MRI: Acquisition of a MALDI Tandem Mass Spectrometer (MALDI MS) as an analytical tool to support multidisciplinary research at University of South Carolina

该奖项由主要研究仪器和化学研究仪器项目支持。来自南卡罗来纳大学哥伦比亚分校的王茜教授及其同事Linda Shimizu， Johannes Stratmann, Michael Walla， Chang Liu正在获得一个配备光电二极管阵列的基质辅助激光解吸/电离飞行时间质谱仪（MALDI-TOF-MS）。一般来说，质谱（MS）是用于鉴定和表征复杂样品中少量化学物质的关键分析方法之一。MALDI TOF结合了温和的电离（理想的生产肽，蛋白质，核酸，碳水化合物，合成聚合物和其他类似大小的物种的完整离子）的检测模式，提供了灵敏度和准确性之间的良好平衡，在广泛的样品。此次收购加强了该大学和该地区的研究基础设施。该工具通过为不同的学生群体提供实际接触机会来扩大参与。该工具还用于外联活动。该奖项旨在加强各级的研究和教育。它尤其影响了分子结构、取向和动力学方面的研究。研究人员使用MALDI-TOF-MS来研究蛋白质和蛋白质基纳米颗粒的生物偶联产物分析、超分子组装、纳米反应器和共轭聚合物、细胞内藻类毒素成像、蛋白质和DNA表征、金属-有机杂化物的电子和光物理、有机配体分析、硼酸修饰肽分析、功能聚合物的分子量分析。微生物多肽和小蛋白的表征、植物与病原体相互作用的研究、用于临床诊断的生物传感器和生物标志物、细胞和组织靶向药物递送、蛋白质相互作用研究、血管结构和组织支架成像、药代动力学和药效学研究、血管结构成像、血管结构成像、分析可生物降解聚合物、用于组织工程应用的树突状生物材料、有机金属合成配体分析，不溶性有机分子分析。该奖项反映了美国国家科学基金会的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

This award is supported by the Major Research Instrumentation and the Chemistry Research Instrumentation Programs. Professor Qian Wang and colleagues Linda Shimizu, Johannes Stratmann, Michael Walla, Chang Liu from the University of South Carolina - Columbia, are acquiring a matrix-assisted laser desorption / ionization time-of-flight mass spectrometer (MALDI-TOF-MS) equipped with a photodiode array. In general, mass spectrometry (MS) is one of the key analytical methods used to identify and characterize small quantities of chemical species in complex samples. MALDI TOF combines gentle ionization (ideal for producing intact ions of peptides, proteins, nucleic acids, carbohydrates, synthetic polymers, and other similarly sized species) with a detection mode that offers an excellent balance between sensitivity and accuracy across a wide range of samples. The acquisition strengthens the research infrastructure at the university and within the regional. The instrument broadens participation by giving hands-on access to a diverse student population. The instrument is also used in outreach activities. The award is aimed at enhancing research and education at all levels. It especially impacts studies correlating molecular structure, orientation and dynamics. Researchers use the MALDI-TOF-MS to study analysis of bioconjugation products of proteins and protein-based nanoparticles, supramolecular assemblies, nanoreactors and conjugated polymers, imaging of intracellular algal toxins, protein & DNA characterization, electronics and photophysics of metal-organic hybrids, organic ligand analysis , analysis of boronic acid modified peptides, molecular weight analysis of functional polymers, characterization of peptides and small proteins from microbes, study of plant-pathogen interactions, biosensors and biomarkers for clinical diagnosis, cell and tissue targeted drug delivery, study the protein interactions, imaging of vascular structures and tissue scaffolds, pharmacokinetics and pharmacodynamics studies, imaging of vascular structures, imaging of vascular structures, analysis biodegradable polymers, dendrimer based biomaterials for tissue engineering applications, analysis of ligands for organometallic synthesis, analysis of insoluble organic molecules.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.