CAREER: Multiplexing Light-Field Microscopy for Cell Biological Research

该奖项全部或部分由《2021年美国救援计划法案》（公法117-2）资助。佐治亚理工学院获颁生物光子学和高级显微镜综合研究和教育基础设施创新奖。这个CAREER项目将促进成像科学技术的发现，跨学科基础设施的发现和培训的转变，以及科学界和未被充分代表的群体更广泛地参与STEM。该项目旨在通过技术突破和基础生物学的新知识以及最终的转化研究，改变依赖于传统生物光子方法的生物研究。为了扩大这项工作的教育影响，PI将(1)通过校园或在线资源优先招募女性和少数民族本科生和研究生参与该项目；(2)通过组织亚特兰大生物光子学和先进光学显微镜国际学校，将研究和教育结合起来；(3)开发创新的教学方法，加强问题教育，提高公众科学素养。(4)通过加强学校-实验室互动和生物光子学夏令营，让代表性不足和经验丰富的STEM群体参与进来。这些努力将影响下一代生物学家、成像工程师和专业人员的培训，并促进不同学科的研究和教育的相互促进。在过去的几十年里，荧光显微镜已经成为生物研究中最重要和最具信息量的驱动力之一。因此，越来越多地要求基础设施的发展，以应付未满足的生物发现需求。因此，该项目解决了显微镜创新和细胞生物学研究之间的关键接口。特别是，该研究将通过开发多路光场仪器和方法- mux-LFM来推进活细胞成像。multi - lfm平台正致力于改造当前的细胞显微镜基础设施，通过统一的架构实现功能、结构和种群活细胞的查询。这一基础设施的进步将使广泛的细胞生物学研究朝着分子、亚细胞和细胞程序的系统级理解方向发展。研究结果将激发未来的技术创新，激发重要的生物学见解，并为更广泛的科学、工程和技术突破创造方法途径。该奖项反映了美国国家科学基金会的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

This award is funded in whole or in part under the American Rescue Plan Act of 2021 (Public Law 117-2).An award is made to Georgia Institute of Technology to establish integrated research and educational infrastructure innovation in biophotonics and advanced microscopy. This CAREER project will catalyze the discovery of imaging science and technology, the transformation of cross-disciplinary infrastructure for discovery and training, and the broader participation of scientific communities and underrepresented groups in STEM. The project seeks to transform biological investigations relying on conventional biophotonic methods, through technical breakthroughs and new knowledge in fundamental biology and, ultimately, translational research. To broaden the educational impacts of the work, the PI will (1) prioritize and recruit women and minority undergraduate and graduate students to work on this project through on-campus or online resources, (2) integrate research and education by organizing an Atlanta international school on biophotonics and advanced optical microscopy, (3) develop innovative teaching and learning methods to enhance problem-based education and improve the public scientific literacy, and (4) engage underrepresented and veteran groups in STEM by enhancing school-lab interactions and through a summer camp on biophotonics. These efforts will impact the training of next-generation biologists, imaging engineers, and professionals and promote cross-fertilization of research and education from diverse disciplines. Over the past decades, fluorescence microscopy has emerged as one of the most vital and informative driving forces for biological research. Infrastructure developments have therefore been increasingly demanded to cope with the unmet needs of biological discovery. In response, this project addresses the critical interface between microscopy innovations and cell biological research. In particular, the research will advance live-cell imaging by developing multiplexing light-field instrumentation and methods – mux-LFM. The mux-LFM platform is being pursued to transform the current cell microscopy infrastructure, realizing functional, structural, and populational live-cell interrogations through a unified architecture. This infrastructural advance will enable broad cell biological research toward a systems-level understanding of molecular, subcellular, and cellular programs. The results will inspire future technology innovations, stimulate significant biological insights, and create methodological pathways for broader science, engineering, and technology breakthroughs.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.