CAREER: Multiplexing Light-Field Microscopy for Cell Biological Research

职业：用于细胞生物学研究的多重光场显微镜

• 批准号: 2145235
• 负责人: Shu Jia
• 金额: $ 79.32万
• 依托单位: Georgia Tech Research Corporation
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-02-01 至 2027-01-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2145235&HistoricalAwards=false
• 关键词: CAREER; Multiplexing; Light; Field; Microscopy

This award is funded in whole or in part under the American Rescue Plan Act of 2021 (Public Law 117-2).An award is made to Georgia Institute of Technology to establish integrated research and educational infrastructure innovation in biophotonics and advanced microscopy. This CAREER project will catalyze the discovery of imaging science and technology, the transformation of cross-disciplinary infrastructure for discovery and training, and the broader participation of scientific communities and underrepresented groups in STEM. The project seeks to transform biological investigations relying on conventional biophotonic methods, through technical breakthroughs and new knowledge in fundamental biology and, ultimately, translational research. To broaden the educational impacts of the work, the PI will (1) prioritize and recruit women and minority undergraduate and graduate students to work on this project through on-campus or online resources, (2) integrate research and education by organizing an Atlanta international school on biophotonics and advanced optical microscopy, (3) develop innovative teaching and learning methods to enhance problem-based education and improve the public scientific literacy, and (4) engage underrepresented and veteran groups in STEM by enhancing school-lab interactions and through a summer camp on biophotonics. These efforts will impact the training of next-generation biologists, imaging engineers, and professionals and promote cross-fertilization of research and education from diverse disciplines. Over the past decades, fluorescence microscopy has emerged as one of the most vital and informative driving forces for biological research. Infrastructure developments have therefore been increasingly demanded to cope with the unmet needs of biological discovery. In response, this project addresses the critical interface between microscopy innovations and cell biological research. In particular, the research will advance live-cell imaging by developing multiplexing light-field instrumentation and methods – mux-LFM. The mux-LFM platform is being pursued to transform the current cell microscopy infrastructure, realizing functional, structural, and populational live-cell interrogations through a unified architecture. This infrastructural advance will enable broad cell biological research toward a systems-level understanding of molecular, subcellular, and cellular programs. The results will inspire future technology innovations, stimulate significant biological insights, and create methodological pathways for broader science, engineering, and technology breakthroughs.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该奖项全部或部分由2021年美国救援计划法案（公法117-2）资助。该奖项授予格鲁吉亚理工学院，以建立生物光子学和先进显微镜的综合研究和教育基础设施创新。这个CAREER项目将促进成像科学和技术的发现，跨学科基础设施的发现和培训的转变，以及科学界和STEM中代表性不足的群体的更广泛参与。该项目旨在通过基础生物学的技术突破和新知识以及最终的转化研究，改变依赖传统生物光子方法的生物学研究。为了扩大这项工作的教育影响，PI将（1）优先考虑并招募女性和少数民族本科生和研究生通过校园或在线资源参与该项目，（2）通过组织生物光子学和先进光学显微镜的亚特兰大国际学校来整合研究和教育，（三）发展创新的教与学方法，加强以问题为本的教育，提高公众的科学素养，以及（4）通过加强学校与实验室的互动和生物光子学夏令营，让STEM中代表性不足的群体和退伍军人群体参与进来。这些努力将影响下一代生物学家，成像工程师和专业人员的培训，并促进来自不同学科的研究和教育的交叉。在过去的几十年里，荧光显微镜已经成为生物研究中最重要和最具信息量的驱动力之一。因此，越来越多地要求发展基础设施，以科普生物发现的未满足需求。作为回应，该项目解决了显微镜创新和细胞生物学研究之间的关键接口。特别是，该研究将通过开发多路复用光场仪器和方法- mux-LFM来推进活细胞成像。mux-LFM平台正在寻求改变当前的细胞显微镜基础设施，通过统一的架构实现功能，结构和群体活细胞询问。这一基础设施的进步将使广泛的细胞生物学研究能够实现对分子，亚细胞和细胞程序的系统水平的理解。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Cationic cholesterol-dependent LNP delivery to lung stem cells, the liver, and heart

• DOI: 10.1073/pnas.2307801120
• 发表时间: 2024-03
• 期刊: Proceedings of the National Academy of Sciences of the United States of America
• 影响因子: 11.1
• 作者: Afsane Radmand;Hyejin Kim;Jared P Beyersdorf;Curtis N Dobrowolski;Ryan Zenhausern;Kalina Paunovska;Sebastian G. Huayamares;Xuanwen Hua;Keyi Han;David Loughrey;Marine Z. C. Hatit;Ada Del Cid;Huanzhen Ni;Aram Shajii;Andrea Li;Abinaya Muralidharan;H. Peck;Karen E Tiegreen;Shu Jia;P. Santangelo;J. Dahlman

Volumetric live-cell autofluorescence imaging using Fourier light-field microscopy

使用傅里叶光场显微镜进行体积活细胞自发荧光成像

• DOI: 10.1364/boe.495506
• 发表时间: 2023
• 期刊: Biomedical Optics Express
• 影响因子: 3.4
• 作者: Ling, Zhi;Han, Keyi;Liu, Wenhao;Hua, Xuanwen;Jia, Shu
• 通讯作者: Jia, Shu