MRI: Acquisition of a Western Blot Detection Equipment for core facility of a community college

纽约城市大学(CUNY)、昆斯伯勒社区学院(QCC)校区(生物科学与地质系)正在被授予购买Li-COR奥德赛成像系统的奖项。这一多任务成像系统允许对定量Western blots(WB)、细胞内Western分析、病毒滴定分析和核酸凝胶成像进行可视化--仅举几个例子。昆士兰州立大学是纽约市立大学内的七所社区学院之一，服务于不同群体的第一代大学生，并招收了许多原本无法接受高等教育的学生。84%的QCC学生是非洲裔、亚裔或拉丁裔，超过50%的学生来自收入低于25,000美元的家庭。生物科学和地质学系每学期都有超过三分之一的教职员工在研究技术和伦理学方面指导学生。当我们的学生在STEM领域开始他们的职业生涯时，我们的教职员工对他们的成功投入了极大的精力。收购这一系统将补充校园的本科生研究核心设施，其中部分包括一台实时聚合酶链式反应设备，一台FACS机器，和一台共焦显微镜。核心设施中的所有仪器设备都允许教师向学生介绍尖端的实验方法，并帮助他们为进入STEM劳动力做好准备。新收购的Li-COR Odysey仪器也将是昆士兰大学生物技术项目和新成立的生物助理项目教育培训的组成部分，因此影响到更多的学生。利科奥德赛是昆士兰大学及其姊妹校区皇后学院持续研究和教学课程的重要组成部分。它的收购将支持各种不同的项目。这些研究包括：(A)STAT3在细胞间相互作用中的分子调控，(B)细胞内寄生虫对宿主氨基酸转运体的调控，(C)卷柏属植物中保守的根发育基因的检测，(D)在大肠杆菌中内在地展开的多肽底物对DNAK的抑制，(E)用单壁纳米管治疗后癌细胞基因的表达的研究，(F)对肿瘤行为的分子调控，(G)研究与肥胖及其相关的共病有关的新蛋白质，(H)CDX1作为结肠癌化疗药物敏感性调节因子的作用，以及(I)与心血管健康相关的研究。使用该仪器产生的数据将由教职员工和学生通过同行评议的期刊出版物以及在区域和国家举办的专业和科学会议上传播。因此，购买这一系统将为QCC最近招聘的几名早期职业调查人员及其学生开辟新的研究领域。该奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。

An award is being made to The City University of New York (CUNY), Queensborough Community College (QCC) campus (Department of Biological Sciences & Geology), to purchase a LI-COR Odyssey Imaging System. This multitasking imaging system allows for the visualization of Quantitative Western blots (WB), In-Cell Western Assays, Viral Titration Assays, and Nucleic Acid Gel Imaging – to name a few of its applications. QCC is one of the seven community colleges within CUNY and serves a diverse population of first-generation college students and admits many students who would otherwise not have access to higher education. 84% of QCC students are African American, Asian, or Latino, and more than 50% come from families with incomes of less than $25,000. More than a third of the Biological Sciences and Geology department’s faculty members mentor students in research techniques and ethics every semester. Our faculty members are exceedingly invested in the success of our students as they embark on their careers in STEM fields. Acquisition of this system will complement the campus’s undergraduate research core facility, which in part includes a real-time PCR equipment, a FACS machine, and a confocal microscope. All instrumentation in the core facility allows faculty to introduce students to cutting-edge experimental methodologies and helps prepare them for entry into the STEM workforce. The newly acquired LI-COR Odyssey instrument will also be an integral part of the educational training in the Biotechnology Program and the newly formed Associates Program in Biology at QCC, thus impacting even more students. The LI-COR Odyssey is an essential part of the continuous research effort and teaching curriculum at QCC and its sister campus, Queens College. Its acquisition will support a variety of diverse projects. These include studies of (a) the molecular regulation of STAT3 in cell-cell interactions, (b) regulation of host amino acid transporters by an intracellular parasite, (c) examination of conserved root development genes in Selaginella (d) inhibition of DnaK by intrinsically unfolded polypeptide substrates in Escherichia coli, (e) investigation of cancer cell gene expression after treatment with single-walled nanotubes, (f) the molecular regulation of Cnidarian behaviors, (g) investigating novel proteins in obesity and associated comorbidities, (h) role of CDX1 as a regulator of colon cancer sensitivity to chemotherapeutic drugs and (i) cardiovascular health-related research. Data generated using the instrument will be disseminated by faculty and students through peer-reviewed journal publications and at both regionally and nationally held professional and scientific conferences. Procuring this system will thus open new frontiers of research for several of QCC’s most recently recruited early career investigators and their students.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.