Elucidating Fundamental Factors Driving Self-assembly with Guided Interactions in Multicomponent Enzyme Systems Using Model Nanostructured Platforms

使用模型纳米结构平台阐明多组分酶系统中通过引导相互作用驱动自组装的基本因素

• 批准号: 2108448
• 负责人: Cindy Berrie
• 金额: $ 50.85万
• 依托单位: University of Kansas Center for Research Inc
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-07-01 至 2025-06-30
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2108448&HistoricalAwards=false
• 关键词: Elucidating; Fundamental; Factors; Driving; Self

With the support of the Macromolecular, Supramolecular and Nanochemistry program in the Division of Chemistry and the Established Program to Stimulate Competitive Research (EPSCoR), Professors Cindy L. Berrie and Candan Tamerler at the University of Kansas are investigating factors that govern the assembly and organization of biomolecules at interfaces. Affinity peptide tags will be used to selectively direct the self-assembly of biomolecules, including enzymes, onto material surfaces to create multicomponent bioactive materials organized at the nanoscale. The metal nanostructure platforms being developed are designed to enable an understanding of the role of material specificity, curvature, spacing, and size on the spatially organized self-assembly of biomolecules. The project will allow biohybrid materials to mimic the exquisite functionality nature has evolved for complex tasks, which will enable enhanced biosensing, biocatalysis and biofuel applications as an alternative energy source. In the course of conducting the project, graduate and undergraduate students will be trained in the growing convergence of nanoscience, biomolecules and biomaterials. In addition, the research team will carry out outreach and services to the community at the University of Kansas and local middle and elementary schools through participation in the Engineering EXPO and the Carnival of Chemistry events and the development of the “Science Night” program to engage students in science at an early stage. Public demonstrations on nanolithography and imaging will be conducted with the involvement of the students working on the project. The project focuses on exploring the fundamental factors responsible for peptide guided self-assembly of multi-enzyme systems using model nanostructured platforms to harness their coordinated activity. Nature exquisitely organizes cascades of enzymes to work in tandem; however, attempts to artificially assemble such complex systems are hampered by the complexity and lack of information about the factors governing functional assembly. Emerging applications from biocatalysis to biosensing, to energy harvesting and biofuels would likely benefit from assembly of coupled enzymes with cascade-like activity, and therefore elucidating the factors controlling the assembly of such complex systems would have wide ranging applications. Specifically, the assembly of peptide tags, peptide-labeled enzymes, and the co-assembly of coupled enzyme pairs will be investigated using optical and atomic force microscopy as well as bioactivity assays to determine the distribution, conformation, and orientation of assembled biomolecules and how these are affected by the metal nanostructure composition, spacing, size, and curvature. The scientific broader impacts of the work include the development of design principles for biohybrid materials for applications in biosensing and biocatalysis. There are also important elements of workforce development in the area of nanobiomaterials and of outreach the community.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

在化学学部的大分子、超分子和纳米化学项目以及促进竞争研究的既定项目（EPSCoR）的支持下，堪萨斯大学的Cindy L. Berrie教授和Candan Tamerler教授正在研究控制界面上生物分子组装和组织的因素。亲和肽标签将用于选择性地指导生物分子（包括酶）在材料表面的自组装，以创建纳米级组织的多组分生物活性材料。正在开发的金属纳米结构平台旨在使人们能够理解材料特异性、曲率、间距和尺寸在生物分子的空间组织自组装中的作用。该项目将允许生物混合材料模仿自然界为复杂任务而进化出的精致功能，这将增强生物传感、生物催化和生物燃料作为替代能源的应用。在进行该项目的过程中，研究生和本科生将接受纳米科学、生物分子和生物材料日益融合的培训。此外，研究团队将通过参与工程博览会和化学嘉年华活动，以及制定“科学之夜”计划，在堪萨斯大学和当地中小学开展社区推广和服务，让学生在早期阶段参与科学。在参与该项目的学生的参与下，将进行纳米光刻和成像的公开演示。该项目的重点是探索多肽引导多酶系统自组装的基本因素，使用模型纳米结构平台来利用它们的协调活性。大自然巧妙地组织酶级联协同工作；然而，人工组装这种复杂系统的尝试受到复杂性和缺乏有关控制功能组装因素的信息的阻碍。从生物催化到生物传感，到能量收集和生物燃料的新兴应用可能受益于具有级联样活性的偶联酶的组装，因此阐明控制这种复杂系统组装的因素将具有广泛的应用。具体来说，肽标签的组装、肽标记酶的组装以及偶联酶对的共组装将使用光学和原子力显微镜以及生物活性测定来确定组装生物分子的分布、构象和方向，以及这些如何受到金属纳米结构组成、间距、大小和曲率的影响。这项工作的更广泛的科学影响包括开发用于生物传感和生物催化的生物混合材料的设计原则。在纳米生物材料和社区外展领域也有劳动力发展的重要因素。该奖项反映了美国国家科学基金会的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。