CAREER: Scalable, high-precision optoelectronic lab-on-a-chip towards next-generation precision therapeutics

职业：可扩展、高精度光电芯片实验室，致力于下一代精准治疗

• 批准号: 2046031
• 负责人: Guangyu Xu
• 金额: $ 50万
• 依托单位: University of Massachusetts Amherst
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-04-01 至 2026-03-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2046031&HistoricalAwards=false
• 关键词: CAREER; Scalable; precision; optoelectronic; lab

Precision therapeutics will benefit from biomedical devices that can acquire cellular/molecular information specific to targeted patient groups. This research project aims to establish scalable, high-precision lab-on-a-chip technologies that will ultimately lead to enabling tools in next-generation precision therapeutics. The outcome of this research will result in new engineering tools and disease related assays that will likely make an impact in pharmatheutical screening, immunotherapeutic development, and point-of-care systems. The educational objectives of this proposal are aimed at training and inspiring young engineers and scientists who are equipped with the multidisciplinary background required to help define the future trajectory of personalized medicine, neuroengineering, and point-of-care systems. The broader impacts of this project include: 1) advancing transformative device technologies for scalable, high-precision lab-on-a-chip systems and providing powerful tools for pharmatheutics and point-of-care testing; 2) educating underrepresented undergraduate and graduate researchers to contribute to the nation’s workforce needs in bioengineering and healthcare; 3) contributing to the K-12 science, technology, engineering, and mathematics education by weekend seminars and mentoring student-teacher pairs from local middle schools; and 4) promoting biological sciences and biotechnology among local senior citizens and support groups for neurological diseases and immunological disorders. The research objective of this proposal is to establish two high-precision optoelectronic lab-on-a-chips built with scalable fabrication process, aiming to offer high-content analysis at cellular and molecular levels towards next-generation precision therapeutics. Both lab-on-a-chips will be built in a highly scalable array form, with their precision being evaluated in cultured neuronal network and blood samples, respectively. The proposed work will open ample research opportunities in drug screening for neurological diseases and point-of-care testing for patients with acute immune symptoms. The intellectual merit of the proposed work will be evidenced by three major contributions: 1) a scalable, high-precision optoelectronic lab-on-a-chip capable of precise optogenetic control, electrical recording, and electrical field stimulation of neural activity; 2) a scalable, high-precision optoelectronic lab-on-a-chip capable of multiplexed on-chip fluorescence detection of biomarkers that are related to immune responses; and 3) drug-screening and immune response monitoring assays using these two lab-on-a-chips, which will shed light on therapeutics development targeted to neurological and autoimmune diseases.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

精确治疗将受益于生物医学设备，可以获取特定于目标患者群体的细胞/分子信息。该研究项目旨在建立可扩展的，高精度的芯片实验室技术，最终将导致下一代精确治疗的工具。这项研究的结果将产生新的工程工具和疾病相关的测定，可能会对药物筛选，免疫学开发和即时护理系统产生影响。该提案的教育目标旨在培养和激励年轻的工程师和科学家，他们具备帮助定义个性化医疗，神经工程和护理点系统未来发展轨迹所需的多学科背景。该项目的更广泛影响包括：1）推进可扩展的高精度芯片实验室系统的变革性设备技术，并为制药和即时检测提供强大的工具; 2）教育代表性不足的本科生和研究生研究人员，为国家在生物工程和医疗保健方面的劳动力需求做出贡献; 3）通过周末研讨会和指导当地中学的师生对，为K-12科学，技术，工程和数学教育做出贡献;及四）向本地长者及神经系统疾病及免疫系统疾病支援团体推广生物科学及生物科技。 该提案的研究目标是建立两个高精度的光电芯片实验室，采用可扩展的制造工艺，旨在为下一代精确治疗提供细胞和分子水平的高内容分析。这两个芯片实验室将以高度可扩展的阵列形式构建，分别在培养的神经元网络和血液样本中评估其精度。拟议的工作将为神经系统疾病的药物筛选和急性免疫症状患者的即时检测提供充足的研究机会。所提出的工作的智力价值将通过三个主要贡献来证明：1）可扩展的、高精度的光电芯片实验室，其能够精确地光遗传学控制、电记录和神经活动的电场刺激; 2）可扩展的、高精度的光电芯片实验室，其能够对与免疫应答相关的生物标志物进行多路复用的芯片上荧光检测;以及3）使用这两个芯片实验室的药物筛选和免疫反应监测分析，这将揭示针对神经和自身免疫性疾病的治疗方法的发展。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Spectrally filtered photodiode pairs for on-chip ratiometric aptasensing of cytokine dynamics

• DOI: 10.1016/j.snb.2021.130330
• 发表时间: 2021-10
• 期刊: Sensors and Actuators B-chemical
• 影响因子: 8.4
• 作者: Z. Xiong;Kewei Ren;M. Donnelly;M. You;Guangyu Xu

Brushing-Assisted Two-Color Quantum-Dot Micro-LED Array Towards Bi-Directional Optogenetics

刷涂辅助双色量子点 Micro-LED 阵列实现双向光遗传学

• DOI: 10.1109/led.2021.3108554
• 发表时间: 2021
• 期刊: IEEE Electron Device Letters
• 影响因子: 4.9
• 作者: Mao, Dacheng;Xiong, Zheshun;Donnelly, Matthew;Xu, Guangyu
• 通讯作者: Xu, Guangyu

Close-Packed PEDOT:PSS-Coated Graphene Microelectrodes for High-Resolution Interrogation of Neural Activity

密堆积 PEDOT:PSS 涂层石墨烯微电极用于神经活动的高分辨率检测

• DOI: 10.1109/ted.2021.3074353
• 发表时间: 2021
• 期刊: IEEE Transactions on Electron Devices
• 影响因子: 3.1
• 作者: Sun, Feng;Xiong, Zheshun;Park, Jinyoung;Xu, Guangyu
• 通讯作者: Xu, Guangyu

A Silicon Neural Probe Monolithically Integrated with 20 µm-Pitched Dual-Color Micro-Led Arrays

与 20 µm 间距双色 Micro-Led 阵列单片集成的硅神经探针

• DOI: 10.1109/mems58180.2024.10439308
• 发表时间: 2024
• 期刊: 2024 IEEE 37th International Conference on Micro Electro Mechanical Systems (MEMS
• 作者: Mao, Dacheng;Sun, Feng;Driscoll, Bradley;Li, Zhihao;Xu, Guangyu
• 通讯作者: Xu, Guangyu