Subcellular interrogation of muscle dynamics with integrated optoelectronic arrays

利用集成光电阵列对肌肉动力学进行亚细胞询问

基本信息

  • 批准号:
    2055457
  • 负责人:
  • 金额:
    $ 36万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Standard Grant
  • 财政年份:
    2021
  • 资助国家:
    美国
  • 起止时间:
    2021-08-01 至 2024-07-31
  • 项目状态:
    已结题

项目摘要

Mechanistic understanding of calcium signaling in muscle contraction and regeneration will benefit from devices that can interrogate muscle dynamics at subcellular resolutions. This research project aims to lay the groundwork of optoelectronic muscle interfacing that can precisely modulate and capture the calcium dynamics in muscle. The outcome of this research will result in new muscle interfacing tools and assays, which can help better understand the mechanisms of muscle physiology, and in the long term offer a possible platform to develop therapeutics targeted to muscle recovery. The educational objectives of this proposal are aimed at training and inspiring young engineers and scientists with the multidisciplinary background required to help define the future trajectory of regenerative medicine, physical therapy, and kinesiology. The broader impacts of this project include: 1) advancing transformative muscle interfacing technologies towards the development of muscle therapeutics; 2) educating underrepresented undergraduate and graduate researchers to contribute to the nation’s workforce needs in biotechnology and healthcare, and 3) promoting muscle science and technology among local senior citizens and support groups for muscle diseases. The research objective of this proposal is to establish two high-density optoelectronic arrays to interrogate the dynamics of muscle contraction and regeneration processes at subcellular precision. To achieve this, these arrays will be built with scalable fabrication process to offer bi-directional optogenetic control and on-chip fluorescence recording of intracellular signals, respectively. In the long term, both arrays can be built along a shank structure to ultimately offer access to deep muscle tissue. The intellectual merit of the proposed work will be evidenced by the following scientific contributions: 1) subcellular optogenetic modulation of the neuromuscular junctions via a high-density light-source array; 2) subcellular imaging of the Ca2+ and voltage signals in muscle cells via a high-density photodetector array; and 3) subcellular interrogation of the muscle contraction and regeneration processes with two optoelectronic arrays, which will shed light on the development of muscle therapeutics.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
从机制上理解肌肉收缩和再生中的钙信号将受益于能够在亚细胞分辨率下询问肌肉动力学的设备。本研究项目旨在为能够精确调控和捕捉肌肉内钙动态的光电肌肉接口奠定基础。这项研究的结果将导致新的肌肉接口工具和分析,这可以帮助更好地了解肌肉生理学的机制,并从长远来看,提供了一个可能的平台,开发针对肌肉恢复的治疗方法。该提案的教育目标旨在培养和激励具有多学科背景的年轻工程师和科学家,以帮助确定再生医学、物理疗法和人体运动学的未来发展轨迹。该项目的更广泛影响包括:1)推动变革性的肌肉接口技术,以促进肌肉疗法的发展;2)教育未被充分代表的本科生和研究生研究人员,以满足国家在生物技术和医疗保健方面的劳动力需求;以及3)在当地老年人和肌肉疾病支持团体中促进肌肉科学和技术。该方案的研究目标是建立两个高密度光电阵列,以亚细胞精度询问肌肉收缩和再生过程的动力学。为了实现这一点,这些阵列将采用可扩展的制造工艺,分别提供双向光遗传控制和细胞内信号的芯片上荧光记录。从长远来看,这两个阵列都可以沿着小腿结构建造,最终提供进入深层肌肉组织的途径。这项拟议工作的智力价值将从以下科学贡献中得到证明:1)通过高密度光源阵列对神经肌肉连接进行亚细胞光遗传调制;2)通过高密度光电探测器阵列对肌肉细胞中的钙离子和电压信号进行亚细胞成像;以及3)使用两个光电阵列对肌肉收缩和再生过程进行亚细胞询问,这将有助于肌肉疗法的发展。该奖项反映了NSF的法定使命,并通过使用基金会的智力优势和更广泛的影响审查标准进行评估,被认为值得支持。

项目成果

期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Brushing-Assisted Two-Color Quantum-Dot Micro-LED Array Towards Bi-Directional Optogenetics
刷涂辅助双色量子点 Micro-LED 阵列实现双向光遗传学
  • DOI:
    10.1109/led.2021.3108554
  • 发表时间:
    2021
  • 期刊:
  • 影响因子:
    4.9
  • 作者:
    Mao, Dacheng;Xiong, Zheshun;Donnelly, Matthew;Xu, Guangyu
  • 通讯作者:
    Xu, Guangyu
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Guangyu Xu其他文献

Bibliometric Screening of Helicobacter Pylori Pathogenic Genes Through Pathway Enrichment and Operon Analysis
通过通路富集和操纵子分析对幽门螺杆菌致病基因进行文献计量筛选
  • DOI:
    10.7754/clin.lab.2016.160319
  • 发表时间:
    2016
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Guangyu Xu;Yan Xia;Ying Tang;Huansheng Cao;Qin Ma;Ying Xu;Nan Zhang;Hong Xu
  • 通讯作者:
    Hong Xu
Spray coating of carbon nanoparticles as an effective and scalable method to enhance the performance of stainless steel anode in microbial electrochemical systems
  • DOI:
    https://doi.org/10.1016/j.electacta.2023.143460
  • 发表时间:
    2024
  • 期刊:
  • 影响因子:
  • 作者:
    Hong Zhang;Jinpeng Yu;Chen Chen;Chengyong Shu;Guangyu Xu;Jie Ren;Kai Cui;Wenfang Cai;Yunhai Wang;Kun Guo
  • 通讯作者:
    Kun Guo
Boundedness and asymptotically stability to chemotaxis system with competitive kinetics and nonlocal terms
  • DOI:
  • 发表时间:
    2020-07
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Guangyu Xu
  • 通讯作者:
    Guangyu Xu
Modulating anti-dark vector bisolitons
  • DOI:
    10.1016/j.ijleo.2023.170815
  • 发表时间:
    2023-06-01
  • 期刊:
  • 影响因子:
  • 作者:
    Guangyu Xu;Keyun Zhang;Meisong Liao;Yongzheng Fang;Yan Zhou
  • 通讯作者:
    Yan Zhou
Emergence of lager densities in chemotaxis system with logistic growth and indirect signal production.
  • DOI:
  • 发表时间:
    2020-12
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Guangyu Xu
  • 通讯作者:
    Guangyu Xu

Guangyu Xu的其他文献

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{{ truncateString('Guangyu Xu', 18)}}的其他基金

Collaborative Research: The influence of mesoscale eddies on deep-sea dynamics and implications for larval connectivity along mid-ocean ridges
合作研究:中尺度涡流对深海动力学的影响以及对洋中脊幼虫连通性的影响
  • 批准号:
    2318964
  • 财政年份:
    2023
  • 资助金额:
    $ 36万
  • 项目类别:
    Standard Grant
Collaborative Research: From Magma to Vents: Monitoring Hydrothermal Fluid Temperature and Upflow-zone Permeability in Relation to Magma Movement at Axial Seamount
合作研究:从岩浆到喷口:监测热液温度和上流区渗透率与轴向海山岩浆运动的关系
  • 批准号:
    2141963
  • 财政年份:
    2022
  • 资助金额:
    $ 36万
  • 项目类别:
    Continuing Grant
CAREER: Scalable, high-precision optoelectronic lab-on-a-chip towards next-generation precision therapeutics
职业:可扩展、高精度光电芯片实验室,致力于下一代精准治疗
  • 批准号:
    2046031
  • 财政年份:
    2021
  • 资助金额:
    $ 36万
  • 项目类别:
    Continuing Grant
Collaborative Research: Hydrothermal Estuaries: What Sets the Hydrothermal Flux of Fe and Mn to the Oceans?
合作研究:热液河口:是什么决定了铁和锰进入海洋的热液通量?
  • 批准号:
    1851199
  • 财政年份:
    2019
  • 资助金额:
    $ 36万
  • 项目类别:
    Standard Grant
NCS-FO: Collaborative Research: Optoelectronic Tools for Closed-Loop Neuron Ensemble Recording and Control during Complex Behaviors
NCS-FO:协作研究:复杂行为期间闭环神经元集成记录和控制的光电工具
  • 批准号:
    1835268
  • 财政年份:
    2018
  • 资助金额:
    $ 36万
  • 项目类别:
    Standard Grant
Collaborative Research: Heat flow mapping and quantification at ASHES hydrothermal vent field using an observatory imaging sonar
合作研究:使用天文台成像声纳对 ASHES 热液喷口场进行热流测绘和量化
  • 批准号:
    1834813
  • 财政年份:
    2018
  • 资助金额:
    $ 36万
  • 项目类别:
    Continuing Grant
Collaborative Research: Heat flow mapping and quantification at ASHES hydrothermal vent field using an observatory imaging sonar
合作研究:使用天文台成像声纳对 ASHES 热液喷口场进行热流测绘和量化
  • 批准号:
    1736920
  • 财政年份:
    2017
  • 资助金额:
    $ 36万
  • 项目类别:
    Continuing Grant

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CMD 相关 α-肌营养不良聚糖糖基化酶遗传变异的大规模并行功能询问
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