MRI: Acquisition of a High Pressure Stopped-Flow Instrument for Studying Biological Systems under Extreme Conditions

MRI：购买高压停流仪器，用于研究极端条件下的生物系统

• 批准号: 2213116
• 负责人: George Makhatadze
• 金额: $ 17.48万
• 依托单位: Rensselaer Polytechnic Institute
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-10-01 至 2025-09-30
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2213116&HistoricalAwards=false
• 关键词: MRI; Acquisition; Pressure; Stopped; Flow

An award is made to Rensselaer Polytechnic Institute to support the purchase of high-pressure stopped flow (HPSF) spectrophotometer/fluorimeter system. The instrument is the first of its kind in the US and will be housed in the RPI Analytical Biochemistry Core Facility. This will ensure that the unique cutting-edge capabilities of the instrument will be accessible to undergraduate students, graduate students, and postdoctoral fellows, not only at RPI but also to a broader community in the NY Capital Region and beyond. A number of laboratories will use the instrument to address questions of basic biochemistry, biology and evolutionary adaptations to extreme environments. The instrument will also be used in a number of hands-on workshops that will include undergraduate and graduate students. The participation in these workshops will be extended to the laboratories from NSF-supported Research Coordination Network on Extreme Biophysics (RCN-EB). In addition, the research that is enabled by this instrument will be incorporated in community outreach programs to encourage students into STEM fields and reaches out to talented underrepresented minorities and young women in the NY Capital Region. Hydrostatic pressure is an important environmental variable that plays an essential role in biological adaptation for many extremophilic organisms that inhabit the depth of the oceans and subsurface crusts. The HPSF instrument will support research activities of a group of investigators interested in understanding the molecular details of the effects of high hydrostatic pressure on the biological function and functional dynamics of biological macromolecules. The ability to experimentally monitor enzyme kinetics as a function of temperature and pressure using the HPSF instrument is critical for understanding of how hydrostatic pressure modulates the activity of the enzymes. This in turn will lead to a better understanding of adaptation of organisms to extreme pressures. High-hydrostatic pressure can also influence the energy landscape for protein folding. High-energy states of proteins are implicated in catalysis, binding, aggregation, allostery, and folding, thus connecting the enzymatic function with their dynamic properties. The results of these research efforts will be disseminated through active participation of students and faculty at scientific conferences, symposia and workshops, through peer-reviewed publications and public outreach presentations.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

向Rensselaer理工学院颁发了奖励，以支持购买高压停止流量（HPSF）分光光度计/荧光计系统。 该仪器是美国第一个此类工具，将安置在RPI分析生物化学核心设施中。这将确保本科生，研究生和博士后研究员不仅在RPI，而且在纽约首都地区及以后的更广泛的社区中，本科生，研究生和博士后研究员都可以使用该乐器的独特尖端能力。 许多实验室将使用该工具来解决对极端环境的基本生物化学，生物学和进化适应的问题。 该乐器还将用于许多动手讲习班，其中包括本科生和研究生。 这些研讨会的参与将扩展到NSF支持的极端生物物理学研究协调网络（RCN-EB）的实验室。此外，该工具启用的研究将纳入社区外展计划中，以鼓励学生进入STEM领域，并与纽约首都地区的人才不足的少数民族和年轻妇女接触。静液压是重要的环境变量，它在许多居住在海洋和地下地壳深处的极端生物的生物适应中起着至关重要的作用。 HPSF仪器将支持一群有兴趣了解高静水压力对生物大分子生物学功能和功能动力学影响的研究活动的研究活动。 使用HPSF仪器对酶动力学进行实验监测酶动力学的能力对于理解液压压力如何调节酶的活性至关重要。 反过来，这将使人们更好地理解生物体对极端压力的适应。 高静态压力也会影响蛋白质折叠的能量景观。 蛋白质的高能状态与催化，结合，聚集，变构和折叠有关，从而将酶促功能与其动态特性联系起来。 这些研究工作的结果将通过学生和教职员工在科学会议，研讨会和讲习班上的积极参与，通过同行评审的出版物和公共宣传演讲进行分散。该奖项反映了NSF的法定任务，并被认为是通过基金会的智力功能和广泛影响的评估来审查CRETIRIA的评估。