PFI-TT: Non-enzymatic harvesting of cell cultures

PFI-TT：细胞培养物的非酶收获

基本信息

批准号：
2141138
负责人：
Sergiy Minko
金额：
$ 25万
依托单位：
University of Georgia Research Foundation Inc
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2022
资助国家：
美国
起止时间：
2022-04-15 至 2025-03-31
项目状态：
未结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=2141138&HistoricalAwards=false
关键词：
PFI TT Non enzymatic harvesting

项目摘要

The broader impact/commercial potential of this Partnerships for Innovation - Technology Translation (PFI-TT) project is to address an unmet need in the regenerative medicine and therapeutic cell market. If successful, the proposed technology will boost cell and drug manufacturing for biomedical applications. The proposed program will provide training opportunities in the areas of engineering of functional soft materials and entrepreneurial skills for the commercialization and development of startups. The proposed project aims at developing robust and cost-efficient cell culture dish inserts with thermos-responsive coatings for enzyme-free detachment of high-quality cultured cells with no enzyme contaminations nor damage to the cells and their environment. The product will secure improved efficiency and productivity of cell manufacturing along with the high quality of the products for end-user applications. The research program is designed to address knowledge gaps and technical barriers through the (i) development of a cost-efficient scalable method for the fabrication of micro-structured thermo-responsive thin-film coatings; (ii) identification of the optimal dimensions of the adhesive and repulsive patterns to enable adhesion, proliferation and enzyme-free harvesting of different cell lines; and (iii) study of the cultured and detached cells to assess potential cell damage for optimization of the adhesion level.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

这种创新合作伙伴关系的更广泛的影响/商业潜力 - 技术翻译（PFI -TT）项目是解决再生医学和治疗细胞市场的未满足需求。如果成功，提出的技术将促进生物医学应用的细胞和药物制造。拟议的计划将在功能软材料的工程领域和创业技能方面提供培训机会，以实现创业的商业化和开发。拟议的项目旨在使用热响应式涂层来开发稳健且具有成本效益的细胞培养皿插入，以无酶脱离酶脱离酶，没有酶污染，也不会损害细胞及其环境。该产品将确保细胞制造的提高效率和生产力以及最终用户应用产品的高质量。该研究计划旨在通过（i）开发一种可扩展的可扩展方法来解决知识差距和技术障碍，以制造微观结构的热响应性薄膜涂层；（ii）鉴定粘合剂和排斥模式的最佳维度，以实现不同细胞系的粘附，增殖和无酶收获；（iii）研究培养和分离的细胞以评估潜在的细胞损害以优化粘附水平。该奖项反映了NSF的法定任务，并被认为是值得通过基金会的知识分子优点和更广泛的影响评估的评估来支持的。