MRI: Acquisition of a High Pressure Stopped-Flow Instrument for Studying Biological Systems under Extreme Conditions

MRI：购买高压停流仪器，用于研究极端条件下的生物系统

• 批准号: 2213116
• 负责人: George Makhatadze
• 金额: $ 17.48万
• 依托单位: Rensselaer Polytechnic Institute
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-10-01 至 2025-09-30
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2213116&HistoricalAwards=false
• 关键词: MRI; Acquisition; Pressure; Stopped; Flow

An award is made to Rensselaer Polytechnic Institute to support the purchase of high-pressure stopped flow (HPSF) spectrophotometer/fluorimeter system. The instrument is the first of its kind in the US and will be housed in the RPI Analytical Biochemistry Core Facility. This will ensure that the unique cutting-edge capabilities of the instrument will be accessible to undergraduate students, graduate students, and postdoctoral fellows, not only at RPI but also to a broader community in the NY Capital Region and beyond. A number of laboratories will use the instrument to address questions of basic biochemistry, biology and evolutionary adaptations to extreme environments. The instrument will also be used in a number of hands-on workshops that will include undergraduate and graduate students. The participation in these workshops will be extended to the laboratories from NSF-supported Research Coordination Network on Extreme Biophysics (RCN-EB). In addition, the research that is enabled by this instrument will be incorporated in community outreach programs to encourage students into STEM fields and reaches out to talented underrepresented minorities and young women in the NY Capital Region. Hydrostatic pressure is an important environmental variable that plays an essential role in biological adaptation for many extremophilic organisms that inhabit the depth of the oceans and subsurface crusts. The HPSF instrument will support research activities of a group of investigators interested in understanding the molecular details of the effects of high hydrostatic pressure on the biological function and functional dynamics of biological macromolecules. The ability to experimentally monitor enzyme kinetics as a function of temperature and pressure using the HPSF instrument is critical for understanding of how hydrostatic pressure modulates the activity of the enzymes. This in turn will lead to a better understanding of adaptation of organisms to extreme pressures. High-hydrostatic pressure can also influence the energy landscape for protein folding. High-energy states of proteins are implicated in catalysis, binding, aggregation, allostery, and folding, thus connecting the enzymatic function with their dynamic properties. The results of these research efforts will be disseminated through active participation of students and faculty at scientific conferences, symposia and workshops, through peer-reviewed publications and public outreach presentations.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

伦斯勒理工学院获奖，以支持购买高压停流(HPSF)分光光度/荧光仪系统。该仪器是美国第一台此类仪器，将安装在RPI分析生物化学核心设施中。这将确保该仪器独特的尖端能力将为本科生、研究生和博士后研究员所用，不仅在RPI，而且在纽约首都地区和其他地区的更广泛的社区也是如此。一些实验室将使用该仪器来解决基本生物化学、生物学和进化对极端环境的适应问题。该仪器还将用于包括本科生和研究生在内的一些动手研讨会。参加这些讲习班的将扩大到国家科学基金会资助的极端生物物理学研究协调网络(RCN-EB)的实验室。此外，该工具所支持的研究将被纳入社区推广计划，以鼓励学生进入STEM领域，并接触到纽约首都地区才华横溢、代表性不足的少数民族和年轻女性。静水压力是一个重要的环境变量，对生活在海洋深处和地下结壳中的许多极端生物的生物适应起着至关重要的作用。HPSF仪器将支持一组研究人员的研究活动，这些研究人员有兴趣了解高静水压力对生物功能和生物大分子功能动力学的影响的分子细节。使用HPSF仪器对作为温度和压力函数的酶动力学进行实验监测的能力，对于了解静液压如何调节酶的活性至关重要。这反过来将有助于更好地理解生物体对极端压力的适应。高静水压也会影响蛋白质折叠的能量格局。蛋白质的高能态参与催化、结合、聚集、变构和折叠，从而将酶的功能与其动态性质联系起来。这些研究工作的结果将通过学生和教师积极参与科学会议、研讨会和研讨会，通过同行评议的出版物和公共宣传演讲来传播。该奖项反映了NSF的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。