MRI: Acquisition of a Western Blot Detection Equipment for core facility of a community college

MRI：为社区学院的核心设施购置蛋白质印迹检测设备

• 批准号: 2117255
• 负责人: Sarbani Ghoshal
• 金额: $ 7.96万
• 依托单位: CUNY Queensborough Community College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-10-01 至 2024-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2117255&HistoricalAwards=false
• 关键词: MRI; Acquisition; Western; Blot; Detection

An award is being made to The City University of New York (CUNY), Queensborough Community College (QCC) campus (Department of Biological Sciences & Geology), to purchase a LI-COR Odyssey Imaging System. This multitasking imaging system allows for the visualization of Quantitative Western blots (WB), In-Cell Western Assays, Viral Titration Assays, and Nucleic Acid Gel Imaging – to name a few of its applications. QCC is one of the seven community colleges within CUNY and serves a diverse population of first-generation college students and admits many students who would otherwise not have access to higher education. 84% of QCC students are African American, Asian, or Latino, and more than 50% come from families with incomes of less than $25,000. More than a third of the Biological Sciences and Geology department’s faculty members mentor students in research techniques and ethics every semester. Our faculty members are exceedingly invested in the success of our students as they embark on their careers in STEM fields. Acquisition of this system will complement the campus’s undergraduate research core facility, which in part includes a real-time PCR equipment, a FACS machine, and a confocal microscope. All instrumentation in the core facility allows faculty to introduce students to cutting-edge experimental methodologies and helps prepare them for entry into the STEM workforce. The newly acquired LI-COR Odyssey instrument will also be an integral part of the educational training in the Biotechnology Program and the newly formed Associates Program in Biology at QCC, thus impacting even more students. The LI-COR Odyssey is an essential part of the continuous research effort and teaching curriculum at QCC and its sister campus, Queens College. Its acquisition will support a variety of diverse projects. These include studies of (a) the molecular regulation of STAT3 in cell-cell interactions, (b) regulation of host amino acid transporters by an intracellular parasite, (c) examination of conserved root development genes in Selaginella (d) inhibition of DnaK by intrinsically unfolded polypeptide substrates in Escherichia coli, (e) investigation of cancer cell gene expression after treatment with single-walled nanotubes, (f) the molecular regulation of Cnidarian behaviors, (g) investigating novel proteins in obesity and associated comorbidities, (h) role of CDX1 as a regulator of colon cancer sensitivity to chemotherapeutic drugs and (i) cardiovascular health-related research. Data generated using the instrument will be disseminated by faculty and students through peer-reviewed journal publications and at both regionally and nationally held professional and scientific conferences. Procuring this system will thus open new frontiers of research for several of QCC’s most recently recruited early career investigators and their students.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

纽约城市大学（CUNY）昆斯伯勒社区学院（QCC）校区（生物科学与地质系）获得了一份购买LI-COR奥德赛成像系统的合同。这种多任务成像系统允许可视化定量Western blots (WB)，细胞内Western检测，病毒滴定检测和核酸凝胶成像-仅举其应用的几个例子。QCC是纽约市立大学七所社区学院之一，服务于多元化的第一代大学生，并招收了许多否则无法接受高等教育的学生。84%的QCC学生是非裔美国人、亚裔或拉丁裔，超过50%的学生来自收入低于2.5万美元的家庭。每学期，有超过三分之一的生物科学和地质学系的教师在研究技术和伦理方面指导学生。当学生们开始在STEM领域的职业生涯时，我们的教师对他们的成功投入了极大的精力。该系统的收购将补充校园的本科生研究核心设施，其中部分包括实时PCR设备，FACS机器和共聚焦显微镜。核心设施中的所有仪器都允许教师向学生介绍尖端的实验方法，并帮助他们为进入STEM劳动力做好准备。新获得的LI-COR Odyssey仪器也将成为QCC生物技术计划和新成立的生物学联合计划教育培训的组成部分，从而影响更多的学生。LI-COR奥德赛是QCC及其姊妹校区皇后学院持续研究和教学课程的重要组成部分。它的收购将支持各种不同的项目。这些研究包括(a)细胞间相互作用中STAT3的分子调控，(b)细胞内寄生虫对宿主氨基酸转运蛋白的调控，(c)卷木菌中保守的根发育基因的检测，(d)大肠杆菌中内在未折叠多肽底物对DnaK的抑制，(e)单壁纳米管处理后癌细胞基因表达的研究，(f)刺胞动物行为的分子调控，(g)研究肥胖和相关合并症中的新蛋白，(h) CDX1作为结肠癌对化疗药物敏感性调节因子的作用，以及(i)心血管健康相关研究。教师和学生将通过同行评议的期刊出版物以及在区域和国家举行的专业和科学会议上传播使用该仪器产生的数据。因此，采购这个系统将为QCC最近招募的早期职业研究人员及其学生开辟新的研究领域。该奖项反映了美国国家科学基金会的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。