Solid-State NMR and DNP Studies of Diatom Biosilica:Organic Matrices and the Silica/Organic Interface
硅藻生物二氧化硅的固态 NMR 和 DNP 研究:有机基质和二氧化硅/有机界面
基本信息
- 批准号:249432230
- 负责人:
- 金额:--
- 依托单位:
- 依托单位国家:德国
- 项目类别:Research Units
- 财政年份:2014
- 资助国家:德国
- 起止时间:2013-12-31 至 2020-12-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The present project aims to investigate two overarching questions:(i) What is the structure of the silica/organic interface in biosilica and which interactions are present at this interface?(ii) What is the structure of the novel membrane protein Sin1? To answer question (i), the Brunner group will build its work on the discoveries made during the first funding period. They will investigate the interactions between organic biosilica constituents and silicic acid/silica as well as between soluble and insoluble organic biosilica constituents (e.g., the interactions between long-chain polyamines (LCPA) and insoluble matrices). Solid-state NMR spectroscopy will be employed, if necessary DNP-assisted (collaboration with the Baldus group). The investigations will encompass spectroscopic studies of the intact, extracted diatom biosilica ([13C,15N,29Si]-enriched samples) and in vitro studies. The investigation of biosilica will be focused to the species Cyclotella cryptica and Thalassiosira pseudonana. Biosilica from T. pseudonana will be studied with respect to the presence of structured proteins. In the case of C. cryptica, the insoluble, chitin-containing organic matrices found by the Kröger group (SP-1) are of special interest. The in vitro studies will encompass extended investigations of LCPA-silica composites using site-specifically 13C/15N-labeled synthetic LCPA from the Geyer group (SP-6). Silicification will be performed using 29Si-enriched silicic acid under physiologically relevant conditions (pH 5-6, ambient temperature). Such composites will also be synthesized in the presence of insoluble organic matrices, e.g., from C. cryptica (with and without LCPA). Calculations carried out by the Cuniberti group (SP-8) will provide further insight and help interpreting the spectroscopic data.To answer question (ii), the Baldus group will perform studies to determine the 3D structure of the novel membrane protein Sin1 as well as its aggregation behavior. This protein was discovered in the first funding period by the Kröger and Shevchenko groups (SP-1 and SP-3). Solid-state NMR spectroscopy at very high field (currently up to 950 MHz proton resonance frequency, in the near future at 1.2 GHz) and - if necessary - DNP-assisted solid-state NMR spectroscopy (800 MHz) will be employed by the Baldus group in order to answer the following questions: How are soluble Sin1 aggregates structurally organized? How does Sin1 interact with membranes? At the same time, research will be conducted in close collaboration with the Kröger group (SP-3) and the Steinem group (see project SP-7).
本项目旨在研究两个主要问题:(I)生物二氧化硅中二氧化硅/有机界面的结构是什么?在这个界面上存在哪些相互作用?(Ii)新型膜蛋白SIN1的结构是什么?为了回答问题(I),布伦纳小组将在第一个资助期内的发现的基础上开展工作。他们将研究有机生物硅组分与硅酸/二氧化硅之间的相互作用,以及可溶和不溶有机生物硅组分之间的相互作用(例如,长链多胺(LCPA)与不溶基质之间的相互作用)。如有必要,将使用固体核磁共振光谱学,并在DNP的协助下(与巴尔德斯小组合作)。调查将包括完整的、提取的硅藻生物硅胶(富含[13C,15N,29Si]的样品)的光谱研究和体外研究。生物硅胶的研究将集中在隐环藻和假海链藻两个物种。关于结构蛋白的存在,将对假毛虫的生物硅进行研究。在隐藻的情况下,Kröger组(SP-1)发现的不溶的、含有甲壳素的有机基质特别令人感兴趣。体外研究将包括使用Geyer集团(SP-6)的13C/15N标记的合成LCPA对LCPA-二氧化硅复合材料的扩展研究。硅化将使用29Si富硅酸在生理相关条件下(pH 5-6,常温)进行。这种复合材料也将在不溶有机基质存在的情况下合成,例如,从隐形藻类(有和没有LCPA)合成。库尼伯蒂小组(SP-8)进行的计算将提供进一步的洞察和帮助解释光谱数据。为了回答问题(II),巴尔德斯小组将进行研究,以确定新型膜蛋白SIN1的三维结构及其聚集行为。这种蛋白质是Kröger和Shevchenko小组(SP-1和SP-3)在第一个资助期发现的。巴尔杜斯小组将使用非常高场下的固态核磁共振波谱(目前高达950兆赫的质子共振频率,不久的将来将达到1.2兆赫),并--如有必要--DNP辅助的固态核磁共振波谱(800兆赫),以回答以下问题:可溶性SIN1聚集体是如何在结构上组织的?SIN1是如何与膜相互作用的?同时,将与Kröger小组(SP-3)和施泰纳姆小组(见SP-7项目)密切合作进行研究。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Professor Dr. Marc Baldus其他文献
Professor Dr. Marc Baldus的其他文献
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{{ truncateString('Professor Dr. Marc Baldus', 18)}}的其他基金
Solid-state NMR Spectroscopy on Biomolecular Complexes at Lowest Temperatures and Highest Fields
最低温度和最高场强下生物分子复合物的固态核磁共振波谱分析
- 批准号:
17752067 - 财政年份:2005
- 资助金额:
-- - 项目类别:
Research Grants
Struktur des Membran-ständigen Fumaratsensors DcuS von Escherichia coli
大肠杆菌膜永久富马酸传感器 DcuS 的结构
- 批准号:
5325910 - 财政年份:2001
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