Collaborative Research: CAS: Mechanistic Studies on Fe-Type Nitrile Hydration Catalysts
合作研究:CAS:铁型腈水合催化剂的机理研究
基本信息
- 批准号:2204023
- 负责人:
- 金额:$ 39.11万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:2022
- 资助国家:美国
- 起止时间:2022-09-01 至 2025-08-31
- 项目状态:未结题
- 来源:
- 关键词:
项目摘要
With the support of the Chemistry of Life Processes program in the Division of Chemistry, Professors Brian Bennett and Adam Fiedler from Marquette University and Professor Richard Holz from the Colorado School of Mines will investigate the chemical function of the enzyme nitrile hydratase (NHase), which catalyzes the reaction of nitrile compounds with water (hydration) to give the corresponding amides. NHases hydrate a range of natural and synthetic nitriles with strict selectivity under mild conditions. As such, these enzymes have several industrial and pharmaceutical applications, including the removal of chemicals and pesticides from the environment. To expand the biocatalytic potential of NHases, a better understanding of the catalytic mechanism is essential. Professors Bennett, Fiedler and Holz will employ a combination of experimental and computational approaches to detect and probe intermediates generated during the hydration reaction. In addition, the assembly of the enzyme active site, which contains a cobalt or iron ion, will be examined. The project will provide exceptional training for undergraduate and graduate-level scientists. The emphasis on green chemistry and biophysical methods will serve as the basis for community engagement activities. The award will also enhance the research and educational infrastructures at both Marquette University and the Colorado School of Mines. This research project seeks to gain molecular-level insights into NHase catalysis through a combination of kinetic, spectroscopic, biochemical, computational, and X-ray crystallographic methods. The studies will (i) test competing prevailing mechanistic hypotheses and refine the catalytic mechanism by interrogating observed intermediates, (ii) determine the catalytic function of proton transfer and the protonation states of ionizable groups in the active site, and (iii) elucidate steps in the maturation process of the metal-based (Fe or Co) active sites. The insights gained from the studies are expected to aid in the development of green catalytic approaches to hydrolytic reactions in nature and industry.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
在化学系生命过程化学项目的支持下,来自马奎特大学的Brian班尼特教授和Adam Fiedler教授以及来自科罗拉多矿业学院的Richard Holz教授将研究腈水合酶(NHase)的化学功能,该酶催化腈化合物与水(水合)反应生成相应的酰胺。 NHases在温和条件下以严格的选择性水合一系列天然和合成腈。 因此,这些酶具有多种工业和制药应用,包括从环境中去除化学品和农药。 为了扩大NHases的生物催化潜力,更好地理解催化机制是必不可少的。教授班尼特,菲德勒和霍尔茨将采用实验和计算方法相结合,以检测和探测水合反应过程中产生的中间体。 此外,将检查含有钴或铁离子的酶活性位点的组装。 该项目将为本科生和研究生水平的科学家提供特殊培训。 对绿色化学和生物物理方法的强调将作为社区参与活动的基础。 该奖项还将加强马奎特大学和科罗拉多矿业学院的研究和教育基础设施。该研究项目旨在通过动力学,光谱学,生物化学,计算和X射线晶体学方法的组合来获得分子水平的见解。 这些研究将(i)测试竞争盛行的机制假设,并通过询问观察到的中间体来完善催化机制,(ii)确定质子转移的催化功能和活性位点中可电离基团的质子化状态,以及(iii)阐明金属基(Fe或Co)活性位点成熟过程中的步骤。从这些研究中获得的见解有望有助于自然界和工业中水解反应的绿色催化方法的发展。该奖项反映了NSF的法定使命,并被认为值得通过使用基金会的知识价值和更广泛的影响审查标准进行评估来支持。
项目成果
期刊论文数量(2)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Examination of the Catalytic Role of the Axial Cystine Ligand in the Co-Type Nitrile Hydratase from Pseudonocardia thermophila JCM 3095
轴向胱氨酸配体在嗜热假诺卡氏菌 JCM 3095 共型腈水合酶中的催化作用的检查
- DOI:10.3390/catal11111381
- 发表时间:2021
- 期刊:
- 影响因子:3.9
- 作者:Ogutu, Irene R.;St. Maurice, Martin;Bennett, Brian;Holz, Richard C.
- 通讯作者:Holz, Richard C.
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Brian Bennett其他文献
Catalytic role of histidine-114 in the hydrolytic dehalogenation of chlorothalonil by Pseudomonas sp. CTN-3.
组氨酸 114 在假单胞菌百菌清水解脱卤中的催化作用。
- DOI:
- 发表时间:
2024 - 期刊:
- 影响因子:3
- 作者:
Grayson Gerlich;Callie Miller;Xinhang Yang;Karla Diviesti;Brian Bennett;Judith Klein;Richard C Holz - 通讯作者:
Richard C Holz
Cryo-EM captures the coordination of asymmetric electron transfer through a di-copper site in DPOR
冷冻电镜捕捉到通过 DPOR 中的双铜位点进行不对称电子转移的协调作用
- DOI:
10.1038/s41467-025-59158-7 - 发表时间:
2025-04-24 - 期刊:
- 影响因子:15.700
- 作者:
Rajnandani Kashyap;Natalie Walsh;Jaigeeth Deveryshetty;Monika Tokmina-Lukaszewska;Kewei Zhao;Yunqiao J. Gan;Brian M. Hoffman;Ritimukta Sarangi;Brian Bothner;Brian Bennett;Edwin Antony - 通讯作者:
Edwin Antony
Single-molecule studies of asymmetric substrate binding and electron transfer in the nitrogenase-like DPOR complex
- DOI:
10.1016/j.bpj.2021.11.2201 - 发表时间:
2022-02-11 - 期刊:
- 影响因子:
- 作者:
Rahul Chadda;Elliot Corless;Brian Bennett;Edwin Antony - 通讯作者:
Edwin Antony
Genetic Background Heavily Impacts Effects of Diet on Obesity in a Collaborative Cross Population
- DOI:
10.1093/cdn/nzaa058_040 - 发表时间:
2020-06-01 - 期刊:
- 影响因子:
- 作者:
Phoebe Yam;Jody Albright;Melissa VerHague;Brian Bennett - 通讯作者:
Brian Bennett
The Association of Plasma Choline With Growth and Development Among Young Malawian Children Enrolled in an Egg Intervention Trial
- DOI:
10.1093/cdn/nzab045_009 - 发表时间:
2021-06-01 - 期刊:
- 影响因子:
- 作者:
Megan Bragg;Bess Caswell;Charles Arnold;Matthews George;Brian Bennett;Elizabeth Prado;Kenneth Maleta;Christine Stewart - 通讯作者:
Christine Stewart
Brian Bennett的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Brian Bennett', 18)}}的其他基金
Mechanistic Studies on Fe-Type Nitrile Hydration Catalysts
Fe型腈水合催化剂的机理研究
- 批准号:
1808711 - 财政年份:2018
- 资助金额:
$ 39.11万 - 项目类别:
Standard Grant
MRI: Upgrade of an EPR Spectrometer to Serve Marquette University and the Greater Milwaukee Region
MRI:升级 EPR 光谱仪以服务马凯特大学和大密尔沃基地区
- 批准号:
1532168 - 财政年份:2015
- 资助金额:
$ 39.11万 - 项目类别:
Standard Grant
Collaborative Research: Mechanistic Studies on Fe-type Nitrile Hydration Catalysts
合作研究:铁型腈水合催化剂的机理研究
- 批准号:
1410411 - 财政年份:2014
- 资助金额:
$ 39.11万 - 项目类别:
Continuing Grant
Collaborative Research: Mechanistic Studies on Fe-type Nitrile Hydration Catalysts
合作研究:铁型腈水合催化剂的机理研究
- 批准号:
1462201 - 财政年份:2014
- 资助金额:
$ 39.11万 - 项目类别:
Continuing Grant
Collaborative Research: Mechanistic Studies on Fe-type Nitrile Hydration Catalysts
合作研究:铁型腈水合催化剂的机理研究
- 批准号:
1058396 - 财政年份:2011
- 资助金额:
$ 39.11万 - 项目类别:
Standard Grant
相似国自然基金
Research on Quantum Field Theory without a Lagrangian Description
- 批准号:24ZR1403900
- 批准年份:2024
- 资助金额:0.0 万元
- 项目类别:省市级项目
Cell Research
- 批准号:31224802
- 批准年份:2012
- 资助金额:24.0 万元
- 项目类别:专项基金项目
Cell Research
- 批准号:31024804
- 批准年份:2010
- 资助金额:24.0 万元
- 项目类别:专项基金项目
Cell Research (细胞研究)
- 批准号:30824808
- 批准年份:2008
- 资助金额:24.0 万元
- 项目类别:专项基金项目
Research on the Rapid Growth Mechanism of KDP Crystal
- 批准号:10774081
- 批准年份:2007
- 资助金额:45.0 万元
- 项目类别:面上项目
相似海外基金
CAS: Collaborative Research: Ambient Polyvinyl Chloride (PVC) Upgrading Using Earth-Abundant Molecular Electrocatalysts
CAS:合作研究:使用地球上丰富的分子电催化剂升级常温聚氯乙烯 (PVC)
- 批准号:
2347912 - 财政年份:2024
- 资助金额:
$ 39.11万 - 项目类别:
Standard Grant
CAS: Collaborative Research: Ambient Polyvinyl Chloride (PVC) Upgrading Using Earth-Abundant Molecular Electrocatalysts
CAS:合作研究:使用地球上丰富的分子电催化剂升级常温聚氯乙烯 (PVC)
- 批准号:
2347913 - 财政年份:2024
- 资助金额:
$ 39.11万 - 项目类别:
Standard Grant
Collaborative Research: CAS: Exploration and Development of High Performance Thiazolothiazole Photocatalysts for Innovating Light-Driven Organic Transformations
合作研究:CAS:探索和开发高性能噻唑并噻唑光催化剂以创新光驱动有机转化
- 批准号:
2400166 - 财政年份:2024
- 资助金额:
$ 39.11万 - 项目类别:
Continuing Grant
Collaborative Research: CAS: Exploration and Development of High Performance Thiazolothiazole Photocatalysts for Innovating Light-Driven Organic Transformations
合作研究:CAS:探索和开发高性能噻唑并噻唑光催化剂以创新光驱动有机转化
- 批准号:
2400165 - 财政年份:2024
- 资助金额:
$ 39.11万 - 项目类别:
Continuing Grant
Collaborative Research: CAS-Climate: Linking Activities, Expenditures and Energy Use into an Integrated Systems Model to Understand and Predict Energy Futures
合作研究:CAS-气候:将活动、支出和能源使用连接到集成系统模型中,以了解和预测能源未来
- 批准号:
2243099 - 财政年份:2023
- 资助金额:
$ 39.11万 - 项目类别:
Standard Grant
Collaborative Research: CAS-SC: Development of Heavy Atom - Free Photocatalysts for Chemical Reactions
合作研究:CAS-SC:开发用于化学反应的无重原子光催化剂
- 批准号:
2247661 - 财政年份:2023
- 资助金额:
$ 39.11万 - 项目类别:
Standard Grant
Collaborative Research: CAS-SC: Electrochemical Approaches to Sustainable Dinitrogen Fixation
合作研究:CAS-SC:可持续二氮固定的电化学方法
- 批准号:
2247257 - 财政年份:2023
- 资助金额:
$ 39.11万 - 项目类别:
Continuing Grant
CAS: Collaborative Research: Photophysics and Electron Transfer Reactivity of Ion Radical Excited States
CAS:合作研究:离子自由基激发态的光物理学和电子转移反应性
- 批准号:
2246509 - 财政年份:2023
- 资助金额:
$ 39.11万 - 项目类别:
Standard Grant
CAS: Collaborative Research: Separating Electronic and Geometric Effects in Compound Catalysts: Examining Unique Selectivities for Hydrogenolysis on Transition Metal Phosphides
CAS:合作研究:分离复合催化剂中的电子效应和几何效应:检验过渡金属磷化物氢解的独特选择性
- 批准号:
2409888 - 财政年份:2023
- 资助金额:
$ 39.11万 - 项目类别:
Standard Grant
Collaborative Research: CAS-SC: Development of Heavy Atom - Free Photocatalysts for Chemical Reactions
合作研究:CAS-SC:开发用于化学反应的无重原子光催化剂
- 批准号:
2247662 - 财政年份:2023
- 资助金额:
$ 39.11万 - 项目类别:
Standard Grant