Collaborative Research: In vivo Deep Tissue Imaging with Ultrafast, Volumetric Super-Resolution Microscopy

Jia, Shu / Ying, Leslie 1604565 / 1604531 这项工作将促进对大脑的发现和理解，改善医疗保健，并加强研究和教育基础设施。该项目将为在生物学和临床研究中更好地了解阿尔茨海默氏症等神经退行性疾病奠定基础。超分辨率光谱学的进步将为转化医学应用提供快速、分子水平、非侵入性、高特异性成像工具。该项目将开发一种新的体积、超快超分辨率显微镜，以克服组织和器官功能体内研究的技术障碍。诸如随机光学重建显微镜（STORM）之类的切换方法依靠单分子成像来重建具有亚衍射极限分辨率的荧光图像。小胶质细胞对 Ab（β 淀粉样蛋白）的反应在患病 (AD) 大脑中发挥着至关重要的作用；最终为临床研究提供工具和解决方案。拟议的超分辨率研究将为小胶质细胞在维持大脑功能和应对病理损伤方面的作用提供新的见解。拟议的工作将为更深入地了解小胶质细胞对神经退行性疾病的反应和影响奠定技术和知识基础。

Jia, Shu / Ying, Leslie 1604565 / 1604531 This work will advance the discovery and understanding of the brain, improve healthcare, and enhance infrastructure for research and education. The project will lay the foundation for a better understanding of neurodegenerative diseases such as Alzheimer's in both biological and clinical investigations. The super-resolution spectroscopy advances will provide fast, molecular level, non-invasive, high specificity imaging tools for translational medical applications.This project will develop a new volumetric, ultrafast super-resolution microscopy to overcome technical barriers for in vivo investigations of tissue and organ functions. Switching methods, such as stochastic optical reconstruction microscopy (STORM) rely on imaging of single molecules to reconstruct fluorescence images with sub-diffraction-limited resolution. The roles of microglia in response to Ab (Amyloid beta) are crucially involved in the diseased (AD) brain; and ultimately provide tools and solutions for clinical investigations. The proposed super-resolution investigation will provide new insights into the role of microglia to the maintenance of brain function and in response to pathological insults. The proposed work will pave the technological and intellectual foundation for deeper understanding of the microglial response and influence to neurodegenerative disorders.