Next-Generation Optical Contrasts for Characterization of Biological Tissue, Hard Matter, and Cultural Heritage Objects

用于表征生物组织、硬物质和文化遗产物体的下一代光学对比

• 批准号: 2108623
• 负责人: Martin Fischer
• 金额: $ 51.04万
• 依托单位: Duke University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2108623&HistoricalAwards=false
• 关键词: Next; Generation; Optical; Contrasts; Characterization

With support from the Chemical Measurement and Imaging (CMI) Program in the Division of Chemistry, Drs. Martin Fischer and Warren Warren at Duke University will develop optical microscopy techniques to investigate the three-dimensional structure and function of a wide range of materials, including biological tissue and pigments used in historical artwork. Traditionally, these complex materials pose severe challenges for optical imaging and often the samples need to be cut open to investigate layers below the surface. Newly developed imaging methods allow for three-dimensional imaging without sample destruction and the investigators are developing techniques to extend the range of chemical contrast, the depth of imaging, and the ability to interpret the results. This development has the potential to detect microscopic changes in skin and artwork during the onset of deterioration, which could aid in early diagnosis and better choice of treatment options. The methods developed by this program benefit academic research and could lead to a device of great interest to biomedical researchers and conservators alike.Multiple ultrafast laser pulses can interact with matter via a wide range of nonlinear processes. The investigators have pioneered an imaging method, pump-probe microscopy, that turns such interactions into detectable contrast for imaging with low average power at high speeds and they have demonstrated applications in biological tissue, historical artworks, and engineered materials. The signals acquired with this microscopy method depend on many parameters, such as the laser pulse wavelengths, inter-pulse delay, relative polarization, and detection direction. The range of parameters presents a challenge due to the complexity of the response, but also an opportunity for recording images using multiple parameters to provide different contrast types and a diverse and comprehensive view of the sample. The investigators are developing a pump-probe microscopy platform that can efficiently, safely, and rapidly acquire multi-modal, multi-parameter data sets that provide images with molecular specificity for biology and cultural heritage. Specifically, the investigators and their team will pursue the following research goals: (i) systematically investigate and model the dependence on imaging and sample parameters (i.e., wavelength, polarization, scattering angle) to separate the complex set of nonlinear optical interactions and decompose the images into fundamental components; and (ii) develop a coherent femtosecond pulse shaping and detection approach for efficient multi-modal and multi-parameter acquisition. The research seeks to develop a microscope that will be able to non-invasively extract comprehensive molecular and structural information in biological tissue and historical artwork.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

在化学系化学测量和成像（CMI）项目的支持下，杜克大学的Martin Fischer和Warren博士将开发光学显微镜技术，以研究各种材料的三维结构和功能，包括生物组织和历史艺术品中使用的颜料。传统上，这些复杂的材料对光学成像提出了严峻的挑战，通常需要将样品切开以研究表面以下的层。新开发的成像方法允许在不破坏样品的情况下进行三维成像，研究人员正在开发技术以扩展化学对比度的范围、成像的深度和解释结果的能力。这一发展有可能在恶化开始期间检测皮肤和艺术品的微观变化，这可能有助于早期诊断和更好地选择治疗方案。该计划开发的方法有利于学术研究，并可能导致生物医学研究人员和保护者非常感兴趣的设备。多个超快激光脉冲可以通过广泛的非线性过程与物质相互作用。研究人员开创了一种成像方法，即泵浦-探测显微镜，将这种相互作用转化为可检测的对比度，以便在高速下以低平均功率成像，并且他们已经展示了在生物组织，历史艺术品和工程材料中的应用。用这种显微镜方法获得的信号取决于许多参数，例如激光脉冲波长、脉冲间延迟、相对偏振和检测方向。由于响应的复杂性，参数的范围提出了挑战，但也提供了使用多个参数记录图像以提供不同对比度类型和样品的多样化和全面视图的机会。研究人员正在开发一种泵探测显微镜平台，可以有效，安全，快速地获取多模态，多参数数据集，为生物和文化遗产提供具有分子特异性的图像。具体而言，研究人员及其团队将追求以下研究目标：（i）系统地研究和建模对成像和样本参数的依赖性（即，波长，偏振，散射角），以分离复杂的一组非线性光学相互作用，并分解成基本组件的图像;和（ii）开发一个相干飞秒脉冲整形和检测方法，有效的多模式和多参数采集。该研究旨在开发一种能够非侵入性地从生物组织和历史艺术品中提取全面分子和结构信息的显微镜。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Contrast mechanisms in pump-probe microscopy of melanin

黑色素泵探针显微镜的对比机制

• DOI: 10.1364/oe.469506
• 发表时间: 2022
• 期刊: Optics Express
• 影响因子: 3.8
• 作者: Grass, David;Beasley, Georgia M.;Fischer, Martin C.;Selim, M. Angelica;Zhou, Yue;Warren, Warren S.
• 通讯作者: Warren, Warren S.