MRI: Acquisition of a MALDI Tandem Mass Spectrometer (MALDI MS) as an analytical tool to support multidisciplinary research at University of South Carolina

该奖项由主要研究仪器项目和化学研究仪器项目支持。南卡罗来纳大学哥伦比亚分校的王倩教授及其同事Linda Shimizu、Johannes Stratmann、Michael Walla、Chang Liu正在获得一台配备光电二极管阵列的基质辅助激光解吸/电离飞行时间质谱仪(MALDI-TOF-MS)。通常，质谱仪是用于鉴定和表征复杂样品中少量化学物种的关键分析方法之一。MALDI TOF结合了温和的电离(非常适合生产完整的多肽、蛋白质、核酸、碳水化合物、合成聚合物和其他类似大小物种的离子)和检测模式，在各种样品的灵敏度和准确性之间提供了极好的平衡。此次收购加强了该大学和该地区的研究基础设施。该工具通过向不同的学生群体提供亲身实践机会来扩大参与度。该工具还用于外联活动。该奖项旨在加强各级的研究和教育。它尤其影响了与分子结构、取向和动力学相关的研究。研究人员使用MALDI-TOF-MS研究蛋白质和基于蛋白质的纳米颗粒、超分子组件、纳米反应器和共轭聚合物的生物结合产物的分析，细胞内藻毒素的成像，蛋白质和AMP；DNA表征，金属-有机杂化化合物的电子学和光物理，有机配体分析，硼酸修饰的多肽的分析，功能聚合物的分子量分析，来自微生物的多肽和小蛋白的特性，植物-病原体相互作用的研究，用于临床诊断的生物传感器和生物标志物，细胞和组织靶向药物输送，研究蛋白质相互作用，血管结构和组织支架的成像，药代动力学和药效学研究，血管结构的成像，血管结构的成像，分析可生物降解的聚合物，用于组织工程的树枝状大分子生物材料，金属有机合成的配基分析，不溶性有机分子的分析。这一奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。

This award is supported by the Major Research Instrumentation and the Chemistry Research Instrumentation Programs. Professor Qian Wang and colleagues Linda Shimizu, Johannes Stratmann, Michael Walla, Chang Liu from the University of South Carolina - Columbia, are acquiring a matrix-assisted laser desorption / ionization time-of-flight mass spectrometer (MALDI-TOF-MS) equipped with a photodiode array. In general, mass spectrometry (MS) is one of the key analytical methods used to identify and characterize small quantities of chemical species in complex samples. MALDI TOF combines gentle ionization (ideal for producing intact ions of peptides, proteins, nucleic acids, carbohydrates, synthetic polymers, and other similarly sized species) with a detection mode that offers an excellent balance between sensitivity and accuracy across a wide range of samples. The acquisition strengthens the research infrastructure at the university and within the regional. The instrument broadens participation by giving hands-on access to a diverse student population. The instrument is also used in outreach activities. The award is aimed at enhancing research and education at all levels. It especially impacts studies correlating molecular structure, orientation and dynamics. Researchers use the MALDI-TOF-MS to study analysis of bioconjugation products of proteins and protein-based nanoparticles, supramolecular assemblies, nanoreactors and conjugated polymers, imaging of intracellular algal toxins, protein & DNA characterization, electronics and photophysics of metal-organic hybrids, organic ligand analysis , analysis of boronic acid modified peptides, molecular weight analysis of functional polymers, characterization of peptides and small proteins from microbes, study of plant-pathogen interactions, biosensors and biomarkers for clinical diagnosis, cell and tissue targeted drug delivery, study the protein interactions, imaging of vascular structures and tissue scaffolds, pharmacokinetics and pharmacodynamics studies, imaging of vascular structures, imaging of vascular structures, analysis biodegradable polymers, dendrimer based biomaterials for tissue engineering applications, analysis of ligands for organometallic synthesis, analysis of insoluble organic molecules.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.