MRI: Acquisition of a MALDI-TOF Mass Spectrometer to Advance Research and Undergraduate Research Training

MRI：购买 MALDI-TOF 质谱仪以推进研究和本科生研究培训

• 批准号: 2117141
• 负责人: George Geier
• 金额: $ 29.9万
• 依托单位: Colgate University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-08-01 至 2024-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2117141&HistoricalAwards=false
• 关键词: MRI; Acquisition; MALDI; TOF; Mass

This award is supported by the Major Research Instrumentation and the Chemistry Research Instrumentation programs. Colgate University is acquiring a matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometer to support Professor George Geier and colleagues Ernest Nolen, Jacob Goldberg and Jennifer Peeler. In general, mass spectrometry (MS) is one of the key analytical methods used to identify and characterize small quantities of chemical species in complex samples. MALDI TOF combines gentle ionization (ideal for producing intact ions of peptides, proteins, nucleic acids, carbohydrates, synthetic polymers, and other similarly sized species) with a detection mode that offers an excellent balance between sensitivity and accuracy across a wide mass range. This highly sensitive technique allows identification and determination of the structure of molecules in a complex mixture. The acquisition strengthens the research infrastructure at the university and regional area. The instrument broadens participation by involving diverse students with this modern analytical technique. It is also used by collaborators at Hamilton College.The award of the MALDI-TOF mass spectrometer is aimed at enhancing research and education at all levels. It is especially useful to determine masses of synthetic glycopeptide mimics and their synthetic intermediates and for the development of chemical probes tethered to short peptides to detect zinc(II) ions in the nervous system. The instrumentation is also used for monitoring the progress of peptide synthesis reactions, as well as for identifying products obtained via high-performance liquid chromatography (HPLC)-based separations emanating from synthetic ventures. The instrument also serves researchers assessing expression of human selenoproteins via genetic code expansion, and carrying out assays of selenoprotein enzymatic activity.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该奖项由主要研究仪器和化学研究仪器计划支持。高露洁大学正在购买一台基质辅助激光解吸/电离飞行时间（MALDI-TOF）质谱仪，以支持乔治盖尔教授及其同事欧内斯特·诺伦、雅各布·戈德堡和詹妮弗·皮勒。一般来说，质谱（MS）是用于识别和表征复杂样品中少量化学物质的关键分析方法之一。MALDI TOF结合了温和的离子化（非常适合产生肽、蛋白质、核酸、碳水化合物、合成聚合物和其他类似大小的物质的完整离子）和检测模式，在广泛的质量范围内提供灵敏度和准确度之间的出色平衡。这种高灵敏度的技术可以识别和确定复杂混合物中分子的结构。此次收购加强了大学和地区的研究基础设施。该仪器通过让不同的学生参与这种现代分析技术来扩大参与。汉密尔顿学院的合作者也在使用它。授予MALDI-TOF质谱仪的目的是加强各级的研究和教育。它是特别有用的，以确定质量的合成糖肽模拟物和它们的合成中间体，并为发展化学探针栓到短肽检测锌（II）离子在神经系统中。 该仪器还用于监测肽合成反应的进展，以及用于识别通过基于高效液相色谱（HPLC）的分离从合成企业获得的产品。该仪器还可用于研究人员通过遗传密码扩展评估人类硒蛋白的表达，并进行硒蛋白酶活性测定。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Ultra-rapid Electrophilic Cysteine Arylation.

• DOI: 10.1021/jacs.3c10334
• 发表时间: 2023-10
• 期刊: Journal of the American Chemical Society
• 影响因子: 15
• 作者: Bradley M Lipka;Daniel S Honeycutt;Gregory M Bassett;Taylor N Kowal;Max Adamczyk;Zachary C Cartnick;V. Betti;Jacob M. Goldberg;Fang Wang