Plant anthranoid biosynthesis

植物类蒽醌生物合成

• 批准号: 251406072
• 负责人: Professor Dr. Ludger Beerhues
• 金额: --
• 依托单位: Institut für Pharmazeutische Biologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2014
• 资助国家: 德国
• 起止时间: 2013-12-31 至 2017-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/251406072?language=en
• 关键词: Plant; anthranoid; biosynthesis

Anthranoid-containing plants are medicinally used as laxatives. Biosynthesis of the anthranoid scaffold is believed to be catalyzed by octaketide synthase, a type III polyketide synthase. Three available recombinant enzymes form a linear octaketide intermediate but fail to catalyze its correct cyclization into an anthranoid product. Thus, an accessory cyclase may be involved, as recently detected in cannabinoid biosynthesis. We will carry out biochemical studies using elicitor-treated Cassia bicapsularis cell cultures, whose cell-free extracts are capable of forming anthranoids. Molecular studies will be performed using genome and transcriptome sequences of Hypericum perforatum, which contains the naphthodianthrone hypericin.

含蒽类化合物的植物在医学上用作泻药。据信类蒽酮支架的生物合成是由奥考肽合酶（一种III型聚酮合酶）催化的。三种可用的重组酶形成线性octaketide中间体，但不能催化其正确环化为蒽类产物。因此，可能涉及辅助环化酶，如最近在大麻素生物合成中检测到的。我们将使用激发子处理过的Cassia bicapsularis细胞培养物进行生化研究，其无细胞提取物能够形成类花色素。分子研究将使用基因组和转录组序列的贯叶连翘，其中含有萘二蒽酮金丝桃素。