STTR Phase I: Rapid-Release Cell Culture Platform for Flow Cytometry

这一小型企业技术转让(STTR)第一阶段项目的更广泛影响是实现新的健康诊断，推进个性化医疗，并加快各种疾病的研究，包括多种癌症。许多用于诊断疾病的方法都受到处理患者样本的方法的限制。常用的方法可能会破坏或破坏样本中存在的疾病标志物，导致对诊断可靠性和准确性的担忧。拟议的产品平台可以通过保留疾病标记物以及增加产量来解决这一痛点，从而获得更高保真度的实验结果，并最终增强诊断药物。拟议的项目将推动一种变革性生物技术平台的发展，该平台以非破坏性的方式将贴壁的哺乳动物细胞从细胞盘表面分离。现有的方法可以破坏这些细胞，并且细胞产量高度不同，这极大地限制了疾病诊断和治疗发现的临床应用。为了实现其作为现有方法的极具说服力的替代方案的承诺，该项目将解决以下技术障碍：1)确定材料的最佳组成和设计，2)验证平台对细胞的影响，3)使用有利于制造目的的制造方法，以及4)将其性能与现有的用于贴壁细胞的流式细胞术方法进行基准比较。该奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。

The broader impact of this Small Business Technology Transfer (STTR) Phase I project is to enable new health diagnostics, advance personalized medicine, and accelerate research for various diseases, including many types of cancers. Many methods used to diagnose disease are limited by the methods used to process patient samples. Commonly used processes can disrupt or destroy disease markers present in samples, leading to concerns about diagnostic dependability and accuracy. The proposed product platform can address this pain point by retaining disease markers as well as increasing yields which results in higher-fidelity experimental results and ultimately, enhances diagnostic medicine.The proposed project will advance the development of a transformational biotechnology platform which non-destructively detaches adherent mammalian cells from a cell dish surface. Existing methods can damage these cells and are highly varied in cell yields which greatly limit clinical applications for disease diagnosis and therapeutic discovery. To fulfill its promise as a highly compelling alternative to existing methods, the following technical hurdles will be addressed in this project: 1) determining the optimal composition and design of the material, 2) validating the effects of the platform on cells, 3) using fabrication methods conducive to manufacturing purposes, and 4) benchmarking its performance against existing Flow Cytometry methods for adherent cells.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.