsis

• DOI: 10.1016/j.molp.2021.06.011
• 发表时间: 2021-10-04
• 期刊: MOLECULAR PLANT
• 影响因子: 27.5
• 作者: Jia, Min;Shen, Xueqi;Gu, Yangnan
• 通讯作者: Gu, Yangnan

PNET2 is a component of the plant nuclear lamina and is required for proper genome organization and activity

• DOI: 10.1016/j.devcel.2021.11.002
• 发表时间: 2022-01-10
• 期刊: DEVELOPMENTAL CELL
• 影响因子: 11.8
• 作者: Tang, Yu;Dong, Qianli;Gu, Yangnan
• 通讯作者: Gu, Yangnan

A loyal “G”uard

忠诚的“G”后卫

• DOI: 10.1016/j.chom.2022.09.016
• 发表时间: 2022
• 期刊: Cell Host & Microbe
• 影响因子: 30.3
• 作者: Kenneally, Ryan;Gu, Yangnan
• 通讯作者: Gu, Yangnan