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MRI: Acquisition of a Hybrid Supercritical Chromatography, High-Pressure Chromatograph, Quadrupole-Time-of-Flight Mass Spectrometer (SFC/UHPLC Q-ToF/MS)

MRI：购买混合超临界色谱、高压色谱、四极杆飞行时间质谱仪 (SFC/UHPLC Q-ToF/MS)

基本信息

批准号：
1828782
负责人：
Joseph Genereux
金额：
$ 25.88万
依托单位：
University of California-Riverside
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2018
资助国家：
美国
起止时间：
2018-08-01 至 2021-07-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1828782&HistoricalAwards=false
关键词：
MRI Acquisition Hybrid Supercritical Chromatography

项目摘要

This award is supported by the Major Research Instrumentation and the Chemistry Research Instrumentation programs. Professor Joseph Genereux from University of California Riverside and colleague Ming Tang have acquired a hybrid supercritical chromatography, ultrahigh pressure liquid chromatograph, quadrupole time-of-flight mass spectrometer (SFC/UHPLC Q-ToF/MS). In general, mass spectrometry (MS) is one of the key analytical methods used to identify and characterize small quantities of chemical species in complex matrices. In a typical experiment, the components flow into a mass spectrometer where they are ionized and the ion masses are measured. This highly sensitive technique allows detection and determination of the structure of molecules in a complex mixture. An instrument with a supercritical chromatograph provides additional structural identification power by separating mixtures of compounds using high pressures and temperature before they reach the mass spectrometer. In the ToF method the mass-to-charge ratio of an ion is determined by the way of a time measurement in which ions are accelerated by an electric field of known strength. The acquisition strengthens the research infrastructure at the University and regional area. The instrument broadens participation by involving diverse students in research and research training using this modern analytical technique. The instrument serves as a tool for broadening the participation of women and underrepresented minorities in science and engineering research at this Hispanic Serving Institution.The award of this unique mass spectrometer is aimed at enhancing research and education at all levels. It especially allows the use of electrophilic foot printing to identify protein conformational changes in complex media and the creation of branched dendrimeric architectures for energy transmission. The mass spectrometer aids the characterization of non-covalent interactions between proteins and structured or modified nucleic acids and the identification of aspartic acid epimerization in the aging proteome. The instrument is also used to study self-sorting, supramolecular catalysis and molecular dynamics as well as to investigate serine peptide assembly. The mass spectrometer also serves researchers studying structure and mechanism in tryptophan synthase and exploring olefin polymerization catalysts with ligands featuring weakly coordinating carborane anions. The instrumentation is also used in analyzing protein biogenesis and protein quality, using metabolomics for deciphering t-cell heterogeneity and using asymmetric methods and synthesis strategies for the study of neuroprotective limonoids. It is also employed to study effects of post-transcriptional and post-translational modifications on the dynamics of eukaryotic translation initiation and to obtain entioselectivity from gold(III) complexes.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该奖项由主要研究仪器和化学研究仪器项目支持。来自加州大学河滨分校的Joseph Genereux教授和他的同事Ming Tang获得了一台混合超临界色谱、超高压液相色谱仪、四极杆飞行时间质谱仪（SFC/UHPLC Q-ToF/MS）。一般来说，质谱（MS）是用于鉴定和表征复杂基质中少量化学物质的关键分析方法之一。在一个典型的实验中，这些成分流入质谱仪，在那里它们被电离，离子质量被测量。这种高度灵敏的技术可以检测和确定复杂混合物中的分子结构。带有超临界色谱仪的仪器在到达质谱仪之前通过高压和高温分离化合物混合物，提供额外的结构识别能力。在ToF方法中，离子的质荷比是通过一种时间测量的方法来确定的，在这种方法中，离子被已知强度的电场加速。此次收购加强了大学和地区的研究基础设施。该仪器通过让不同的学生参与使用这种现代分析技术的研究和研究训练，扩大了参与范围。该文书是扩大妇女和代表性不足的少数民族参与本西班牙裔服务机构科学和工程研究的一个工具。这种独特的质谱仪的奖励旨在加强各级的研究和教育。它特别允许使用亲电足迹来识别复杂介质中的蛋白质构象变化和分支树突结构的创建，以进行能量传输。质谱计有助于表征蛋白质与结构或修饰的核酸之间的非共价相互作用，并鉴定老化蛋白质组中的天冬氨酸外显体化。该仪器还用于研究自分选、超分子催化和分子动力学，以及研究丝氨酸肽的组装。质谱仪还可用于研究色氨酸合成酶的结构和机理，探索以弱配位碳硼烷阴离子为配体的烯烃聚合催化剂。该仪器还用于分析蛋白质的生物发生和蛋白质质量，使用代谢组学来破译t细胞的异质性，并使用不对称方法和合成策略来研究神经保护类柠檬素。它还被用于研究转录后和翻译后修饰对真核生物翻译起始动力学的影响，并获得金（III）配合物的实体选择性。该奖项反映了美国国家科学基金会的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。