Collaborative Research: Development of a New Instrument for Underway High-Resolution Estimates of Aquatic Gross Primary Production

合作研究：开发一种新仪器，用于正在进行的水生初级生产力的高分辨率估算

• 批准号: 2123199
• 负责人: David Nicholson
• 金额: $ 39.02万
• 依托单位: Woods Hole Oceanographic Institution
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-01 至 2025-08-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2123199&HistoricalAwards=false
• 关键词: Collaborative; Research; Development; New; Instrument

Photosynthesis is arguably the most critical biological process of our climate system. It is also central to the trophic and metabolic states of ecosystems. Refining estimates of photosynthesis is therefore critical to our understanding of biogeochemistry and the global climate. Spatial variability of photosynthesis is difficult to resolve due to limitations in technology; the development of high-resolution instrumentation is needed to close this gap. To characterize the geographical distribution of marine photosynthesis, this research will develop an instrument for underway shipboard measurements of aquatic photosynthesis by spectral monitoring of dissolved oxygen (O2) isotopes. Named GOPTICAS (Gross Oxygen Production by Triple Isotope Cavity ringdown laser Absorption Spectroscopy), this instrument will allow for high-resolution observations of photosynthetic O2 evolution, or gross primary production (GPP) by monitoring the dissolved O2 triple isotope signature by wavelength-scanned cavity ring-down absorption spectroscopy (CRDS). The new instrument will extract dissolved O2 into a dried gas stream, then convert it to water (H2O) in a fuel cell downstream of which the isotopic signature of the produced water (H2O) will be monitored with a CRDS. GOPTICAS will 1) provide high-resolution, near real-time and precise mapping of GPP rates to guide sampling strategies, 2) allow for simple field deployment, and finally 3) can be duplicated by other research groups interested in GPP in marine and freshwater ecosystems. Measurements directly in the field will also limit artifacts associated with sample handling and long-term preservation. The instrument will be tested on short cruises aboard the R/V Shearwater in Pamlico Sound and in the Gulf Stream, and on Northeast Shelf LTER cruises. GOPTICAS will improve the characterization of the biogeography of marine photosynthesis, a microbial process with profound biogeochemical implications. Once developed and validated, GOPTICAS will help constrain the global carbon budget and ultimately improve our mechanistic understanding of the feedbacks and coupling between photosynthesis and climate and, in the process, inform and challenge models of ocean biogeochemistry. Finally, this study will be used as a platform to 1) broaden participation in STEM among a diverse population of High School and Undergraduate students through creation of research-based, experiential learning opportunities in engineering and environmental science, 2) teach our lab members how to communicate climate science to the public, and 3) collectively engage students and the public in a discourse about climate change, one of the most pressing issues facing our society in the 21st century.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

光合作用可以说是我们气候系统中最关键的生物过程。它也是生态系统营养和代谢状态的核心。因此，精确估算光合作用对我们理解地球化学和全球气候至关重要。由于技术的限制，光合作用的空间变异性很难解决;需要开发高分辨率仪器来缩小这一差距。为了表征海洋光合作用的地理分布，本研究将开发一种通过溶解氧（O2）同位素的光谱监测进行船舶测量的水生光合作用的仪器。名为GOPTICAS（总氧产量三重同位素腔衰荡激光吸收光谱），该仪器将允许光合O2进化的高分辨率观测，或总初级生产（GPP）通过监测溶解O2三重同位素签名波长扫描腔衰荡吸收光谱（CRDS）。新仪器将溶解的O2提取到干燥的气流中，然后在燃料电池中将其转化为水（H2O），在燃料电池下游，将用CRDS监测采出水（H2O）的同位素特征。 GOPTICAS将1）提供高分辨率，近实时和精确的GPP率映射，以指导采样策略，2）允许简单的现场部署，最后3）可以由其他对海洋和淡水生态系统中的GPP感兴趣的研究小组复制。直接在现场进行测量也将限制与样品处理和长期保存相关的伪影。该仪器将在帕姆利科湾和墨西哥湾流的R/V Shearwater号上进行短途巡航，并在东北大陆架LTER巡航中进行测试。GOPTICAS将改进海洋光合作用的生物地理学特征，这是一个具有深刻生物地球化学影响的微生物过程。 一旦开发和验证，GOPTICAS将有助于限制全球碳预算，并最终提高我们对光合作用和气候之间的反馈和耦合的机械理解，并在此过程中为海洋生物地球化学模型提供信息和挑战。最后，这项研究将被用作一个平台，以1）通过在工程和环境科学中创造基于研究的体验式学习机会，扩大高中和本科学生的多元化人群对STEM的参与，2）教我们的实验室成员如何向公众传播气候科学，3）集体参与学生和公众关于气候变化的讨论，这个奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。