Collaborative Research: In vivo Deep Tissue Imaging with Ultrafast, Volumetric Super-Resolution Microscopy

贾舒/英，Leslie 1604565/1604531这项工作将促进对大脑的发现和理解，改善医疗保健，并加强研究和教育的基础设施。该项目将为在生物学和临床研究中更好地了解阿尔茨海默氏症等神经退行性疾病奠定基础。超分辨率光谱学的进展将为转化医学应用提供快速、分子水平、非侵入性、高特异性的成像工具。本项目将开发一种新的体积、超快超分辨率显微镜，以克服体内组织和器官功能研究的技术障碍。开关方法，如随机光学重建显微镜(STORM)，依赖于单分子成像来重建亚衍射极限分辨率的荧光图像。小胶质细胞在AB(淀粉样β蛋白)反应中的作用在疾病(AD)脑中起着至关重要的作用，并最终为临床研究提供工具和解决方案。这项拟议的超分辨率研究将为小胶质细胞在维持大脑功能和应对病理性侮辱中的作用提供新的见解。这项拟议的工作将为更深入地了解小胶质细胞的反应和对神经退行性疾病的影响奠定技术和智力基础。

Jia, Shu / Ying, Leslie 1604565 / 1604531 This work will advance the discovery and understanding of the brain, improve healthcare, and enhance infrastructure for research and education. The project will lay the foundation for a better understanding of neurodegenerative diseases such as Alzheimer's in both biological and clinical investigations. The super-resolution spectroscopy advances will provide fast, molecular level, non-invasive, high specificity imaging tools for translational medical applications.This project will develop a new volumetric, ultrafast super-resolution microscopy to overcome technical barriers for in vivo investigations of tissue and organ functions. Switching methods, such as stochastic optical reconstruction microscopy (STORM) rely on imaging of single molecules to reconstruct fluorescence images with sub-diffraction-limited resolution. The roles of microglia in response to Ab (Amyloid beta) are crucially involved in the diseased (AD) brain; and ultimately provide tools and solutions for clinical investigations. The proposed super-resolution investigation will provide new insights into the role of microglia to the maintenance of brain function and in response to pathological insults. The proposed work will pave the technological and intellectual foundation for deeper understanding of the microglial response and influence to neurodegenerative disorders.