Solution mediated surface reactions of bioactive rapidly resorbable bone substitute materials and their effect on osteoblastic cell adhesion, intracellular signal transduction mechanismus and apoptosis

使用口腔种植体已成为一种常见的治疗方法，以取代缺牙。然而，牙槽嵴的吸收经常要求在种植体植入之前通过骨增量进行部位开发。使用合成的、可生物降解的骨替代物有利于牙槽嵴增高，因为它避免了自体移植物收获的第二部位手术。与目前临床使用的骨替代材料相比，对更快速生物降解的材料存在相当大的需求。在德国联邦材料研究和测试研究所开发的新的，生物活性的快速吸收磷酸钙。以前，我们证明了这些材料中的几种能够刺激成骨细胞分化，从而增强骨生成。然而，其基本机制尚未得到很好的理解。发展这种理解一直受到可以使用的实验技术不足的阻碍。然而，在杰斐逊大学，新的分子生物学方法最近已经被用于解决骨生成的类似问题，并且它们已经与使用强大的表面分析技术的洞察力相结合。在这里提出的研究计划中，这些先进的分子方法也将与先进的表面分析技术相结合。因此，生物活性的，快速吸收的骨替代物对细胞粘附和细胞内信号传导机制以及成骨细胞凋亡的影响将得到阐明。此外，这些体外数据将与体内研究的结果相关。以这种方式产生的知识，将大大有助于提高我们的生物活性植入材料在分子水平上的组织整合所涉及的过程的理解。

The use of oral implants has become a common treatment to replace missing teeth. However, resorption of the alveolar ridge frequently mandates site development by bone augmentation before implants can be placed. The use of synthetic, biodegradable bone substitutes is advantageous for alveolar ridge augmentation since it avoids second-site surgery for autograft harvesting. Compared to the bone substitute materials, which are currently clinically used, there is a considerable need for more rapidly biodegrading materials. At the German Federal Institute of Materials Research and Testing novel, bioactive rapidly resorbable calcium phosphates were developed. Previously, we demonstrated that several of these materials are able to stimulate osteoblast differentiation thus enhancing osteogenesis. However, the underlying mechanisms are not yet well understood. Developing this understanding has been hampered by the inadequacy of the experimental techniques that could be used. At Jefferson University, however, new molecular biological methods have recently been brought to bear on similar problems of osteogenesis and they have been combined with insight from using powerful surface analysis techniques. In the research program proposed here, these advanced molecular methods will also be applied in combination with advanced surface analysis techniques. Hereby, the effect of bioactive, rapidly resorbing bone substitutes on cell adhesion and intracellular signaling mechanisms as well as apoptosis of osteoblasts will be elucidated. Furthermore, these in vitro data will be correlated with results from an in vivo study. The knowledge generated in this way, will contribute significantly to enhancing our understanding of the processes involved in tissue integration of bioactive implant materials at a molecular level.