Collaborative Research: EAGER: Developing tools to assess the evolutionary implications of partial clonality in alpine snow algae

合作研究：EAGER：开发工具来评估高山雪藻部分克隆性的进化影响

• 批准号: 2113745
• 负责人: Charles Amsler
• 金额: $ 12.94万
• 依托单位: University of Alabama at Birmingham
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-04-01 至 2024-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2113745&HistoricalAwards=false
• 关键词: Collaborative; Research; EAGER; Developing; tools

Many globally important processes like carbon cycling and human disease dynamics are a result of microscopic eukaryotes. Yet our understanding of evolutionary processes across the entire tree of life is limited due to the inherent challenges of studying microscopic taxa. Tools developed for macroscopic, and often obligately sexually reproducing, species are often not tractable in microbes where generation times are short, population sizes large, and extracting DNA from individual cells difficult. Further, population-level ecology and evolutionary biology research have focused on species that can be grown in the lab; yet, only ~1% of microbes can be cultured. This proposal focuses on the snow algae, a group of closely related, single-celled algae that turn seasonal snow packs pink or red. The aim is to develop novel single-cell population genetic protocols to study these threatened organisms and sentinels of environmental change. Thousands of cells from inter- and intra-annual Chlainomonas-dominated blooms in the Cascade Range of the United States will be genotyped. A goal is to understand how environmental factors (e.g., nitrogen) influence snow algal reproduction. The project will support two graduate students. The three PIs will integrate data into classroom-based and field-based curriculum for university-level students and share data with the public through social media and peer-reviewed publications.This project uses snow algae to develop new methods for studying the population genetics of microbial eukaryotes from natural populations. The work will provide novel and critical techniques for studying reproductive modes that will be broadly applicable across microbial systems. Two novel elements are proposed: (1) combining single-cell isolation methods with state-of-the-art multilocus genotyping, and (2) using a novel microbial eukaryotic system (snow algae) with features making the method development tractable. Reproductive mode data will be generated and combined with measurements of (i) the ploidy of cells and (ii) environmental conditions to ascertain whether limited nitrogen initiates sexual reproduction. The approach is radically different compared to traditional microalgal population genetics that rely on lab-based cultures, raising questions of how representative those data are in nature. This proposal fits the EAGER funding mechanism as the single-cell population genetic methods are high risk-high payoff and should be readily transferable to other systems.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

许多全球重要的过程，如碳循环和人类疾病动力学是微观真核生物的结果。然而，由于研究微观类群的固有挑战，我们对整个生命之树的进化过程的理解是有限的。为宏观而开发的工具，通常是强制性的有性繁殖，物种在微生物中往往不容易处理，因为微生物的世代时间很短，种群规模很大，从单个细胞中提取DNA很困难。此外，种群水平的生态学和进化生物学研究集中在可以在实验室中生长的物种上;然而，只有1%的微生物可以培养。这项提案的重点是雪藻，一组密切相关的单细胞藻类，使季节性积雪变成粉红色或红色。其目的是开发新型单细胞群体遗传方案来研究这些受威胁的生物和环境变化的哨兵。将对美国喀斯喀特山脉每年内和每年间以衣原体为主的水华中的数千个细胞进行基因分型。一个目标是了解环境因素（例如，氮）影响雪藻繁殖。该项目将资助两名研究生。这三个PI将把数据整合到大学生的课堂和实地课程中，并通过社交媒体和同行评议的出版物与公众分享数据。该项目使用雪藻开发研究自然种群微生物真核生物种群遗传学的新方法。这项工作将为研究繁殖模式提供新的关键技术，这些技术将广泛适用于整个微生物系统。提出了两个新的元素：（1）结合单细胞分离方法与国家的最先进的多位点基因分型，和（2）使用一种新的微生物真核系统（雪藻）的功能，使方法的发展听话。将生成生殖模式数据，并与（i）细胞倍性和（ii）环境条件的测量结果相结合，以确定有限的氮是否会启动有性生殖。这种方法与传统的依赖于实验室培养的微藻种群遗传学相比有着根本的不同，这就提出了这些数据在自然界中的代表性问题。这项提议符合EAGER的资助机制，因为单细胞群体遗传学方法是高风险高回报的，应该很容易转移到其他系统。这项奖励反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。