ERI: Quantifying Inositol Phosphate Dephosphorylation to Understand the Role of Recalcitrant Organic Phosphorus Forms on Harmful Algal Blooms in Freshwater Systems

ERI：量化肌醇磷酸盐去磷酸化，以了解顽固有机磷形式对淡水系统中有害藻类繁殖的作用

• 批准号: 2138520
• 负责人: Francisco Cubas
• 金额: $ 20万
• 依托单位: Georgia Southern University Research and Service Foundation, Inc
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-06-01 至 2025-05-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2138520&HistoricalAwards=false
• 关键词: ERI; Quantifying; Inositol; Phosphate; Dephosphorylation

Rapid urbanization and agricultural intensification in the United States and worldwide have led to a widespread nutrient enrichment, a process commonly referred to as eutrophication, in surface water systems including lakes, rivers, and estuaries. In phosphorus-limited surface water systems, eutrophication has been shown to cause a decrease in water quality, oxygen depletion, a loss of aquatic biota, and the occurrence of harmful algal blooms (HABs). Frequent HABs have an adverse impact in communities that rely on surface water bodies for drinking water, fishing, recreational activities, and tourism. The overarching goal of this ERI project is to investigate the role and impact of recalcitrant organic phosphorus species on the occurrence of HABs in surface water systems. To advance this goal, the Principal Investigator of this ERI project proposes to focus on inositol phosphate (IP) as an important and representative form of recalcitrant organic phosphorus, and its impact on the occurrence of HABS. Two key objectives of the proposed research are to 1) quantify the formation of soluble reactive phosphorus (orthophosphate) from IP in surface water systems and 2) assess how reactive orthophosphate species generated from the dephosphorylation of IP initiate and sustain HABs. The successful completion of this project will benefit society through the development of new fundamental knowledge that could guide the control and mitigation of HABs. Further benefits to society will be achieved through student education and training including the mentoring of two graduate students at Georgia Southern University.The role of inorganic phosphorus on the occurrence, frequency, and intensity of harmful algal blooms (HABs) in surface water systems is well understood. The role and impact of organic phosphorus on HABs have also been established. However, a fundamental understanding and quantification of the bioavailability of organic phosphorus (Po) and its role in promoting and sustaining HABs remains elusive. This ERI project will address these critical knowledge gaps. More specifically, the Principal Investigator (PI) proposes to focus on the evaluation and quantification of the contribution of inositol phosphate (IP), an important and representative recalcitrant Po, to the total pool of orthophosphate (OP) and other soluble reactive phosphorus (SRP) species that fuels and sustain HABs. The specific objectives of the research are to: 1) Quantify the amount of Po produced from the microbial mineralization of IP in surface water systems; 2) Assess the nutritive potential of IP through evaluations of the relationships between IP biodegradability and its role in initiating and sustaining HABs via measurements of the rates of phosphorus acquisition by algae; and 3) Uncover the physical-chemical properties of the environmental media (i.e., OP and total phosphorus (TP) ratios, pH, redox potential, and IP mobility) in which IP is preferentially mineralized. The successful completion of this project has potential for transformative impact through the generation of new knowledge and data to advance the fundamental understanding of the role and impact of IP on the occurrence and persistence of HABs in surface water systems. To implement the education and outreach goals of this ERI project, the PI will develop an outreach program for stakeholders involved in nutrient management to discuss the role of organic phosphorous on eutrophication with the goal of improving P control strategies to prevent HABs. In addition, the PI plans to integrate the research findings into the Environmental Engineering undergraduate/graduate courses that he is currently teaching at Georgia Southern University.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

美国和世界各地的快速城市化和农业集约化导致了广泛的营养富集，这一过程通常被称为富营养化，包括湖泊，河流和河口在内的地表水系统。在磷有限的地表水系统中，富营养化已被证明会导致水质下降、氧气耗尽、水生生物区系丧失和有害藻华的发生。 频繁发生的赤潮对依赖地表水体提供饮用水、捕鱼、娱乐活动和旅游业的社区产生不利影响。该ERI项目的总体目标是调查柠檬酸有机磷物种对地表水系统中有害生物发生的作用和影响。为了推进这一目标，本ERI项目的主要研究者建议重点关注肌醇磷酸（IP）作为一种重要的和有代表性的柠檬酸有机磷形式，及其对HABS发生的影响。拟议研究的两个关键目标是：1）量化地表水系统中IP中可溶性活性磷（正磷酸盐）的形成; 2）评估IP脱磷酸化产生的活性正磷酸盐物种如何启动和维持HABs。该项目的成功完成将通过发展新的基本知识来指导控制和减缓有害赤潮，从而造福社会。通过学生教育和培训，包括指导格鲁吉亚南方大学的两名研究生，将进一步造福社会。无机磷对地表水系统中有害藻华（HABs）的发生、频率和强度的作用已得到充分理解。有机磷对有害藻华的作用和影响也已确定。 然而，有机磷（Po）的生物利用度及其在促进和维持赤潮中的作用的基本理解和量化仍然是难以捉摸的。这个ERI项目将解决这些关键的知识差距。更具体地说，首席研究员（PI）建议侧重于评估和量化磷酸肌醇（IP）的贡献，这是一种重要的和具有代表性的磷，对正磷酸盐（OP）和其他可溶性活性磷（SRP）物种的总池的贡献，这些物种为有害生物提供燃料和维持有害生物。本研究的具体目标是：1）定量测定IP在地表水系统中微生物矿化产生的Po的量：2）通过测量藻类对磷的吸收速率，评价IP的生物降解性与其在引发和维持赤潮中的作用之间的关系，评价IP的营养潜力;以及3）揭示环境介质的物理化学性质（即，OP和总磷（TP）的比例，pH值，氧化还原电位，和IP的流动性），其中IP优先矿化。这一项目的成功完成有可能产生变革性影响，因为它产生了新的知识和数据，可以促进人们从根本上了解综合方案对地表水系统中有害生物的发生和持久性的作用和影响。为了实现ERI项目的教育和推广目标，PI将为参与营养物管理的利益相关者制定一个推广计划，以讨论有机磷对富营养化的作用，目的是改善磷控制策略，以防止HABs。此外，PI计划将研究成果整合到他目前在格鲁吉亚南方大学教授的环境工程本科/研究生课程中。该奖项反映了NSF的法定使命，并被认为值得通过使用基金会的智力价值和更广泛的影响审查标准进行评估来支持。